Coupling of dephosphorylation and nuclear export of smads in TGF-β signaling

Methods in Molecular Biology

Volumn 647, Issue , 2010, Pages 125-137

  Dai, Fangyan ^a   Duan, Xueyan ^a   Liang, Yao Yun ^a   Lin, Xia ^a   Feng, Xin Hua ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Nuclear export; Nuclear phosphatase; PPM1A; RanBP3; Signal transduction; Smad

Indexed keywords

EUKARYOTA;

PHOSPHATASE; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

ACTIVE TRANSPORT; ARTICLE; CELL FRACTIONATION; CELL LINE; CELL NUCLEUS; HUMAN; INTRACELLULAR SPACE; METABOLISM; PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ACTIVE TRANSPORT, CELL NUCLEUS; CELL FRACTIONATION; CELL LINE; CELL NUCLEUS; HUMANS; INTRACELLULAR SPACE; PHOSPHORIC MONOESTER HYDROLASES; PHOSPHORYLATION; SIGNAL TRANSDUCTION; SMAD PROTEINS; TRANSFORMING GROWTH FACTOR BETA;

EID: 79952114462     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-738-9_7     Document Type: Article

References (20)

1. Feng XH, Derynck R (2005) Specificity and versatility in tgf-beta signaling through Smads. Annu Rev Cell Dev Biol 21:659-693 (Pubitemid 41740650)
2. Massague J, Seoane J, Wotton D (2005) Smad transcription factors. Genes Dev 19:2783-2810 (Pubitemid 41739965)
3. Lin X, Duan X, Liang YY et al (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell 125:915-928 (Pubitemid 43795197)
4. Watanabe M, Masuyama N, Fukuda M, Nishida E (2000) Regulation of intracellular dynamics of Smad4 by its leucine-rich nuclear export signal. EMBO Rep 1:176-182
5. Pierreux CE, Nicolas FJ, Hill CS (2000) Transforming growth factor beta-independent shuttling of Smad4 between the cytoplasm and nucleus. Mol Cell Biol 20:9041-9054 (Pubitemid 32245933)
6. Inman GJ, Nicolas FJ, Hill CS (2002) Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Mol Cell 10:283-294 (Pubitemid 35007343)
7. Nicolas FJ, De Bosscher K, Schmierer B, Hill CS (2004) Analysis of Smad nucleocytoplasmic shuttling in living cells. J Cell Sci 117:4113-4125 (Pubitemid 39328299)
8. Schmierer B, Hill CS (2005) Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads. Mol Cell Biol 25:9845-9858 (Pubitemid 41572734)
9. Xiao Z, Brownawell AM, Macara IG, Lodish HF (2003) A novel nuclear export signal in Smad1 is essential for its signaling activity. J Biol Chem 278:34245-34252 (Pubitemid 37553267)
10. Kurisaki A, Kose S, Yoneda Y, Heldin CH, Moustakas A (2001) Transforming growth factor-beta induces nuclear import of Smad3 in an importin-beta1 and Ran-dependent manner. Mol Biol Cell 12:1079-1091 (Pubitemid 33052000)
11. Xu L, Chen YG, Massague J (2000) The nuclear import function of Smad2 is masked by SARA and unmasked by TGFbeta-dependent phosphorylation. Nat Cell Biol 2:559-562
12. Xu L, Kang Y, Col S, Massague J (2002) Smad2 nucleocytoplasmic shuttling by nucleoporins CAN/Nup214 and Nup153 feeds TGFbeta signaling complexes in the cytoplasm and nucleus. Mol Cell 10:271-282 (Pubitemid 35007342)
13. Coburn GA, Wiegand HL, Kang Y, Ho DN, Georgiadis MM, Cullen BR (2001) Using viral species specificity to define a critical protein/RNA interaction surface. Genes Dev 15:1194-1205 (Pubitemid 32472749)
14. Cullen BR (2004) Assaying nuclear messenger RNA export in human cells. Methods Mol Biol 257:85-92
15. Dai F, Lin X, Chang C, Feng X-H (2009) Nuclear export of Smad2 and Smad3 by RanBP3. Dev Cell 16:345-357
16. Englmeier L, Fornerod M, Bischoff FR, Petosa C, Mattaj IW, Kutay U (2001) RanBP3 influences interactions between CRM1 and its nuclear protein export substrates. EMBO Rep 2:926-932 (Pubitemid 33040582)
17. Wu JW, Hu M, Chai J et al (2001) Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling. Mol Cell 8:1277-1289 (Pubitemid 34084999)
18. Duan X, Liang YY, Feng XH, Lin X (2006) Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway. J Biol Chem 281:36526-36532 (Pubitemid 46042119)
19. Feng XH, Derynck R (1996) Ligandindependent activation of transforming growth factor (TGF) beta signaling pathways by heteromeric cytoplasmic domains of TGFbeta receptors. J Biol Chem 271:13123-13129 (Pubitemid 26175889)
20. Wieser R, Wrana JL, Massague J (1995) GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex. EMBO J 14:2199-2208