Factors affecting the ascorbate- and phenolic-dependent generation of hydrogen peroxide in Dulbecco's Modified Eagles Medium

Free Radical Research

Volumn 37, Issue 10, 2003, Pages 1123-1130

  Wee, Liang Meng ^a   Long, Lee Hua ^a   Whiteman, Matthew ^a   Halliwell, Barry ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Ascorbate; Cell culture; Dulbecco's Modified Eagles Medium; Flavonoid; Gallic acid; Hydrogen peroxide

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; ASCORBIC ACID; BUFFER; CARBON DIOXIDE; CHELATING AGENT; DEFEROXAMINE; EGTAZIC ACID; EPICATECHIN; EPIGALLOCATECHIN GALLATE; GALLIC ACID; HYDROGEN PEROXIDE; METAL ION; PENTETIC ACID; PHENANTHROLINE DERIVATIVE; PHENOL DERIVATIVE; POLYPHENOL DERIVATIVE; QUERCETIN;

ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; INCUBATION TIME; OXIDATION; PH; TEMPERATURE;

ASCORBIC ACID; CATALASE; CELL LINE, TUMOR; CELLS, CULTURED; CHELATING AGENTS; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; EDETIC ACID; EGTAZIC ACID; GALLIC ACID; HUMANS; HYDROGEN PEROXIDE; HYDROGEN-ION CONCENTRATION; IONS; PENTETIC ACID; SPECTROPHOTOMETRY, ATOMIC; TEMPERATURE; TIME FACTORS;

CICONIIFORMES;

EID: 0142125716     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715760310001607041     Document Type: Article

References (25)

1. Long, L.H., Clement, M.V. and Halliwell, B. (2000) "Artifacts in cell culture: rapid generation of hydrogen peroxide on addition of (-)-epigallocatechin, (-)-epigallocatechin gallate, (+)-catechin and quercetin to commonly used cell culture media", Biochem. Biophys. Res. Commun. 273, 50-53.
2. Long, L.H. and Halliwell, B. (2001) "Oxidation and generation of hydrogen peroxide by thiol compounds in commonly used cell culture media", Biochem. Biophys. Res. Commun. 286,991-994.
3. Clement, M.V, Ramalingam, J., Long, L.H. and Halliwell, B. (2001) "The in vitro cytotoxicity of ascorbate depends on the culture medium used to perform the assay and involves hydrogen peroxide", Antiox. Redox Signal. 3, 157-163.
4. Clement, M.V, Long, L.H., Ramalingam, J. and Halliwell, B. (2002) "The cytotoxicity of dopamine may be an artefact of cell culture", J. Neurochem. 81, 414-421.
5. 2 during interaction of phenolic compounds with cell culture media", J. Agric. Food Chem. 50, 3156-3160.
6. 2 generation in CaCo - 2 cells culture medium by addition of phenolic compounds: effect of ascorbic acid", Free Radic. Res. 36, 593-599.
7. 2 and methionine sulfoxide by epigallocatechin gallate and antioxidants", Anticancer Res. 21, 2633-2642.
8. 2 formation in cytotoxicity of flavonols", Biosci. Biotechnol. Biochem. 65,1227-1229.
9. Bartosz, G. (2000) "ROS are formed in cell culture media", Acta Biochim. Pol. 47,1197-1198.
10. 2 at physiologically relevant EGCG contributions", Biochem. Biophys. Res. Commun. 296,584-588.
11. Isuzugawa, K., Inoue, M. and Ogihara, Y. (2001) "Catalase contents in cells determine sensitivity to the apoptosis inducer gallic acid", Biol. Pharm. Bull. 24,1022-1026.
12. Chai, P.C., Long, L.H. and Halliwell, B. (2003) "Contribution of hydrogen peroxide to the cytotoxicity of green tea and red wines", Biochem. Biophys. Res. Commun. 304, 650-654.
13. Peterszegi, G., Dagonet, EB., Labat-Robert, J. and Robert, L. (2002) "Inhibition of cell proliferation and fibronectin biosynthesis by Na ascorbate", Eur. J. Clin. Investig. 32, 372-380.
14. 2 production in transformed and non-transformed human bronchial cell lines. Possible mechanisms of cell growth inhibition and apoptosis induction", Carcinogenesis 21, 2035-2039.
15. Whiteman, M., Hooper, D.C., Scott, G.S., Koprowski, H. and Halliwell, B. (2002) "Inhibition of hypochlorous acid-mediated cytotoxicity by nitrite", Proc. Natl Acad. Sci. USA 99,12061-12066.
16. 2O'2, 4-HNE or serum deprivation: potentiation by Bcl-2", J. Neurochem. 78, 209-220.
17. Long, L.H., Evans, P.J. and Halliwell, B. (1999) "Hydrogen peroxide in human urine: implications for antioxidant defense and redox regulation", Biochem. Biophys. Res. Commun. 262, 605-609.
18. Halliwell, B. and Gutteridge, J.M.C. (1999) Free Radicals in Biology and Medicine, 2nd ed. (Oxford University Press, Oxford, UK).
19. Evans, P.J., Tredger, J.M., Dunne, J.B. and Halliwell, B. (1996) "Catalytic metal ions and the loss of reduced glutathione from University of Wisconsin preservation solution", Transplantation 62, 1046-1049.
20. Evans, P.J. and Halliwell, B. (1994) "Measurement of iron and copper in biological systems: bleomycin and copper-phenanthroline assays", Methods Enzymol. 233, 82-92.
21. Laughton, M.J., Halliwell, B., Evans, P.J. and Hoult, J.R. (1989) "Antioxidant and pro-oxidant actions of the plant phenolics quercetin, gossypol and myricetin. Effects on lipid peroxidation, hydroxyl radical generation and bleomycin-dependent damage to DNA", Biochem. Pharmacol. 17, 2859-2865.
22. Halliwell, B. (1989) "Protection against tissue damage in vivo by desferrioxamine: what is its mechanism of action?", Free Radic. Biol. Med. 7,645-651.
23. Dizdaroglu, M., Aruoma, O.I. and Halliwell, B. (1990) "Modification of bases in DNA by copper ion-1, 10-phenanthroline complexes", Biochemistry 29, 8447-8451.
24. 2+ ions: implications for their antioxidant properties", Biochem. J. 330,1173-1178.
25. Halliwell, B. (2003) "Oxidative stress in cell culture: an under-appreciated problem", FEBS Lett. 540, 3-6.