The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography

Protein Expression and Purification

Volumn 77, Issue 1, 2011, Pages 26-33

  Stalder, Elizabeth S ^a   Nagy, Lauren H ^a   Batalla, Pilar ^a   Arthur, Terrance M ^a   Thompson, Nancy E ^a   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Beta flap domain; Epitope tag; Immunoaffinity chromatography; Polyol responsive; RNA polymerase

Indexed keywords

BACTERIAL PROTEIN; DNA DIRECTED RNA POLYMERASE; EPITOPE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PHOTOPROTEIN; POLYMER; POLYOL; RNA POLYMERASE BETA SUBUNIT; YELLOW FLUORESCENT PROTEIN, BACTERIA;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CELL STRAIN HEK293; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; DNA-DIRECTED RNA POLYMERASES; EPITOPE MAPPING; EPITOPES; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; IMMUNOSORBENT TECHNIQUES; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; POLYMERS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 79951953557     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.12.011     Document Type: Article

References (14)

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