Bridge-overlap-extension PCR method for constructing chimeric genes

BioTechniques

Volumn 26, Issue 6, 1999, Pages 1082-1086

  Mehta, Raj Kumar ^a   Singh, Jagmohan ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; GENE PRODUCT; OLIGONUCLEOTIDE;

ARTICLE; CHIMERA; EXPRESSION VECTOR; GENE CONSTRUCT; MOLECULAR GENETICS; NONHUMAN; POLYMERASE CHAIN REACTION; RNA SPLICING; SCHIZOSACCHAROMYCES POMBE; SEQUENCE ANALYSIS; STREPTOCOCCUS; TECHNIQUE;

EID: 0033039205     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99266bm17     Document Type: Article

References (5)

1. Horton, R.M., H.D. Hunt, J.K. Pullen and L.R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
2. Imai, Y. and M. Yamamoto. 1994. The fission yeast mating pheromone P-factor: its molecular structure, gene structure, and ability to induce gene expression and G 1 arrest in the mating partner. Genes Dev. 8:328-338.
3. Malke, H., B. Roe and J.J. Ferretti. 1985. Nucleotide sequence of the streptokinase gene from Streptococcus equisimilis H46A. Gene 34:357-362.
4. Mullis, K.B. and F. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350.
5. Sarkar, G. and S.S. Sommer. 1990. The "megaprimer" method of site-directed mutagenesis. BioTechniques 8:404-407.