Cloning and expression of a single human immunoglobulin heavy-chain variable domain with vascular endothelial growth factor binding activity

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 26, Issue 11, 2010, Pages 1555-1562

  Liu, Heng ^a,b   Liu, Siguo ^b   Wu, Yi ^b   Ma, Zili ^a,b   Liu, Yu ^c   Zhang, Aimin ^b   Chen, Jianquan ^b   Cheng, Guoxiang ^b  

^a TONGJI UNIVERSITY   (China)

^b Shanghai Transgenic Research Center   (China)

^c CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Heavy chain antibody; Human antibody; SdAbs; Vascular endothelial growth factor

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; VASCULOTROPIN A;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

ESCHERICHIA COLI; MUS;

EID: 79851480997     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med, 1995, 1(1): 27-31.
2. Dimitrov DS, Marks JD. Therapeutic antibodies: current state and future trends-is a paradigm change coming soon? Methods Mol Biol, 2009, 525: 1-27.
3. Carter P, Merchant AM. Engineering antibodies for imaging and therapy. Curr Opin Biotechnol, 1997, 8: 449-454.
4. Wesolowski J, Alzogaray V, Reyelt J, et al. Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. Med Microbiol Immunol, 2009, 198(3): 157-74.
5. Liu Y, Yun DJ, Chen JQ, et al. Isolation and characterization of human anti-VEGF165 monoclonal antibody with anti-tumor efficacy from transgenic mice expressing human immunoglobulin loci. Cancer Lett, 2009, 273: 28-34.
6. Goding JW. Monoclonal Antibodies: Principles and Practice. 3rd ed. London: Academic Press, 1988: 43-434.
7. Clark MF, Adams AN. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol, 1977, 34: 475-483.
8. Schroeder HW Jr, Hillson JL, Perlmutter RM. Early restriction of the human antibody repertoire. Science, 1987, 238: 791-793.
9. Ward ES, Gussow D, Grifiths AD, et al. Binding activities of a repertoire of single immunoglobulin variable domains secreted from E. coli. Nature, 1989, 341: 544-546.
10. Riechmann L, Muyldermans S. Single domain antibodies: comparison of camel VH and camelised human VH domains. J Immunol Methods, 1999, 231: 25-38.
11. Heap CJ, Wang Y, Pinheiro TJT, et al. Analysis of a 17-amino acid residue, virus-neutralizing microantibody. J Gen Virol, 2005, 86: 1791-1800.
12. Ho M, Kreitman RJ, Onda M, et al. In vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin. J Biol Chem, 2005, 280(1): 607-617.
13. Qiu XQ, Wang H, Cai B, et al. Small antibody mimetics comprising two complementarity-determining regions and a framework region for tumor targeting. Nat Biotechnol, 2007, 25(8): 921-929.
14. Nicholson IC, Zou X, Popov AV, et al. Antibody repertoires of four and five feature translocus mice carrying human immunoglobulin heavy chain and kappa and lambda light chain yeast artificial chromosomes. J Immunol, 1999, 163: 6898-6906.
15. Ren J, Bai J, Si XM, et al. Effect of disproportional expression of the aberrant and correct kappa chain transcripts on antibody secretion of SP2/0 derived-CEA and AFP hybridomas. Int J Mod Cancer Ther, 1999, 2(2): 65-70.
16. Carlson LM, McCormack WT, Postema CE, et al. Templated insertions in the rearranged chicken IgL V gene segment arise by intrachromosomal gene conversion. Genes Dev, 1990, 4: 536-547.