Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity

Methods in Molecular Biology

Volumn 648, Issue , 2010, Pages 291-301

  Cerioni, Liana ^a   Cantoni, Orazio ^a  

^a NONE

Author keywords

Bad; Inflammationsub cellular localization; Mitochondria; Mitochondrial permeability transition; Peroxynitrite; Western blot analysis

Indexed keywords

ACTIN; CHAPERONIN 60; PEROXYNITRITE; PROTEIN BAD; CARRIER PROTEIN; CHAPERONIN; MITOCHONDRIAL PERMEABILITY TRANSITION PORE; PEROXYNITROUS ACID;

CELL CULTURE; CELL FRACTIONATION; CELL SURVIVAL; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSOL; HUMAN; HUMAN CELL; INFLAMMATION; MACROPHAGE; MITOCHONDRIAL PERMEABILITY; MITOCHONDRION; MONOCYTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SYSTEMATIC REVIEW; WESTERN BLOTTING; ARTICLE; BIOCHEMISTRY; CELL LINE; DRUG EFFECT; METABOLISM; METHODOLOGY; PROTEIN TRANSPORT;

ACTINS; BCL-ASSOCIATED DEATH PROTEIN; BIOCHEMISTRY; BLOTTING, WESTERN; CELL LINE; CHAPERONIN 60; CYTOSOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; PEROXYNITROUS ACID; PROTEIN TRANSPORT; SUBCELLULAR FRACTIONS;

EID: 79952033274     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-756-3_20     Document Type: Chapter

References (13)

1. Adams J, Cory S (1998) The Bcl-2 protein family: arbiters of cell survival. Science 281:1322–1326
2. Zamzami BN, Susin SA, Marchetti P, Hirsch T, Gómez-Monterrey I, Castedo M, Kroemer G (1996) Mitochondrial control of nuclear apoptosis. J Exp Med 183:1533–1544
3. Datta SR, Dudek H, Tao X, Masters S, Fu H, Gotoh Y, Greenberg ME (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91:231–241
4. del Peso L, Gonzalez-Garcia M, Page C, Herrera R, Nunez G (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278:687–689
5. Tan Y, Ruan H, Demeter MR, Comb MJ (1999) p90(RSK) blocks Bad-mediated cell death via a protein kinase C-dependent pathway. J Biol Chem 274:34859–34867
6. Bertolotto C, Maulon L, Filippa N, Baier G, Auberger P (2000) Protein kinase C theta and epsilon promote T-cell survival by a rsk-dependent phosphorylation and inactivation of BAD. J Biol Chem 275:37246–37250
7. Datta SR, Katsov A, Hu L, Petros A, Fesik SW, Yaffem MB, Greenberg ME (2000) 14-3-3 proteins and survival kinases cooperate to inactivate BAD by BH3 domain phosphorylation. Mol Cell 6:41–51
8. Guidarelli A, Cerioni L, Tommasini I, Fiorani M, Brüne B, Cantoni O (2005) Role of Bcl-2 in the arachidonate-mediated survival signaling preventing mitochondrial permeability transition-dependent U937 cell necrosis induced by peroxynitrite. Free Rad Biol Med 39:1638–1649
9. Cerioni L, Palomba L, Brüne B, Cantoni O (2006) Peroxynitrite-induced mitochondrial translocation of PKCα causes U937 cell survival. Biochem Biophys Res Commun 339:126–131
10. Tommasini I, Palomba L, Guidarelli A, Cerioni L, Cantoni O (2006) 5-Hydroxyeicosatetraenoic acid is a key intermediate of the arachidonate-dependent protective signaling in monocytes/ macrophages exposed to peroxynitrite. J Leukoc Biol 80:929–938
11. Tommasini I, Cerioni L, Palomba L, Cantoni O (2008) Prostaglandin E2 signals monocyte/macrophage survival to peroxynitrite via protein kinase A converging in Bad phosphorylation with the protein kinase Cα-dependent pathway driven by 5-hydroxyeicosatetraenoic acid. J Immunol 181:5637–5645
12. Radi R, Beckman JS, Bush KM, Freeman BA (1991) Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J Biol Chem 266:4244–4250
13. 2 in U937 cells: the extent of arachidonic acid formation regulates the balance between cell survival or death. Cell Death Differ 9:1368–1376