A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice

Endocrinology

Volumn 141, Issue 12, 2000, Pages 4681-4689

  Magoulas, C ^a   Mcguinness, L ^a   Balthasar, N ^a   Carmignac, D F ^a   Sesay, A K ^a   Mathers, K E ^a   Christian, H ^b   Candeil, L ^c   Bonnefont, X ^c   Mollard, P ^c   Robinson, I C A F ^a,d  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c INSERM   (France)

^d QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; GROWTH HORMONE; SIGNAL PEPTIDE;

ADENOHYPOPHYSIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CALCIUM CELL LEVEL; CELL ISOLATION; CONTROLLED STUDY; COSMID; FLUORESCENCE; FLUORESCENCE ACTIVATED CELL SORTER; GENE LOCUS; HYPOPHYSIS CELL; MEMBRANE VESICLE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SECRETION; TRANSGENE; TRANSGENIC MOUSE;

EID: 17744395514     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.141.12.7828     Document Type: Article

References (45)

1. The importance of the secretory pattern of growth hormone for statural growth
2. Control of growth hormone (GH) release by GH secretagogueues
3. The green fluorescent protein
4. Expression of green fluorescent protein in the ureteric bud of transgenic mice: A new tool for the analysis of ureteric bud morphogenesis
5. Green fluorescent protein as a reporter of gene expression in transgenic mice
6. Transgernic expression of green fluorescent protein in mouse oxytocin neurones
7. GABA- and glutamate-activated channels in green fluorescent protein-tagged gonadotropin-releasing hormone neurons in transgenic mice
8. A transgenic mouse model to analyze CD8(+) effector T cell differentiation in vivo
9. Green fluorescent protein-transgenic mice: Immune functions and their application to studies of lymphocyte development
10. Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras
11. Tracking single secretory granules in live chromaffin cells by evanescent-field fluorescence microscopy
12. Transport, docking and exocytosis of single secretory granules in live chromaffin cells
13. Synchronized spontaneous Ca2+ transients in acute anterior pituitary slices
14. Targeting fluorescent reporters to the hypothalamo-pituitary G.H. axis in transgenic mice
15. The human growth hormone gene is regulated by a multicomponent locus control region
16. Dominant dwarfism in transgenic rats by targeting human growth hormone (GH) expression to hypothalamic GH-releasing factor neurons
17. Intrahypothalamic growth hormone feedback: From dwarfism to acromegaly in the rat
18. Growth hormone (GH) secretion in the dwarf rat: Release, clearance and responsiveness to GH-releasing factor and somatostatin
19. The development and evaluation of a sensitive and specific radioimmunoassay for oxytocin in unextracted plasma
20. Rhythmic bursts of calcium transients in acute anterior pituitary slices
21. 'Green mice' as a source of ubiquitous green cells
22. Genetic targeting of green fluorescent protein to gonadotropin-releasing hormone neurons: Characterization of whole-cell electrophysiological properties and morphology
23. A functional DNA binding domain is required for growth hormone-induced nuclear accumulation of Stat5B
24. Membrane targeting and cytoplasmic sequestration in the spatiotemporal localization of human protein kinase C alpha
25. Targeting of green fluorescent protein to neuroendocrine secretory granules: A new tool for real time studies of regulated protein secretion
26. Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 Cells
27. Human growth hormone: Complementary DNA cloning and expression in bacteria
28. Human interleukin 1β fused to the human growth hormone signal peptide is N-glycosylated and secreted by Chinese hamster ovary cells
29. Addition of growth hormone secretion signal to basic fibroblast growth factor results in cell transformation and secretion of aberrant forms of the protein
30. Introns increase transcriptional efficiency in transgenic mice
31. Dimerization of human growth hormone by zinc
32. DNase I-hypersensitive sites I and II of the human growth hormone locus control region are a major developmental activator of somatotrope gene expression
33. Pit-1 binding sites at the somatotrope-specific DNase I hypersensitive sites I, II of the human growth hormone locus control region are essential for in vivo hGH-N gene activation
34. Physical linkage of the human growth hormone gene cluster and the CD79b (Ig β/B29) gene
35. Expression of truncated forms of the bovine growth hormone gene in cultured mouse cells
36. Mutations in the third α-helix of bovine growth hormone dramatically affect its intracellular distribution in vitro and growth enhancement in transgenic mice
37. Synthesis of growth hormone-prolactin chimeric proteins and processing mutants by the exchange and deletion of genomic exons
38. Re-routing of a secretory protein by fusion with human growth hormone sequences
39. Purification of functional lactotrophs and somatotrophs from female rats using fluorescence-activated cell sorting
40. Flow cytometric analysis of functional anterior pituitary cells from female rats
41. 2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon
42. Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes
43. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins