Characterization of Vibrio cholerae O139 belonging to multiple ribotypes and isolated from diarrhoeal patients in Kerala, southern India

Infection, Genetics and Evolution

Volumn 11, Issue 2, 2011, Pages 454-459

  Fazil, M H U Turabe ^a   Bhanumathi, R ^b,d   Pandey, H P ^c   Singh, D V ^a  

^a INSTITUTE OF LIFE SCIENCES   (India)

^b RAJIV GANDHI CENTRE FOR BIOTECHNOLOGY   (India)

^c BANARAS HINDU UNIVERSITY   (India)

^d Patent Office   (India)

Author keywords

CTX ; CtxB; PCR; Pre CTX ; Recombination; RFLP; Ribotypes

Indexed keywords

AMPICILLIN; CEFALEXIN; CEFOTAXIME; CHLORAMPHENICOL; CHOLERA TOXIN; CHOLERA TOXIN B SUBUNIT; CIPROFLOXACIN; COTRIMOXAZOLE; FURAZOLIDONE; GENTAMICIN; HEMOLYSIN; NALIDIXIC ACID; NEOMYCIN; NORFLOXACIN; OUTER MEMBRANE PROTEIN; POLYMYXIN B; REGULATOR PROTEIN; REPRESSOR PROTEIN; STREPTOMYCIN; TETRACYCLINE; VIRULENCE FACTOR;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM ADHERENCE; BACTERIUM ISOLATION; BACTERIUM PILUS; BIOFILM; CONTROLLED STUDY; DIARRHEA; GENE MUTATION; GENETIC CONSERVATION; GENETIC RECOMBINATION; GENETIC VARIABILITY; INDIA; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REGULATOR GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RIBOTYPING; VIBRIO CHOLERAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOFILMS; CHOLERA; CHOLERA TOXIN; DIARRHEA; DISEASE OUTBREAKS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; FECES; GENES, BACTERIAL; GENOTYPE; HUMANS; INDIA; INTERSPERSED REPETITIVE SEQUENCES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RECOMBINATION, GENETIC; RIBOTYPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; VIBRIO CHOLERAE O139;

VIBRIO CHOLERAE;

EID: 79951950381     PISSN: 15671348     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.meegid.2010.12.008     Document Type: Article

References (42)

1. Alam M., Sultana M., Nair G.B., Siddique A.K., Hasan N.A., Sack R.B., Sack D.A., Ahmed K.U., Sadique A., Watanabe H., Grim C.J., Huq A., Colwell R.R. Viable but nonculturable Vibrio cholerae O1 in biofilms in the aquatic environment and their role in cholera transmission. Proc. Natl. Acad. Sci. U.S.A. 2007, 104:17801-17806.
2. Asadulghani M., Ogura Y., Ooka T., Itoh T., Sawaguchi A., Iguchi A., Nakayama K., Hayashi T. The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants. PLoS Pathog. 2009, 5:e1000408.
3. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Short Protocol in Molecular Biology 1995, John Wiley & Sons, New York. third ed.
4. Basu A., Garg P., Datta S., Chakraborty S., Bhattacharya T., Khan A., Ramamurthy S., Bhattacharya S.K., Yamasaki S., Takeda Y., Nair G.B. Vibrio cholerae O139 in Calcutta, 1992-1998: incidence, antibiograms, and genotypes. Emerg. Infect. Dis. 2000, 6:139-147.
5. Bauer A.W., Kirby W.M., Sherris J.C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966, 45:493-496.
6. Bhanumathi R., Sabeena F., Isac S.R., Radhakutty G., Singh D.V. Characterization of a toxigenic Vibrio cholerae O139 strain belonging to a new ribotype and isolated from a diarrheal patient. J. Clin. Microbiol. 2002, 40:4779-4781.
7. Bhanumathi R., Sabeena F., Isac S.R., Shukla B.N., Singh D.V. Molecular characterization of Vibrio cholerae O139 Bengal isolated from water and the aquatic plant Eichhornia crassipes in the river Ganga, Varanasi, India. Appl. Environ. Microbiol. 2003, 69:2389-2394.
8. Bhuiyan N.A., Nusrin S., Alam M., Morita M., Watanabe H., Ramamurthy T., Cravioto A., Nair G.B. Changing genotypes of cholera toxin (CT) of Vibrio cholerae O139 in Bangladesh and description of three new CT genotypes. FEMS Immunol. Med. Microbiol. 2009, 57:136-141.
9. Boyd E.F., Heilpern A.J., Waldor M.K. Molecular analyses of a putative CTXΦ precursor and evidence for independent acquisition of distinct CTXΦs by toxigenic Vibrio cholerae. J. Bacteriol. 2000, 182:5530-5538.
10. Chatterjee S., Ghosh K., Raychoudhuri A., Pan A., Bhattacharya M.K., Mukhopadhyay A.K., Ramamurthy T., Bhattacharya S.K., Nandy R.K. Phenotypic and genotypic traits and epidemiological implication of Vibrio cholerae O1 and O139 strains in India during 2003. J. Med. Microbiol. 2007, 56:824-832.
11. Coline Stine O. Evolutionary relationships of pathogenic clones of Vibrio cholerae. Vibrio cholerae Genomics and Molecular Biology 2008, 169-176. Caister Academic Press, Norfolk, UK. S.M. Faruque, G.B. Nair (Eds.).
12. Colwell R.R., Kaper J., Joseph S.W. Vibrio cholerae, Vibrio parahaemolyticus, and other vibrios: occurrence and distribution in Chesapeake Bay. Science 1977, 198:394-396.
13. Davis B.M., Waldor M.K. Filamentous phages linked to virulence of Vibrio cholerae. Curr. Opin. Microbiol. 2003, 6:35-42.
14. Farfan M., Minana-Galbis D., Fuste M.C., Loren J.G. Allelic diversity and population structure in Vibrio cholerae O139 Bengal based on nucleotide sequence analysis. J. Bacteriol. 2002, 184:1304-1313.
15. Faruque S.M., Ahmed K.M., Abdul Alim A.R., Qadri F., Siddique A.K., Albert M.J. Emergence of a new clone of toxigenic Vibrio cholerae O1 biotype El Tor displacing V. cholerae O139 Bengal in Bangladesh. J. Clin. Microbiol. 1997, 35:624-630.
16. Faruque S.M., Siddique A.K., Saha M.N., Asadulghani, Rahman M.M., Zaman K., Albert M.J., Sack D.A., Sack R.B. Molecular characterization of a new ribotype of Vibrio cholerae O139 Bengal associated with an outbreak of cholera in Bangladesh. J. Clin. Microbiol. 1999, 37:1313-1318.
17. Faruque S.M., Saha M.N., Asadulghani, Bag P.K., Bhadra R.K., Bhattacharya S.K., Sack R.B., Takeda Y., Nair G.B. Genomic diversity among Vibrio cholerae O139 strains isolated in Bangladesh and India between 1992 and 1998. FEMS Microbiol. Lett. 2000, 184:279-284.
18. Faruque S.M., Chowdhury N., Kamruzzaman M., Ahmad Q.S., Faruque A.S., Salam M.A., Ramamurthy T., Nair G.B., Weintraub A., Sack D.A. Reemergence of epidemic Vibrio cholerae O139, Bangladesh. Emerg. Infect. Dis. 2003, 9:1116-1122.
19. Faruque S.M., Sack D.A., Sack R.B., Colwell R.R., Takeda Y., Nair G.B. Emergence and evolution of Vibrio cholerae O139. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:1304-1309.
20. Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 1983, 132:6-13.
21. Garg P., Aydanian A., Smith D., Morris J.G., Nair G.B., Stine O.C. Molecular epidemiology of O139 Vibrio cholerae: mutation, lateral gene transfer, and founder flush. Emerg. Infect. Dis. 2003, 9:810-814.
22. Kaper J.B., Morris J.G., Levine M.M. Cholera. Clin. Microbiol. Rev. 1995, 8:48-86.
23. Kanungo S., Sah B.K., Lopez A.L., Sung J.S., Paisley A.M., Sur D., Clemens J.D., Nair G.B. Cholera in India: an analysis of reports, 1997-2006. Bull. World Health Organ. 2010, 88:185-191.
24. Lan R., Reeves P.R. Recombination between rRNA operons created most of the ribotype variation observed in the seventh pandemic clone of Vibrio cholerae. Microbiology 1998, 144:1213-1221.
25. Ledon T., Campos J., Suzarte E., Rodriguez B., Marrero K., Fando R. El Tor and Calcutta CTXΦ precursors coexisting with intact CTXΦ copies in Vibrio cholerae O139 isolates. Res. Microbiol. 2008, 159:81-87.
26. Mantri C.K., Mohapatra S.M., Colwell R.R., Singh D.V. Sequence analysis of Vibrio cholerae orfU and zot from pre-CTXF{cyrillic} and CTXΦ reveals multiple origin of pre-CTXF{cyrillic} and CTXΦ. Environ. Microbiol. Rep. 2010, 2:67-75.
27. Mantri C.K., Mohapatra S.M., Singh D.V. Effect of storage and sodium chloride on excision of CTXΦ or pre-CTXΦ and CTXΦ from Vibrio cholerae O139 strains. Infect. Gen. Evol. 2010, 10:925-930.
28. Mekalanos J.J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell 1983, 35:253-263.
29. Nair G.B., Qadri F., Holmgren J., Svennerholm A.M., Safa A., Bhuiyan N.A., Ahmad Q.S., Faruque S.M., Faruque A.S., Takeda Y., Sack D.A. Cholera due to altered El Tor strains of Vibrio cholerae O1 in Bangladesh. J. Clin. Microbiol. 2006, 44:4211-4213.
30. Nusrin S., Khan G.Y., Bhuiyan N.A., Ansaruzzaman M., Hossain M.A., Safa A., Khan R., Faruque S.M., Sack D.A., Hamabata T., Takeda Y., Nair G.B. Diverse CTX phages among toxigenic Vibrio cholerae O1 and O139 strains isolated between 1994 and 2002 in an area where cholera is endemic in Bangladesh. J. Clin. Microbiol. 2004, 42:5854-5856.
31. O'Toole G.A., Pratt L.A., Watnick P.I., Newman D.K., Weaver V.B., Kolter R. Genetic approaches to study of biofilms. Methods Enzymol. 1999, 310:91-109.
32. Ramachandran D., Bhanumathi R., Singh D.V. Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae. J. Med. Microbiol. 2007, 56:346-351.
33. Ramamurthy T., Garg S., Sharma R., Bhattacharya S.K., Nair G.B., Shimada T., Takeda T., Karasawa T., Kurazano H., Pal A., et al. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet 1993, 341:703-704.
34. Safa A., Sultana J., Cam P.D., Mwansa J.C., Kong R.Y. Vibrio cholerae O1 hybrid El Tor strains, Asia and Africa. Emerg. Infect. Dis. 2008, 14:987-988.
35. Sinha S., Chakraborty R., De K., Khan A., Datta S., Ramamurthy T., Bhattacharya S.K., Takeda Y., Nair G.B. Escalating association of Vibrio cholerae O139 with cholera outbreaks in India. J. Clin. Microbiol. 2002, 40:2635-2637.
36. Singh D.V., Isac S.R., Colwell R.R. Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus. J. Clin. Microbiol. 2002, 40:4321-4324.
37. Singh D.V., Matte M.H., Matte G.R., Jiang S., Sabeena F., Shukla B.N., Sanyal S.C., Huq A., Colwell R.R. Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: clonal relationships between clinical and environmental isolates. Appl. Environ. Microbiol. 2001, 67:910-921.
38. Sharma C., Maiti S., Mukhopadhyay A.K., Basu A., Basu I., Nair G.B., Mukhopadhyaya R., Das B., Kar S., Ghosh R.K., Ghosh A. Unique organization of the CTX genetic element in Vibrio cholerae O139 strains which reemerged in Calcatta, India, in September 1996. J. Clin. Microbiol. 1997, 35:3348-3350.
39. Southern E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 1975, 98:503-517.
40. Svennerholm A.M., Holmgren J. Identification of Escherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1-ELISA). Curr. Microbiol. 1978, 1:19-23.
41. Waldor M.K., Mekalanos J.J. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 1996, 272:1910-1914.
42. Waldor M.K., Tschape H., Mekalanos J.J. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J. Bacteriol. 1996, 178:4157-4165.