Stress response profiles in human fibroblasts exposeto heat shock or oxidative stress

Methods in Molecular Biology

Volumn 648, Issue , 2010, Pages 161-173

  Pedersen, Christina Bak ^a   Gregersen, Niels ^a  

^a NONE

Author keywords

Heat shock; Heat shock protein 70 (Hsp70); Heme oxygenase 1 (HO1); Oxidative stress; Quantitative PCR; Western blotting

Indexed keywords

HEAT SHOCK PROTEIN 70; HEME OXYGENASE 1; HYDROGEN PEROXIDE; MESSENGER RNA; COMPLEMENTARY DNA; HEAT SHOCK PROTEIN; RNA;

FIBROBLAST; HEAT SHOCK; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PROTEIN EXPRESSION; REGULATORY MECHANISM; UPREGULATION; WESTERN BLOTTING; ARTICLE; BIOSYNTHESIS; CELL CULTURE; GENE EXPRESSION REGULATION; GENETICS; HEAT SHOCK RESPONSE; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

BLOTTING, WESTERN; CELLS, CULTURED; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBROBLASTS; GENE EXPRESSION REGULATION; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE; HUMANS; MOLECULAR BIOLOGY; OXIDATIVE STRESS; POLYMERASE CHAIN REACTION; RNA;

EID: 79952014822     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-756-3_10     Document Type: Chapter

References (18)

1. Richter-Landsberg C, Goldbaum O (2003) Stress proteins in neural cells: functional roles in health and disease. Cell Mol Life Sci 60:337–349
2. Unno K, Asakura H, Shibuya Y, Kaihou M, Fukatsu H, Okada S, Oku N (2002) Stress response caused by chronic alcohol intake in aged rat brain. Alcohol Clin Exp Res 26:1017–1023
3. Verbeke P, Fonager J, Clark BF, Rattan SI (2001) Heat shock response and ageing: mechanisms and applications. Cell Biol Int 25:845–857
4. Westerheide SD, Morimoto RI (2005) Heat shock response modulators as therapeutic tools for diseases of protein conformation. J Biol Chem 280:33097–33100
5. Calabrese V, Lodi R, Tonon C, D’Agata V, Sapienza M, Scapagnini G, Mangiameli A, Pennisi G, Stella AM, Butterfield DA (2005) Oxidative stress, mitochondrial dysfunction and cellular stress response in Friedreich’s ataxia. J Neurol Sci 233:145–162
6. Sonna LA, Fujita J, Gaffin SL, Lilly CM (2002) Invited review: Effects of heat and cold stress on mammalian gene expression. J Appl Physiol 92:1725–1742
7. Fourie AM, Peterson PA, Yang Y (2001) Characterization and regulation of the major histocompatibility complex-encoded proteins Hsp70-Hom and Hsp70-1/2. Cell Stress Chaperones 6:282–295
8. Gabai VL, Meriin AB, Yaglom JA, Volloch VZ, Sherman MY (1998) Role of Hsp70 in regulation of stress-kinase JNK: implications in apoptosis and aging. FEBS Lett 438:1–4
9. Stege GJ, Li GC, Li L, Kampinga HH, Konings AW (1994) On the role of hsp72 in heat-induced intranuclear protein aggregation. Int J Hyperthermia 10:659–674
10. Bruening W, Roy J, Giasson B, Figlewicz DA, Mushynski WE, Durham HD (1999) Up-regulation of protein chaperones preserves viability of cells expressing toxic Cu/ Zn-superoxide dismutase mutants associated with amyotrophic lateral sclerosis. J Neurochem 72:693–699
11. Elbirt KK, Bonkovsky HL (1999) Heme oxygenase: recent advances in understanding its regulation and role. Proc Assoc Am Physicians 111:438–447
12. Choi AM, Alam J (1996) Heme oxygenase-1: function, regulation, and implication of a novel stress-inducible protein in oxidant-induced lung injury. Am J Respir Cell Mol Biol 15:9–19
13. Stocker R (1990) Induction of haem oxygenase as a defence against oxidative stress. Free Radic Res Commun 9:101–112
14. Clark JE, Foresti R, Green CJ, Motterlini R (2000) Dynamics of haem oxygenase-1 expression and bilirubin production in cellular protection against oxidative stress. Biochem J 348(Pt 3):615–619
15. Poss KD, Tonegawa S (1997) Reduced stress defense in heme oxygenase 1-deficient cells. Proc Natl Acad Sci U S A 94:10925–10930
16. Scapagnini G, D’Agata V, Calabrese V, Pascale A, Colombrita C, Alkon D, Cavallaro S (2002) Gene expression profiles of heme oxygenase isoforms in the rat brain. Brain Res 954:51–59
17. Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6:986–994
18. Winer J, Jung CK, Shackel I, Williams PM (1999) Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem 270:41–49