SOX2 is required for adult human müller stem cell survival and maintenance of progenicity in vitro

Investigative Ophthalmology and Visual Science

Volumn 52, Issue 1, 2011, Pages 136-145

  Bhatia, Bhairavi ^a   Singhal, Shweta ^a   Tadman, Daniel N ^a   Khaw, Peng T ^a   Limb, G Astrid ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN; BETA3 TUBULIN; CASPASE; CELL MARKER; GREEN FLUORESCENT PROTEIN; KI 67 ANTIGEN; PROTEIN HUD; PROTEIN ISL1; RHODOPSIN; TRANSCRIPTION FACTOR PAX6; TRANSCRIPTION FACTOR POU4F2; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG; VIMENTIN; BIOLOGICAL MARKER; MESSENGER RNA; SMALL INTERFERING RNA; SOX2 PROTEIN, HUMAN; TRANSCRIPTION FACTOR SOX;

ADULT; APOPTOSIS; ARTICLE; CELL STRUCTURE; CELL SURVIVAL; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; GLIA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MUELLER STEM CELL; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NICK END LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; RETINA NERVE CELL; STEM CELL; UPREGULATION; ADULT STEM CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CYTOLOGY; ELECTROPORATION; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; GLIA; METABOLISM; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ADULT STEM CELLS; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CASPASES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; DOWN-REGULATION; ELECTROPORATION; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE SILENCING; HUMANS; IN SITU NICK-END LABELING; KI-67 ANTIGEN; NEUROGLIA; RETINAL NEURONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; SOXB1 TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 79952235212     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.10-5208     Document Type: Article

References (38)

1. Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature. 1987;328:131-136.
2. Raymond PA, Barthel LK, Bernardos RL, Perkowski JJ. Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Dev Biol. 2006;6:36.
3. Fischer AJ, Reh TA. Muller glia are a potential source of neural regeneration in the postnatal chicken retina. Nat Neurosci. 2001; 4:247-252.
4. Das AV, Mallya KB, Zhao X, et al. Neural stem cell properties of Muller glia in the mammalian retina: regulation by Notch and Wnt signaling. Dev Biol. 2006;299:283-302.
5. Ooto S, Akagi T, Kageyama R, et al. Potential for neural regeneration after neurotoxic injury in the adult mammalian retina. Proc Natl Acad Sci USA. 2004;101:13654-13659.
6. Osakada F, Ooto S, Akagi T, Mandai M, Akaike A, Takahashi M. Wnt signaling promotes regeneration in the retina of adult mammals. J Neurosci. 2007;27:4210-4219.
7. Karl MO, Hayes S, Nelson BR, Tan K, Buckingham B, Reh TA. Stimulation of neural regeneration in the mouse retina. Proc Natl Acad Sci USA. 2008;105:19508-19513.
8. Lawrence JM, Singhal S, Bhatia B, et al. MIO-M1 cells and similar Muller glial cell lines derived from adult human retina exhibit neural stem cell characteristics. Stem Cells. 2007;25:2033-2043.
9. Bhatia B, Singhal S, Lawrence JM, Khaw PT, Limb GA. Distribution of Muller stem cells within the neural retina: evidence for the existence of a ciliary margin-like zone in the adult human eye. Exp Eye Res. 2009;89:373-382.
10. Pevny LH, Lovell-Badge R. Sox genes find their feet. Curr Opin Genet Dev. 1997;7:338-344.
11. Kishi M, Mizuseki K, Sasai N, et al. Requirement of Sox2-mediated signaling for differentiation of early Xenopus neuroectoderm. Development. 2000;127:791-800.
12. Okubo T, Pevny LH, Hogan BL. Sox2 is required for development of taste bud sensory cells. Genes Dev. 2006;20:2654-2659.
13. Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R. Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 2003;17:126-140.
14. Gontan C, de Munck A, Vermeij M, Grosveld F, Tibboel D, Rottier R. Sox2 is important for two crucial processes in lung development: branching morphogenesis and epithelial cell differentiation. Dev Biol. 2008;317:296-309.
15. Komitova M, Eriksson PS. Sox-2 is expressed by neural progenitors and astroglia in the adult rat brain. Neurosci Lett. 2004;369:24-27.
16. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861-872.
17. Van Raay TJ, Moore KB, Iordanova I, et al. Frizzled 5 signaling governs the neural potential of progenitors in the developing Xenopus retina. Neuron. 2005;46:23-36.
18. Taranova OV, Magness ST, Fagan BM, et al. SOX2 is a dose-dependent regulator of retinal neural progenitor competence. Genes Dev. 2006;20:1187-1202.
19. Lin YP, Ouchi Y, Satoh S, Watanabe S. Sox2 plays a role in the induction of amacrine and Muller glial cells in mouse retinal progenitor cells. Invest Ophthalmol Vis Sci. 2009;50:68-74.
20. Ferri AL, Cavallaro M, Braida D, et al. Sox2 deficiency causes neurodegeneration and impaired neurogenesis in the adult mouse brain. Development. 2004;131:3805-3819.
21. Singhal S, Lawrence JM, Bhatia B, et al. Chondroitin sulfate pro-teoglycans and microglia prevent migration and integration of grafted Muller stem cells into degenerating retina. Stem Cells. 2008;26:1074-1082.
22. Limb GA, Matter K, Murphy G, et al. Matrix metalloproteinase-1 associates with intracellular organelles and confers resistance to lamin A/C degradation during apoptosis. Am J Pathol. 2005;166: 1555-1563.
23. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res. 2000;259:308-313.
24. Hanson SM, Gurevich EV, Vishnivetskiy SA, Ahmed MR, Song X, Gurevich VV. Each rhodopsin molecule binds its own arrestin. Proc Natl Acad Sci USA. 2007;104:3125-3128.
25. Xiang M. Requirement for Brn-3b in early differentiation of postmitotic retinal ganglion cell precursors. Dev Biol. 1998;197:155-169.
26. Pan L, Deng M, Xie X, Gan L. ISL1 and BRN3B co-regulate the differentiation of murine retinal ganglion cells. Development. 2008;135:1981-1990.
27. Lamba DA, Karl MO, Ware CB, Reh TA. Efficient generation of retinal progenitor cells from human embryonic stem cells. Proc Natl Acad Sci USA. 2006;103:12769-12774.
28. Villa P, Kaufmann SH, Earnshaw WC. Caspases and caspase inhibitors. Trends Biochem Sci. 1997;22:388-393.
29. Inoue M, Kamachi Y, Matsunami H, Imada K, Uchikawa M, Kon-doh H. PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system development. Genes Cells. 2007;12:1049-1061.
30. Agathocleous M, Iordanova I, Willardsen MI, et al. A directional Wnt/beta-catenin-Sox2-proneural pathway regulates the transition from proliferation to differentiation in the Xenopus retina. Development. 2009;136:3289-3299.
31. Wilson M, Koopman P. Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators. Curr Opin Genet Dev. 2002;12:441-446.
32. Inoue M, Iida A, Satoh S, Kodama T, Watanabe S. COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells. Exp Eye Res. 2010;90:49-56.
33. Inoue T, Hojo M, Bessho Y, Tano Y, Lee JE, Kageyama R. Math3 and NeuroD regulate amacrine cell fate specification in the retina. Development. 2002;129:831-842.
34. Cherry TJ, Trimarchi JM, Stadler MB, Cepko CL. Development and diversification of retinal amacrine interneurons at single cell resolution. Proc Natl Acad Sci USA. 2009;106:9495-9500.
35. Ferrara L, Engstrom JU, Schwartz T, Parekh-Olmedo H, Kmiec EB. Recovery of cell cycle delay following targeted gene repair by oligonucleotides. DNA Repair (Amst). 2007;6:1529-1535.
36. Lepik D, Jaks V, Kadaja L, Varv S, Maimets T. Electroporation and carrier DNA cause p53 activation, cell cycle arrest, and apoptosis. Anal Biochem. 2003;318:52-59.
37. Cavallaro M, Mariani J, Lancini C, et al. Impaired generation of mature neurons by neural stem cells from hypomorphic Sox2 mutants. Development. 2008;135:541-557.
38. Graham V, Khudyakov J, Ellis P, Pevny L. SOX2 functions to maintain neural progenitor identity. Neuron. 2003;39:749-765.