GroEL-GroES solubilizes abundantly expressed xylulokinase in Escherichia coli

Journal of Applied Microbiology

Volumn 98, Issue 1, 2005, Pages 210-215

  Bu, S ^a   Tsang, P W K ^a   Fu, Rong Zhao ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Coexpression; GroEL GroES; Insoluble protein; Molecular chaperone; Xylulokinase

Indexed keywords

BACTERIOLOGY; BIOETHANOL; ETHANOL; LIGNOCELLULOSE; RECOMBINANT PROTEINS; SOLUBILITY;

BACILLUS MEGATERIUM; BIO-ETHANOLS; CO-EXPRESSION; COMMERCIAL PRODUCTIONS; E. COLI BL21(DE3); GROEL/GROES; INSOLUBLE PROTEINS; MOLECULAR CHAPERONES; SOLUBILITY STUDIES; XYLULOKINASE;

ESCHERICHIA COLI;

ALCOHOL; CHAPERONIN; ESCHERICHIA COLI PROTEIN; LIGNOCELLULOSE; PENTOSE PHOSPHATE; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG; XYLAN; XYLOSE; XYLULOKINASE; XYLULOSE 5 PHOSPHATE;

ENZYME; MICROBIOLOGY; PROTEIN;

ARTICLE; BACILLUS MEGATERIUM; COST; ENZYME ASSAY; ESCHERICHIA COLI; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RELIABILITY; SOLUBILITY;

BACILLUS MEGATERIUM; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 12344257694     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2004.02446.x     Document Type: Article

References (25)

1. Aristidou, A. and Penttila, M. (2000) Metabolic engineering applications to renewable resource utilization. Current Opinion in Biotechnology 11, 187-198.
2. Biely, P., Mislovicova, D. and Toman, R. (1985) Soluble chromogenic substrates for the assay of endo-1,4-beta-xylanases and endo-1,4-beta- glucanases. Annual Review of Biochemistry 144, 142-146.
3. Braig, K. (1998) Chaperonins. Current Opinion in Structural Biology 8, 159-165.
4. Bu, S. (2004) Breeding of better β-xylulokinase. PhD thesis, The Chinese University of Hong Kong, Hong Kong.
5. Ho, N.W, Chen, Z. and Brainard, A.P. (1998) Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Applied and Environmental Microbiology 64, 1852-1859.
6. Hu, S.Y., Wu, J.L. and Huang, J.H. (2004) Production of tilapia insulin-like growth factor-2 in high cell density cultures of recombinant Escherichia coli. Journal of Biotechnology 107, 161-171.
7. Ideno, A., Furutani, M., Iwabuchi, T., Iida, T., Iba, Y., Kurosawa, Y., Sakuraba, H., Ohshima, T. et al. (2004) Expression of foreign proteins in Escherichia coli by fusing with an archaeal FK506 binding protein. Applied Microbiology and Biotechnology 64, 99-105.
8. Jeffries, T.W. (1983) Utilization of xylose by bacteria, yeasts, and fungi. Advances in Biochemical Engineering and Biotechnology 27, 1-32.
9. Lee, T.H., Kim, M.D., Park, Y.C., Bae, S.M., Ryu, Y.W. and Seo, J.H. (2003) Effects of xylulokinase activity on ethanol production from D-xylulose by recombinant Saccharomyces cerevisiae. Journal of Applied Microbiology 95, 847-852.
10. Lengeler, J. (1980) Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin. Molecular Genetics and Genomics 179, 49-54.
11. Mayer, M. and Buchner, J. (2004) Refolding of inclusion body proteins. Methods in Molecular Medicine 94, 239-254.
12. Moore, J.T., Uppal, A., Maley, F. and Maley, G.F. (1993) Overcoming inclusion body formation in a high-level expression system. Protein Expression and Purification 4, 160-163.
13. Neuberger, M.S., Hartley, B.S., Walker, J.E. (1981) Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes. Biochemical Journal 193, 513-524.
14. Nishihara, K., Kanemori, M., Kitagawa, M., Yanagi, H. and Yura, T. (1998) Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli. Applied and Environmental Microbiology 64, 1694-1699.
15. Richard, P., Toivari, M.H. and Penttila, M. (2000) The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism. FEMS Microbiology Letters 190, 39-43.
16. Rygus, T., Scheler, A., Allmansberger, R. and Hillen, W. (1991) Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Archives of Microbiology 155, 535-542.
17. Sanchez, J.C., Gimenez, R., Schneider, A., Fessner, W.D., Baldoma, L., Aguilar, J. and Badia, J. (1994) Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli. Journal of Biological Chemistry 269, 29665-29669.
18. Shamanna, D.K. and Sanderson, K.E. (1979) Uptake and catabolism of D-xylose in Salmonella typhimurium LT2. Journal of Bacteriology 139, 64-70.
19. Simpsin, F.J. (1966) D-xylulokinase. Methods in Enzymology 9, 454-458.
20. Sorensen, H.P., Sperling-Petersen, H.U. and Mortensen, K.K. (2003) A favorable solubility partner for the recombinant expression of streptavidin. Protein Expression and Purification 32, 252-259.
21. Terpe, K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology 60, 523-533.
22. Thomas, J.G. and Baneyx, F. (1996) Protein folding in the cytoplasm of Escherichia coli: requirements for the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines. Molecular Microbiology 21, 1185-1196.
23. Toivari, M.H., Aristidou, A., Ruohonen, L. and Penttila, M. (2001) Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae: importance of xylulokinase (XKS1) and oxygen availability. Metabolic Engineering 3, 236-249.
24. Wheals, A.E., Basso, L.C., Alves, D.M. and Amorim, H.V. (1999) Fuel ethanol after 25 years. Trends in Biotechnology 17, 482-487.
25. Wungsintaweekul, J., Herz, S., Hecht, S., Eisenreich, W., Feicht, R., Rohdich, F., Bacher, A. and Zenk, M.H. (2001) Phosphorylation of 1-deoxy-D-xylulose by D-xylulokinase of Escherichia coli. European Journal of Biochemistry 268, 310-316.