A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species

Protein Expression and Purification

Volumn 31, Issue 1, 2003, Pages 155-160

  Bergendahl, Veit ^a   Thompson, Nancy E ^a   Foley, Kit M ^a   Olson, Brian M ^a   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; STREPTOMYCES; STREPTOMYCES COELICOLOR;

EID: 0141539471     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00145-1     Document Type: Article

References (8)

1. R.R. Burgess, N.E. Thompson, Advances in gentle immunoaffinity chromatography, Curr. Opin. Biotechnol. 13 (2002) 304.
2. N.E. Thompson, R.R. Burgess, Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies, Methods Enzymol. 274 (1996) 513-526.
3. N.E. Thompson, D.A. Hager, R.R. Burgess, Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase, Biochemistry 31 (1992) 7003-7008.
4. N.E. Thompson, D.B. Aronson, R.R. Burgess, Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody, J. Biol. Chem. 265 (1990) 7069-7077.
5. N.E. Thompson, R.R. Burgess, Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF, Protein Expr. Purif. 17 (1999) 260-266.
6. P. Cramer, D.A. Bushnell, J. Fu, A.L. Gnatt, B. Maier-Davis, N.E. Thompson, R.R. Burgess, A.M. Edwards, P.R. David, R.D. Kornberg, Architecture of RNA polymerase II and implications for the transcription mechanism, Science 288 (2000) 640-649.
7. A.M. Edwards, S.A. Darst, W.J. Feaver, N.E. Thompson, R.R. Burgess, R.D. Kornberg, Purification and lipid-layer crystallization of yeast RNA polymerase II, Proc. Natl. Acad. Sci. USA 87 (1990) 2122-2126.
8. M.J. Breyer, N.E. Thompson, R.R. Burgess, Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase, J. Bacteriol. 179 (1997) 1404-1408.