Continuous On-Line Monitoring of Secretion from Rodent Pituitary Endocrine Cells Using Fluorescent Protein Surrogate Markers

Journal of Neuroendocrinology

Volumn 23, Issue 3, 2011, Pages 197-207

  He, Z ^a   Fernandez Fuente, M ^a   Strom, M ^a   Cheung, L ^a   Robinson, I C ^a   Le Tissier, P ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

Dopamine; Ghrelin; Growth hormone; Growth hormone releasing hormone; Prolactin; Secretion

Indexed keywords

DOPAMINE; GREEN FLUORESCENT PROTEIN; GROWTH HORMONE; GROWTH HORMONE RELEASING FACTOR; HISTIDYL DEXTRO TRYPTOPHYLALANYLTRYPTOPHYL DEXTRO PHENYLALANYLLYSINAMIDE; OXYTOCIN; SOMATOSTATIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL POPULATION; CELL STIMULATION; CONTROLLED STUDY; DISEASE MARKER; ENDOCRINE CELL; EXOCYTOSIS; FEMALE; HORMONE RELEASE; HYPOPHYSIS CELL; IN VIVO STUDY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROLACTIN SECRETING CELL; PROTEIN SECRETION; PROTEIN TARGETING; RADIOIMMUNOASSAY; RAT; RODENT; SECRETORY VESICLE; TISSUE SLICE; TRANSGENE; WILD TYPE;

ANIMALS; BIOLOGICAL MARKERS; CELL TRACKING; DRUG MONITORING; DWARFISM; ENDOCRINE CELLS; FEMALE; GREEN FLUORESCENT PROTEINS; GROWTH HORMONE-RELEASING HORMONE; MALE; MICE; MICE, INBRED C57BL; MICE, INBRED CBA; MICE, TRANSGENIC; OLIGOPEPTIDES; ONLINE SYSTEMS; PERFUSION; RATS; RATS, TRANSGENIC; SOMATOSTATIN; SOMATOTROPHS;

EID: 79951715795     PISSN: 09538194     EISSN: 13652826     Source Type: Journal    
DOI: 10.1111/j.1365-2826.2010.02104.x     Document Type: Article

References (38)

1. Ling N, Ying SY, Ueno N, Esch F, Denoroy L, Guillemin R. Isolation and partial characterization of a Mr 32, 000 protein with inhibin activity from porcine follicular fluid. Proc Natl Acad Sci USA 1985; 82: 7217-7221.
2. Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier J, Guillemin R. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 1973; 179: 77-79.
3. Gillies GE, Linton EA, Lowry PJ. Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin. Nature 1982; 299: 355-357.
4. Christian HC, Chapman LP, Morris JF. Thyrotrophin-releasing hormone, vasoactive intestinal peptide, prolactin-releasing peptide and dopamine regulation of prolactin secretion by different lactotroph morphological subtypes in the rat. J Neuroendocrinol 2007; 19: 605-613.
5. Stachura ME, Tyler JM, Kent PG. Pituitary immediate release pools of growth hormone and prolactin are preferentially refilled by new rather than stored hormone. Endocrinology 1989; 125: 444-449.
6. Hoeffler JP, Frawley LS. Capacity of individual somatotropes to release growth hormone varies according to sex: analysis by reverse hemolytic plaque assay. Endocrinology 1986; 119: 1037-1041.
7. Bonnefont X, Lacampagne A, Sanchez-Hormigo A, Fino E, Creff A, Mathieu MN, Smallwood S, Carmignac D, Fontanaud P, Travo P, Alonso G, Courtois-Coutry N, Pincus SM, Robinson IC, Mollard P. Revealing the large-scale network organization of growth hormone-secreting cells. Proc Natl Acad Sci USA 2005; 102: 16880-16885.
8. Steyer JA, Almers W. A real-time view of life within 100nm of the plasma membrane. Nat Rev Mol Cell Biol 2001; 2: 268-275.
9. Nunes P, Hasler U, McKee M, Lu HA, Bouley R, Brown D. A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2. Am J Physiol Cell Physiol 2008; 295: C1476-C1487.
10. Magoulas C, McGuinness L, Balthasar N, Carmignac DF, Sesay AK, Mathers KE, Christian H, Candeil L, Bonnefont X, Mollard P, Robinson IC. A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice. Endocrinology 2000; 141: 4681-4689.
11. Charlton HM, Clark RG, Robinson IC, Goff AE, Cox BS, Bugnon C, Bloch BA. Growth hormone-deficient dwarfism in the rat: a new mutation. J Endocrinol 1988; 119: 51-58.
12. Jones BK, Monks BR, Liebhaber SA, Cooke NE. The human growth hormone gene is regulated by a multicomponent locus control region. Mol Cell Biol 1995; 15: 7010-7021.
13. Flavell DM, Wells T, Wells SE, Carmignac DF, Thomas GB, Robinson ICAF. Dominant dwarfism in transgenic rats by targeting human growth hormone (GH) expression to hypothalamic GH-releasing factor neurons. EMBO J 1996; 15: 3871-3879.
14. Castrique E, Fernandez-Fuente M, Le Tissier P, Herman A, Levy A. Use of a prolactin-Cre/ROSA-YFP transgenic mouse provides no evidence for lactotroph transdifferentiation after weaning, or increase in lactotroph/somatotroph proportion in lactation. J Endocrinol 2010; 205: 49-60.
15. Carmignac DF, Robinson IC. Growth hormone (GH) secretion in the dwarf rat: release, clearance and responsiveness to GH-releasing factor and somatostatin. J Endocrinol 1990; 127: 69-75.
16. Le Tissier PR, Carmignac DF, Lilley S, Sesay AK, Phelps CJ, Houston P, Mathers K, Magoulas C, Ogden D, Robinson IC. Hypothalamic growth hormone-releasing hormone (GHRH) deficiency: targeted ablation of GHRH neurons in mice using a viral ion channel transgene. Mol Endocrinol 2005; 19: 1251-1262.
17. Clark RG, Mortensen DL, Carlsson LM, Carlsson B, Carmignac D, Robinson IC. The obese growth hormone (GH)-deficient dwarf rat: body fat responses to patterned delivery of GH and insulin-like growth factor-I. Endocrinology 1996; 137: 1904-1912.
18. Duncan RR, Greaves J, Wiegand UK, Matskevich I, Bodammer G, Apps DK, Shipston MJ, Chow RH. Functional and spatial segregation of secretory vesicle pools according to vesicle age. Nature 2003; 422: 176-180.
19. 2+ and insulin secretory responses of MIN6 pseudoislets. Diabetes 1999; 48: 1402-1408.
20. Balthasar N, Mery PF, Magoulas CB, Mathers KE, Martin A, Mollard P, Robinson ICAF. Growth hormone-releasing hormone (GHRH) neurons in GHRH-enhanced green fluorescent protein transgenic mice: a ventral hypothalamic network. Endocrinology 2003; 144: 2728-2740.
21. Ueta Y, Fujihara H, Serino R, Dayanithi G, Ozawa H, Matsuda K, Kawata M, Yamada J, Ueno S, Fukuda A, Murphy D. Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat. Endocrinology 2005; 146: 406-413.
22. Katoh A, Fujihara H, Ohbuchi T, Onaka T, Young WS III, Dayanithi G, Yamasaki Y, Kawata M, Suzuki H, Otsubo H, Murphy D, Ueta Y. Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary. J Endocrinol 2010; 204: 275-285.
23. Cheng K, Chan WW, Butler B, Wei L, Schoen WR, Wyvratt MJ Jr, Fisher MH, Smith RG. Stimulation of growth hormone release from rat primary pituitary cells by L-692, 429, a novel non-peptidyl GH secretagogue. Endocrinology 1993; 132: 2729-2731.
24. Clark RG, Carlsson LMS, Trojnar J, Robinson ICAF. The effects of a growth hormone-releasing peptide and growth hormone releasing factor in conscious and anaesthetized rats. J Neuroendocrinol 1989; 1: 249-255.
25. Carmignac DF, Bennett PA, Robinson ICAF. Effects of growth hormone secretagogues on prolactin release in anesthetized dwarf (dw/dw) rats. Endocrinology 1998; 139: 3590-3596.
26. Bowers CY, Reynolds GA, Durham D, Barrera CM, Pezzoli SS, Thorner MO. Growth hormone (GH)-releasing peptide stimulates GH release in normal men and acts synergistically with GH-releasing hormone. J Clin Endocrinol Metab 1990; 70: 975-982.
27. Guillemin R, Brazeau P, Bohlen P, Esch F, Ling N, Wehrenberg WB, Bloch B, Mougin C, Zeytin F, Baird A. Somatocrinin, the growth hormone releasing factor. Recent Prog Horm Res 1984; 40: 233-299.
28. Giustina A, Veldhuis JD. Pathophysiology of the neuroregulation of growth hormone secretion in experimental animals and the human. Endocr Rev 1998; 19: 717-797.
29. Tierney T, Robinson IC. Increased lactotrophs despite decreased somatotrophs in the dwarf (dw/dw) rat: a defect in the regulation of lactotroph/somatotroph cell fate? J Endocrinol 2002; 175: 435-446.
30. Downs TR, Frohman LA, Kineman RD, Aleppo G. Evidence for a defect in growth hormone-releasing factor signal transduction in the dwarf (dw/dw) rat pituitary. Endocrinology 1991; 129: 58-67.
31. Howard AD, Feighner SD, Cully DF, Arena JP, Liberator CI, Hamelin M, Hreniuk DL, Palyha OC, Anderson J, Paress PS, Diaz C, Chou M, Liu KK, McKee KK, Pong SS, Chaung LY, Elbrecht M, Heavens R, Rigby M, Sirinathsinghji DJS, Dean DC, Melillo DG, Patchett AA, Nargund R, Griffin PR, DeMartino JA, Gupta SK, Schaeffer JM, Smith RG, VanderPloeg LHT. A receptor in pituitary and hypothalamus that functions in growth hormone release. Science 1996; 273: 974-977.
32. Godfrey P, Rahal J, Beamer W, Copeland N, Jenkins N, Mayo K. GHRH receptor of little mice contains a missense mutation in the extracellular domain that disrupts receptor function. Nat Genet 1993; 4: 227-232.
33. Cheng K, Chan WWS, Barreto A, Convey EM, Smith RG. The synergistic effects of His-D-Trp-Ala-Trp-D-Phe-Lys-Nh2. Endocrinology 1989; 124: 2791-2798.
34. Ben-Jonathan N, Hnasko R. Dopamine as a prolactin (PRL) inhibitor. Endocr Rev 2001; 22: 724-763.
35. Denef C, Baes M, Schramme C. Stimulation of prolactin secretion after short term or pulsatile exposure to dopamine in superfused anterior pituitary cell aggregates. Endocrinology 1984; 114: 1371-1378.
36. Freeman ME, Kanyicska B, Lerant A, Nagy G. Prolactin: structure, function, and regulation of secretion. Physiol Rev 2000; 80: 1523-1631.
37. Munaka T, Abe H, Kanai M, Sakamoto T, Nakanishi H, Yamaoka T, Shoji S, Murakami A. Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis. Anal Biochem 2006; 353: 1-6.
38. Roper MG, Shackman JG, Dahlgren GM, Kennedy RT. Microfluidic chip for continuous monitoring of hormone secretion from live cells using an electrophoresis-based immunoassay. Anal Chem 2003; 75: 4711-4717.