Identification of a cDNA encoding for Ghrelin in the testis of the frog Pelophylax esculentus and its involvement in spermatogenesis

Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology

Volumn 158, Issue 4, 2011, Pages 367-373

  Izzo, Gaia ^a   Ferrara, Diana ^a   Napolitano, Francesco ^a   Crispo, Alessia Anna ^a   d'Istria, Michela ^a   Aniello, Francesco ^b   Minucci, Sergio ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Ghrelin expression; P. esculentus; Sex hormones; Spermatogenesis; Testis

Indexed keywords

COMPLEMENTARY DNA; GHRELIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FROG; GENE EXPRESSION; GENE IDENTIFICATION; GENE LOCATION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HISTOPATHOLOGY; IN SITU HYBRIDIZATION; MALE; MALE FERTILITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PELOPHYLAX ESCULENTUS; PHYLOGENY; REPRODUCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATOGENESIS; STEROIDOGENESIS; TESTIS;

AMPHIBIA; ANIMALIA; ANURA; MAMMALIA; PELOPHYLAX; VERTEBRATA;

EID: 79951773933     PISSN: 10956433     EISSN: 15314332     Source Type: Journal    
DOI: 10.1016/j.cbpa.2010.09.005     Document Type: Article

References (43)

1. Abou Heif H.M., Deif M.M., Abdel Aziz H.K. Effect of food restriction on ghrelin in adult male rats and its relation to male reproductive hormones. Andrologia 2010, 42:97-105.
2. Barreiro M.L., Gaytán F., Caminos J.E., Pinilla L., Casanueva F.F., Aguilar E., Diéguez C., Tena-Sempere M. Cellular location and hormonal regulation of ghrelin expression in rat testis. Biol. Reprod. 2002, 67:1768-1776.
3. Casanueva F.F., Dieguez C. Ghrelin: the link connecting growth with metabolism and energy homeostasis. Rev. Endocr. Metab. Disord. 2002, 3(4):325-338.
4. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987, 162:156-159.
5. Cobellis G., Pierantoni R., Minucci S., Pernas-Alonso R., Meccariello R., Fasano S. c-fos activity in Rana esculenta testis: seasonal and estradiol-induced changes. Endocrinology 1999, 140:3238-3244.
6. de Rienzo G., Aniello F., Branno M., Minucci S. Isolation and characterization of a novel member of the relaxin/insulin family from the testis of the frog Rana esculenta. Endocrinology 2001, 142:3231-3238.
7. De Rienzo G., Aniello F., Branno M., Izzo G., Minucci S. The expression level of frog relaxin mRNA (fRLX), in the testis of Rana esculenta, is influenced by testosterone. J. Exp. Biol. 2006, 209:3806-3811.
8. Fasano S., Pierantoni R., Minucci S., Di Matteo L., Chieffi G. Seasonal fluctuations of estrogen-binding activity in the testis of the frog, Rana esculenta. Gen. Comp. Endocrinol. 1989, 75(1):157-161.
9. Fernández-Fernández R., Tena-Sempere M., Aguilar E., Pinilla L. Ghrelin effects on gonadotropin secretion in male and female rats. Neurosci. Lett. 2004, 362(2):103-107.
10. Galas L., Chartrel N., Kojima M., Kangawa K., Vaudry H. Immunohistochemical localization and biochemical characterization of ghrelin in the brain and stomach of the frog Rana esculenta. J. Comp. Neurol. 2002, 450:34-44.
11. Gaytan F., Barreiro M.L., Caminos J.E., Chopin L.K., Herington A.C., Morales C., Pinilla L., Paniagua R., Nistal M., Casanueva F.F., et al. Expression of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in normal human testis and testicular tumors. J. Clin. Endocrinol. Metab. 2004, 89:400-409.
12. Grinspoon S., Miller K.K., Herzog D.B., Grieco K.A., Klibanski A. Effects of estrogen and recombinant human insulin-like growth factor-I on ghrelin secretion in severe undernutrition. J. Clin. Endocrinol. Metab. 2004, 89(8):3988-3993.
13. Ishida Y., Sakahara S., Tsutsui C., Kaiya H., Sakata I., Oda S., Sakai T. Identification of ghrelin in the house musk shrew (Suncus murinus): cDNA cloning, peptide purification and tissue distribution. Peptides 2009, 30:982-990.
14. Kaiya H., Kojima M., Hosoda H., Koda A., Yamamoto K., Kitajima Y., Matsumoto M., Minamitake Y., Kikuyama S., Kangawa K. Bullfrog ghrelin is modified by n-octanoic acid at its third threonine residue. J. Biol. Chem. 2001, 276(44):40441-40448.
15. Kaiya H., Takagi T., Yamasaki I., Denbow D.M., Kangawa K., Furuse M., Saito E.S. Chicken ghrelin and growth hormone-releasing peptide-2 inhibit food intake of neonatal chicks. Eur. J. Pharmacol. 2002, 453(1):75-79.
16. Kaiya H., Kojima M., Hosoda H., Moriyama S., Takahashi A., Kawauchi H., Kangawa K. Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout. Endocrinology 2003, 144(12):5215-5226.
17. Kaiya H., Kojima M., Hosoda H., Riley L.G., Hirano T., Grau E.G., Kangawa K. Amidated fish ghrelin: purification, cDNA cloning in the Japanese eel and its biological activity. J. Endocrinol. 2003, 176(3):415-423.
18. Kaiya H., Kojima M., Hosoda H., Riley L.G., Hirano T., Grau E.G., Kangawa K. Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2003, 135(3):421-429.
19. Kaiya H., Sakata I., Kojima M., Hosoda H., Sakai T., Kangawa K. Structural determination and histochemical localization of ghrelin in the red-eared slider turtle, Trachemys scripta elegans. Gen. Comp. Endocrinol. 2004, 138(1):50-57.
20. Kaiya H., Miyazato M., Kangawa K., Peter R.E., Unniappan S. Ghrelin: a multifunctional hormone in non-mammalian vertebrates. Comp. Biochem. Physiol. A 2008, 149:109-128.
21. Kellokoski E., Pöykkö S.M., Karjalainen A.H., Ukkola O., Heikkinen J., Kesäniemi Y.A., Hörkkö S. Estrogen replacement therapy increases plasma ghrelin levels. J. Clin. Endocrinol. Metab. 2005, 90(5):2954-2963.
22. Kheradmand A., Roshangar L., Taati M. The role of ghrelin on the morphometry and intracellular changes in the rat testis. Tissue Cell 2009, 41(2):105-111.
23. Kojima M., Hosoda H., Date Y., Nakazato M., Matsuo H., Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999, 402:656-660.
24. Kojima M., Hosoda H., Kangawa K. Purification and distribution of ghrelin: the natural endogenous ligand for the growth hormone secretagogue receptor. Horm. Res. 2001, 56(Suppl 1):93-97.
25. Lebenthal Y., Gat-Yablonski G., Shtaif B., Padoa A., Phillip M., Lazar L. Effect of sex hormone administration on circulating ghrelin levels in peripubertal children. J. Clin. Endocrinol. Metab. 2006, 91(1):328-331.
26. Lorenzi T., Meli R., Marzioni D., Morroni M., Baragli A., Castellucci M., Gualillo O., Muccioli G. Ghrelin: a metabolic signal affecting the reproductive system. Cytokine Growth Factor Rev. 2009, 20(2):137-152.
27. Martini A.C., Fernández-Fernández R., Tovar S., Navarro V.M., Vigo E., Vazquez M.J., Davies J.S., Thompson N.M., Aguilar E., Pinilla L., et al. Comparative analysis of the effects of ghrelin and unacylated ghrelin on luteinizing hormone secretion in male rats. Endocrinology 2006, 147(5):2374-2382.
28. Minucci S., Di Matteo L., Fasano S., Baccari G.C., Pierantoni R. Intratesticular control of spermatogenesis in the frog, Rana esculenta. J. Exp. Zool. 1992, 264(1):113-118.
29. Morash M.G., Gagnon J., Nelson S., Anini Y. Tissue distribution and effects of fasting and obesity on the ghrelin axis in mice. Regul. Pept. 2010, (Epub ahead of print).
30. Murashita K., Kurokawa T., Nilsen T.O., Rønnestad I. Ghrelin, cholecystokinin, and peptide YY in Atlantic salmon (Salmo salar): molecular cloning and tissue expression. Gen. Comp. Endocrinol. 2009, 160(3):223-235.
31. Pagotto U., Gambineri A., Pelusi C., Genghini S., Cacciari M., Otto B., Castañeda T., Tschöp M., Pasquali R. Testosterone replacement therapy restores normal ghrelin in hypogonadal men. J. Clin. Endocrinol. Metab. 2003, 88(9):4139-4143.
32. Palmiero C., Ferrara D., De Rienzo G., d'Istria M., Minucci S. Ethane 1, 2-dimethane sulphonate is a useful tool for studying cell-to-cell interactions in the testis of the frog, Rana esculenta. Gen. Comp. Endocrinol. 2003, 131:38-47.
33. Parhar I.S., Sato H., Sakuma Y. Ghrelin gene in cichlid fish is modulated by sex and development. Biochem. Biophys. Res. Commun. 2003, 305:169-175.
34. Sakata I., Tanaka T., Matsubara M., Yamazaki M., Tani S., Hayashi Y., Kangawa K., Sakai T. Postnatal changes in ghrelin mRNA expression and in ghrelin-producing cells in the rat stomach. J. Endocrinol. 2002, 174:463-471.
35. Sanger F., Coulson A.R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 1975, 94:441-448.
36. Sirotkin A.V., Chrenkova M., Nitrayova S., Patras P., Darlak K., Valenzuela F., Pinilla L., Tena-Sempere M. Effects of chronic food restriction and treatments with leptin or ghrelin on different reproductive parameters of male rats. Peptides 2008, 29:1362-1368.
37. Svechnikov K., Landreh L., Weisser J., Izzo G., Colón E., Svechnikova I., Söder O. Origin, development and regulation of human Leydig cells. Horm. Res. Paediatr. 2010, 73:93-101.
38. Tanaka M., Hayashida Y., Nakao N., Nakai N., Nakashima K. Testis-specific and developmentally induced expression of a ghrelin gene-derived transcript that encodes a novel polypeptide in the mouse. Biochim. Biophys. Acta 2001, 1522:62-65.
39. Tena-Sempere M. Ghrelin: novel regulator of gonadal function. J. Endocrinol. Invest. 2005, 28(5 Suppl):26-29. Review.
40. Tena-Sempere M., Barreiro M.L., González L.C., Gaytán F., Zhang F.P., Caminos J.E., Pinilla L., Casanueva F.F., Diéguez C., Aguilar E. Novel expression and functional role of ghrelin in rat testis. Endocrinology 2002, 143:717-725.
41. Unniappan S. Ghrelin: an emerging player in the regulation of reproduction in non-mammalian vertebrates. Gen. Comp. Endocrinol. 2010, 167:340-343.
42. Unniappan S., Lin X., Cervini L., Rivier J., Kaiya H., Kangawa K., Peter R.E. Goldfish ghrelin: molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake. Endocrinology 2002, 143:4143-4146.
43. Varriale B., Pierantoni R., Di Matteo L., Minucci S., Fasano S., D'Antonio M., Chieffi G. Plasma and testicular estradiol and plasma androgen profile in the male frog Rana esculenta during the annual cycle. Gen. Comp. Endocrinol. 1986, 64:401-404.