Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media

Biochemical and Biophysical Research Communications

Volumn 406, Issue 1, 2011, Pages 20-24

  Long, Lee Hua ^a   Halliwell, Barry ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Ketoglutarate; Ascorbate; Cell culture; Dulbecco's Modified Eagle's Medium; Epigallocatechin gallate; Hydrogen peroxide

Indexed keywords

2 OXOGLUTARIC ACID; ASCORBIC ACID; EPIGALLOCATECHIN GALLATE; HYDROGEN PEROXIDE; SCAVENGER; SUCCINIC ACID;

ARTICLE; ARTIFACT; CELL CULTURE; CELL GROWTH; CELL TYPE; CULTURE MEDIUM; MEDICAL LITERATURE; PRIORITY JOURNAL;

ANIMALS; ARTIFACTS; ASCORBIC ACID; CATECHIN; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA; HYDROGEN PEROXIDE; KETOGLUTARIC ACIDS; OXIDATION-REDUCTION; SUCCINIC ACID;

EID: 79952190949     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.01.091     Document Type: Article

References (35)

1. Halliwell B. Oxidative stress in cell culture: an under-appreciated problem? FEBS Lett. 2003, 540:3-6.
2. Long L.H., Clement M.V., Halliwell B. Artifacts in cell culture: rapid generation of hydrogen peroxide on addition of (-)-epigallocatechin, (-)-epigallocatechin gallate, (+)-catechin, and quercetin to commonly used cell culture media. Biochem. Biophys. Res. Commun. 2000, 273:50-53.
3. Lapidot T., Walker M.D., Kanner J. Can apple antioxidants inhibit tumor cell proliferation? Generation of H2O2 during interaction of phenolic compounds with cell culture media. J. Agric. Food. Chem. 2002, 50:3156-3160.
4. Chai P.C., Long L.H., Halliwell B. Contribution of hydrogen peroxide to the cytotoxicity of green tea and red wines. Biochem. Biophys. Res. Commun. 2002, 304:650-654.
5. Wee L.M., Long L.H., Whiteman M., Halliwell B. Factors affecting the ascorbate- and phenolic-dependent generation of hydrogen peroxide in Dulbecco's Modified Eagle's Medium. Free Radic. Res. 2003, 37:1123-1130.
6. Clement M.V., Ramalingam J., Long L.H., Halliwell B. The in vitro cytotoxicity of ascorbate depends on the culture medium used to perform the assay and involves hydrogen peroxide. Antioxid. Redox Signal. 2001, 3:157-163.
7. Elbing L., Weiss R.M., Teufelhofer O., Uhl M., Knasmueller S., Schulte-Hermann R., Berger W., Micksche M. Green tea extract and (-)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities. FASEB J. 2005, 19:807-809.
8. Lee K.W., Hur H.J., Lee H.H., Lee C.Y. Antiproliferative effects of dietary phenolic substances and hydrogen peroxide. J. Agric. Food. Chem. 2005, 53:1990-1995.
9. Halliwell B. Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies?. Arch. Biochem. Biophys. 2008, 476:107-112.
10. Long L.H., Kirkland D., Whitwell J., Halliwell B. Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium. Mutat. Res. 2007, 634:177-183.
11. Long L.H., Hoi A., Halliwell B. Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media. Arch. Biochem. Biophys. 2010, 501:162-169.
12. Miwa H., Fujii J., Kanno H., Taniguchi N., Aozasa K. Pyruvate secreted by human lymphoid cell lines protects cells from hydrogen peroxide mediated cell death. Free Radic. Res. 2000, 33:45-56.
13. Herz H., Blake D.R., Grootveld M. Multicomponent investigations of the hydrogen peroxide- and hydroxyl radical-scavenging antioxidant capacities of biofluids: the roles of endogenous pyruvate and lactate. Relevance to inflammatory joint diseases. Free Radic. Res. 1997, 26:19-35.
14. Babich H., Liebling E.J., Burger R.F., Zuckerbraun H.L., Schuck A.G. Choice of DMEM, formulated with or without pyruvate, plays an important role in assessing the in vitro cytotoxicity of oxidants and prooxidant nutraceuticals. In Vitro Cell Dev. Biol. 2009, 45:226-233.
15. Nath K.A., Enright H., Nutter L., Fischereder M., Zou J.N., Hebbel R.P. Effect of pyruvate on oxidant injury to isolated and cellular DNA. Kidney Int. 1994, 45:166-176.
16. Varma S.D., Morris S.M. Peroxide damage to the eye lens in vitro prevention by pyruvate. Free Radic. Res. 1998, 4:283-290.
17. Giandomenico A.R., Cerniglia G.E., Biaglow J.E., Stevens C.W., Koch C.J. The importance of sodium pyruvate in assessing damage produced by hydrogen peroxide. Free Radic. Biol. Med. 1997, 23:426-434.
18. Salahudeen A.K., Clark E.C., Nath K.A. Hydrogen peroxide-induced renal injury. J. Clin. Invest. 1991, 88:1886-1893.
19. Andrae U., Sing J., Ziegler-Skylakakis K. Pyruvate and related α-ketoacids protect mammalian cells in culture against hydrogen peroxide-induced cytotoxicity. Toxicol. Lett. 1985, 28:93-98.
20. Hebbel R.P., Croatt A.J., Zhou B.J., Nutter L.M. α-Ketoacids scavenge H2O2 in vitro and in vivo and reduce menadione-induced DNA injury and cytotoxicity. Am. J. Physiol. 1995, 268:C227-C236.
21. Long L.H., Halliwell B. Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media. Biochem. Biophys. Res. Commun. 2009, 388:700-704.
22. O'Donnell-Tormey J., Nathan C.F., Lanks K., DeBoer C.J., de la Harpe J. Secretion of pyruvate. An antioxidant defense of mammalian cells. J. Exp. Med. 1987, 165:500-514.
23. Perera A., Parkes H.G., Herz H., Haycock P., Blake D.R., Grootveld M.C. High resolution 1H NMR investigations of the reactivities of alpha-keto acid anions with hydrogen peroxide. Free Radic. Res. 1997, 26:145-157.
24. Ramachandran S., Morris S.M., Devamanoharan P., Henein M., Varma S.D. Radio-isotopic determination of hydrogen peroxide in aqueous humor and urine. Exp. Eye Res. 1991, 53:503-506.
25. Varma S.D., Devamanoharan P.S. Excretion of hydrogen peroxide in human urine. Free Radic. Res. Commun. 1990, 8:73-78.
26. Fuchs M., Gerlach J., Unger J., Enck J., Smith M., Neuhaus P., Nündel M., Riedel E. Alpha-keto acid metabolism by hepatocytes cultured in a hybrid liver support bioreactor. Int. J. Artif. Organs 1994, 17:554-558.
27. Long L.H., Evans P.J., Halliwell B. Hydrogen peroxide in human urine: implications for antioxidant defense and redox regulation. Biochem. Biophys. Res. Commun. 1999, 262:605-609.
28. B. Halliwell, Free radicals and antioxidants - quo vadis? Trends Pharmacol. Sci., in press, doi:10.1016/j.tips.2010.12.002.
29. Clement M.V., Long L.H., Ramalingam J., Halliwell B. The cytotoxicity of dopamine may be an artefact of cell culture. J. Neurochem. 2002, 81:414-421.
30. Ayres K.N. High cloning efficiency of human lymphoid cells in agarose without feeder layers. J. Natl Cancer Inst. 1982, 68:919-923.
31. Nilsang S., Kumar A., Rakshit S.K. Effect of alpha-ketoglutarate on monoclonal antibody production of hybridoma cell lines in serum-free and serum-containing medium. Appl. Biochem. Biotechnol. 2008, 151:489-501.
32. Facci L., Skaper S.D., Varon S. Specific replacements of pyruvate for trophic support of central and peripheral nervous system neurons. J. Neurochem. 1958, 45:926-934.
33. Juurlink B.H., Munoz D.G., Ang L.C. Motoneuron survival in vitro: effects of pyruvate, alpha-ketoglutarate, gangliosides and potassium. Neurosci. Lett. 1991, 133:25-28.
34. Nilsang S., Nehru V., Plieva F.M., Nandakumar K.S., Rakshit S.K., Holmdahl R., Mattiasson B., Kumar A. Three-dimensional culture for monoclonal antibody production by hybridoma cells immobilized in macroporous gel particles. Biotechnol. Prog. 2008, 24:1122-1131.
35. Pine L., Hoffman P.S., Malcolm G.B., Benson R.F., Franzus M.J. Role of keto acids and reduced-oxygen-scavenging enzymes in the growth of Legionella species. J. Clin. Microbiol. 1986, 23:33-42.