Evaluation of a QIAamp DNA stool purification kit for Shiga-toxigenic Escherichia coli detection in bovine fecal swabs by PCR

Revista Argentina de Microbiologia

Volumn 36, Issue 1, 2004, Pages 1-5

  Gioffre A ^a   Meichtri, L ^a   Zumarraga M ^a   Rodriguez R ^a   Cataldi, A ^a  

^a INSTITUTO NACIONAL DE TECNOLOGÍA AGROPECUARIA INTA   (Argentina)

Author keywords

Bovine; PCR; Shiga toxin; STEC; Stool

Indexed keywords

DNA; SHIGA TOXIN;

ARTICLE; BACTERIUM DETECTION; CATTLE; DIAGNOSTIC TEST; DNA DETERMINATION; ESCHERICHIA COLI; FECES ANALYSIS; GENE; GENE AMPLIFICATION; GENE ISOLATION; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; STX GENE;

ANIMALS; CATTLE; ENZYME INHIBITORS; ESCHERICHIA COLI; FECES; FOOD CONTAMINATION; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RECTUM; SENSITIVITY AND SPECIFICITY; SHIGA TOXINS; TAQ POLYMERASE;

EID: 2442478241     PISSN: 03257541     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Blanco M, Blanco JE, Blanco J, Gonzalez EA, Alonso MP, Maas H, Jansen WH (1996) Prevalence and characteristics of human and bovine verotoxigenic Escherichia coli strains isolated in Galicia (north-western Spain). Eur. J. Epidemiol. 12: 13-19.
2. Brian MJ, Frosolono M, Murray BE, Miranda A, Lopez EL, Gumez HF, Cleary TG (1992) Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome. J. Clin. Microbiol. 30: 1801-1806.
3. Gannon V, Rashed M, King R, Golsteyn Thoma E (1993) Detection and characterization of the eae gene of Shiga like Toxin-producing Escherichia coli using polymerase chain reaction. J. Clin. Microbiol. 31: 1268-1274.
4. Gioffre A, Meichtri L, Miliwebsky E, Baschkier A, Cataldi A, Rodriguez R, Rivas M (2002) Evaluation of different procedures to detect STEC by PCR in healthy cattle in Argentina. Vet. Microbiol. 87: 301-313.
5. Holland JL, Louie L, Simor AE, Louie M (2000) PCR Detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA. J. Clin. Microbiol. 38: 4108-4113.
6. Monteiro L, Bonnemaison D, Vekris A, Petry K, Bonnet J, Vidal R, et al (1997) Complex polysaccharides as PCR Inhibitors in feces: Helicobacter pylori model. J. Clin. Microbiol. 35: 995-998.
7. Paton A, Paton JC, Goldwater PN, Manning PA (1993) Direct detection of Escherichia coli shiga-like toxin genes in primary fecal cultures by polymerase chain reaction. J. Clin. Microbiol. 31: 3063-3067.
8. Paton A, Paton J (1998) Detection and characterization of Shiga toxigenic Escherichia coli by using Multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36: 598-602.
9. Pollard DR, Johnson WM, Lior H, Tyler SD, Rozee KR (1990) Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J. Clin. Microbiol. 28: 540-545.
10. Verweij JJ, Blotkamp J, Brienen EAT, Aguirre A, Polderman AM (2000) Differentiation of Entamoeba histolytica and Entamoeba dispar cysts using polymerase chain reaction on DNA isolated from faeces with spin columns. Eur. J. Clin. Microbiol. Infect. Dis. 19: 358-361.
11. Wagner PL, Neely MN, Zhang X, Acheson DW, Waldor MK, Friedman DI (2001) Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain. J. Bacteriol. 183: 2081-2085.