-
1
-
-
0031574019
-
Approaches to DNA mutagenesis: An overview
-
Ling, M. M. and Robinson, B. H. (1997) Approaches to DNA mutagenesis: an overview. Anal. Biochem. 254, 157–78.
-
(1997)
Anal. Biochem.
, vol.254
, pp. 157-178
-
-
Ling, M.M.1
Robinson, B.H.2
-
3
-
-
0035096836
-
General method for site-directed mutagenesis
-
Andag, R. and Schutz, E. (2001) General method for site-directed mutagenesis. BioTechniques 30, 486–8.
-
(2001)
Biotechniques
, vol.30
, pp. 486-488
-
-
Andag, R.1
Schutz, E.2
-
4
-
-
0031013799
-
Simultaneous introduction of multiple mutations using overlap extension PCR
-
Ge, L. and Rudolph, P. (1997) Simultaneous introduction of multiple mutations using overlap extension PCR. BioTechniques 22, 28–30.
-
(1997)
Biotechniques
, vol.22
, pp. 28-30
-
-
Ge, L.1
Rudolph, P.2
-
5
-
-
27544507435
-
A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR
-
An, Y., Ji, J., Wu, W., Lv, A., Huang, R., and Wei, Y. (2005) A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR. Appl. Microbiol. Biotechnol. 68, 774–8.
-
(2005)
Appl. Microbiol. Biotechnol.
, vol.68
, pp. 774-778
-
-
An, Y.1
Ji, J.2
Wu, W.3
Lv, A.4
Huang, R.5
Wei, Y.6
-
6
-
-
17844402756
-
Multiple mutagenesis of the Candida rugosa LIP1 gene and optimum production of recombinant LIP1 expressed in Pichia pastoris
-
Chang, S. W., Shieh, C. J., Lee, G. C., and Shaw, J. F. (2005) Multiple mutagenesis of the Candida rugosa LIP1 gene and optimum production of recombinant LIP1 expressed in Pichia pastoris. Appl. Microbiol. Biotechnol. 67, 215–24.
-
(2005)
Appl. Microbiol. Biotechnol.
, vol.67
, pp. 215-224
-
-
Chang, S.W.1
Shieh, C.J.2
Lee, G.C.3
Shaw, J.F.4
-
7
-
-
0031579372
-
Splicing by overlap extension by PCR using asymmetric amplification: An improved technique for the generation of hybrid proteins of immunological interest
-
Warrens, A. N., Jones, M. D., and Lechetler, R. I. (1997) Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest. Gene 186, 29–35.
-
(1997)
Gene
, vol.186
, pp. 29-35
-
-
Warrens, A.N.1
Jones, M.D.2
Lechetler, R.I.3
-
8
-
-
0033039205
-
Bridge-overlap-extension PCR method for constructing chimeric genes
-
Mehta, R. K. and Singh, J. (1999) Bridge-overlap-extension PCR method for constructing chimeric genes. BioTechniques 26, 1082–6.
-
(1999)
Biotechniques
, vol.26
, pp. 1082-1086
-
-
Mehta, R.K.1
Singh, J.2
-
9
-
-
0037082166
-
PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors
-
Kuwayama, H., Obara, S., Morio, T., Katoh, M., Urushihara, H., and Tanaka, Y. (2002) PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acids Res. 30, e2.
-
(2002)
Nucleic Acids Res
, vol.30
, pp. e2
-
-
Kuwayama, H.1
Obara, S.2
Morio, T.3
Katoh, M.4
Urushihara, H.5
Tanaka, Y.6
-
10
-
-
17144450611
-
Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously
-
Shevchuk, N. A., Bryksin, A. V., Nusinovich, Y. A., Cabello, F. C., Sutherland M., and Ladisch, S. (2004) Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously. Nucleic Acids Res. 32, e19.
-
(2004)
Nucleic Acids Res
, vol.32
, pp. e19
-
-
Shevchuk, N.A.1
Bryksin, A.V.2
Nusinovich, Y.A.3
Cabello, F.C.4
Sutherland, M.5
Ladisch, S.6
-
11
-
-
0024520745
-
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
-
Ho S. N., Hunt, H. D., Horton, R. M., Pullen J. K., and Pease, L. R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51–9.
-
(1989)
Gene
, vol.77
, pp. 51-59
-
-
Ho, S.N.1
Hunt, H.D.2
Horton, R.M.3
Pullen, J.K.4
Pease, L.R.5
-
12
-
-
34247354935
-
Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis
-
Xiao, Y. H., Yin, M. H., Hou, L., Luo, M., and Pei, Y. (2007) Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis. Biotechnol. Lett. 29, 925–30.
-
(2007)
Biotechnol. Lett.
, vol.29
, pp. 925-930
-
-
Xiao, Y.H.1
Yin, M.H.2
Hou, L.3
Luo, M.4
Pei, Y.5
|