Real time PCR based on fluorescent quenching of mercaptoacetic acid-modified cdte quantum dots for ultrasensitive specific detection of nucleic acids

Nano Biomedicine and Engineering

Volumn 2, Issue 1, 2010, Pages 45-55

  Cui, Daxiang ^a   Li, Qing ^a   Huang, Peng ^a   Wang, Kan ^a   Kong, Yifei ^a   Zhang, Hong ^b   You, Xiaogang ^a   He, Rong ^a   Song, Hua ^a   Wang, Jingping ^b   Bao, Chenchen ^a   Asahi, Toru ^b   Gao, Feng ^a   Osaka, Tetsuya ^b  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b WASEDA UNIVERSITY   (Japan)

Author keywords

Mercaptoacetic acid modified cdte quantum dot; Photoluminescence; Polymerase chain reaction; Sensitivity; Specificity

Indexed keywords

EID: 79551666907     PISSN: None     EISSN: 21505578     Source Type: Journal    
DOI: 10.5101/nbe.v2i1.p44-54     Document Type: Article

References (45)

1. Jeong H, Chang AM, Melloch MR, The Kondo effect in an artificial quantum dot molecule. Science 2001; 293: 2221-2223
2. Cui D, Han Y, Li Z, Song H, Wang K, He R, et al. Fluorescent magnetic nanoprobes for in vivo targeted imaging and hyperthermia therapy of prostatecancer. Nano Biomed Eng 2009; 1(1): 94-112.
3. Li Z, Huang P, He R, Lin J, Yang S, Zhang X, et al. Aptamer-conjugated dendrimer-modified quantum dots for cancer cell targeting and imaging. Mater. Lett. 2010; 64: 375-378
4. Lu, W, Ji Z Q, Pfeiffer, L, West, K W, Rimberg, A J. Real-time detection of electron tunnelling in a quantum dot. Nature 2003; 423: 422-425
5. Kaul, Z, Yaguchi T, Kaul SC Hirano, T Wadhwa R, Taira K. Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates. Cell Res. 2003; 13: 503-507
6. Medintz IL, Uyeda HT, Goldman ER, Mattoussi H. Quantum dot bioconjugates for imaging, labelling and sensing. Nature Mater. 2005; 4: 435-446
7. Yang H, Guo Q, He R, Li D, Zhang XQ, Bao CC, Hu HY, Cui DX. A quick and parallel analytical method based on quantum dots labeling for ToRCH-related antibodies. Nanoscale Res. Lett. 2009; 4: 1469-1474
8. Cui DX, Pan BF, Zhang H, et al. Self-assembly of CNTs and Quantum dots for ultrasensitive DNA and antigen detction. Analytical Chemsitry, 2008, 80(21)7996-8001
9. Han M, Gao X, Su J Z, Nie SM. Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nature Biotechnol. 2001; 19: 631-635
10. Jares-Erijman E A, Jovin T M. FRET imaging. Nature Biotechnology 2003; 21: 1387-1395
11. Huang X Y, Li L, Qian H F, Dong C Q, Ren C J. A resonance energy transfer between chemiluminescent donors and luminescent quantum-dots as acceptors (CRET). Angew. Chem. Int. Ed. 2006; 45: 5140-5143.
12. Alivisato P. The use of nanocrystals in biological detection. Nature Biotechnol. 2004; 22: 47-52
13. Dong CQ, Qian HF, Fang NH, Ren JC. Study of fluorescence quenching and dialysis process of CdTe quantum dots, using ensemble techniques and fluorescence correlation spectroscopy. J. Phys. Chem. B. 2006; 110: 11069-11075
14. Ullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987; 155: 335-350
15. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonak J, Lind K, Sindelka R, Sjoback R, Sjogreen B, Strombom L, Stahlberg A, Zoric N. The real-time polymerase chain reaction. Mol. Aspects Med. 2006; 27: 95-125
16. Chen F, Binder B, Hodson RE. Flow cytometric detection of specific gene expression in prokaryotic cells using in situ RT-PCR. FEMS Microbiol. 2000; 184: 291-295.
17. Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA-sequences. Biotechnology 1992; 10:423-417.
18. Lagally E T, Simpson PC, Mathies R A. Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system. Sensors Actuators B 2000; 63: 38-146
19. Cui D, Tian F R, Kong Y, Igor T, Gao H. Effects of Single wall carbon nnaotubes on polymerase chain reaction. Nanotechnology 2004;15:154-157
20. Shen CC, Yang W J, Ji Q L, Maki H, Dong A J, Zhang Z Z. NanoPCR observation: different levels of DNA replication fidelity in nanoparticle enhanced polymerase chain reactions. Nanotechnology 2009; 20: 455103.
21. Li Q, He R, Gao F, Cui, D. The Effect s of mercaptoacetic acid modified CdTe quantum dot s on specificity of polymerase chain reaction. Journal of Shanghai Jiaotong university 2008; 5: 693-696.
22. Li H, Huang J, Lv J, An H, Zhang X, Zhang Z, Fan C, Hu J. Nanoparticle PCR: Nanogold-assisted PCR with enhanced specificity. Angew. Chem. Int. Ed. 2005; 44: 5100-5103.
23. Li M, Lin YC, Wu C C, Liu H S. Enhancing the efficiency of a PCR using gold nanoparticles. Nucleic Acids Research. 2005; 33: e184
24. Yang W C, Mi L, Cao X Y, Zhang X D, Fan C H, Hu J. Evaluation of gold nanoparticles as the additive in real-time polymerase chain reaction with SYBR Green I dye. Nanotechnology 2008; 19: 255101
25. Cui D, Jin G, Gao T W, Sun T, Tian F, Estrada G G, Gao H, Sarai A. Characterization of BRCAA1 and its novel epitope identification. Cancer Epidemiol Biomarkers Prev. 2004; 13: 1136-1145.
26. He R, You X, Shao J, Gao F, Pan B, Cui D. Core/shell fluorescent magnetic silica-coated composite nanoparticles for bioconjugation. Nanotechnology.2007; 18 (31):315601.
27. Mamedova N N, Kotov NA. Albumin-CdTe Nanoparticle Bioconjugates: Preparation, Structure, and Interunit Energy Transfer with Antenna effect. Nano Lett. 2001; 1: 281-286.
28. Asuri P, Bale SS, Karajanagi S S, Kane R S. The protein-nanomaterial interface. Current opinion in biotechnology 2006; 17: 562-568
29. Ponchel F, Toomes C, Bransfield K, Leong F T, Douglas S H, Field S L, Bell S M, Combaret V, Puisieux A, Mighell A J et al. Real time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications, and micro gene delections. BMC Biotechnol. 2003; 3:18-20
30. Wang Y M, Zhu W, Levy D E. Nuclear and cytoplasmic mRNA quantification by SYBR green based real-time RT-PCR. Methods 2006; 39: 356-362
31. Ji XQ, Sato H, Tanaka H, Konishi Y, Fujimoto T, Takahashi O, Tanaka T. Real-time quantitative RT-PCR detection of disseminated endometrial tumor cells in peripheral blood and lymph nodes using the LightCycler System. Gynecol Oncol. 2006; 100: 355-60.
32. Wright P A, Wynford-Thomas D. The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research. J Pathol.1990; 162: 99-117
33. Talapin D V, Gaponik N, Borchert H, Rogach A L, Haase M, Weller H. Etching of colloidal InP nanocrystals with fluorides: Photochemical nature of the process resulting in high photoluminescence efficiency. J. Phys. Chem. B. 2002; 106: 7177-7180
34. Helot A, Favero I, Cassabois G, Voisin C, Delalande C, Roussignol Ph, Ferreira R, Gerard J M. Unconventional motional narrowing in the optical spectrum of a semiconductor quantum dot. Nature Physics 2006; 2: 759-764
35. Wang P. Nanoscale biocatalyst systems. Current Opinion in biotechnology 2006; 17: 74-579
36. Bigion T P, Lin X M, Nguyen T T, Corwin E I, Witten T A, Jaeger H M. Kinetically driven self assembly of highly ordered nnaoparticle monolayers. Nature Materials 2006; 5: 265-270
37. Eijt S W H, Veen A V, Schut H, Mijnarends P E, Denison A B, Barbiellini B, Bansil A A. Study of colloid quantum-dot surfaces using an innovative thin-film positron 2D-ACAR method. Nature Materials 2006; 5: 23-26.
38. Mitchell G P, Mirkin C A, Lrtsinger R L. Programmed assembly of DNA functionalized quantum dots J. Am. Chem.soc. 1999; 121: 8122-8124
39. Gaponik N, Talapin D V, Rogach A L, Hoppe K, Shevchenko E V, Kornowski A, Eychmuller A, Weller H. Thiol-capping of CdTe nanocrystals: An alternative to organometallic synthetic routes. J. Physics Chem. B 2002; 106: 7177-7185
40. Kenworthy A K. Imaging protein-protein interactions using fluorescence resonance energy transfer microscpy. Methods 2001; 24: 289-296
41. Perales C, Cava F, Meijer W J J, Berenguer J. Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein. Nucleic Acids Res.2003; 31: 6473-6480
42. Lee S H, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H. Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli. J. Biosci. Biotechnol. Biochem. 1994; 58: 1490-1495
43. Jiao G S, Thoresen L H, Kim T G, Haaland W C, Gao F, Topp M R, Hochstrasser R M, Metzker M L, Burgess K. Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology. Chemistry -- A European Journal 2006; 12: 7816-7826.
44. Chou Q. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 1992; 20: 1717-1723.
45. Chou Q. Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB. Nucleic Acids Res. 1992; 20: 4371