Nuclear and cytoplasmic mRNA quantification by SYBR green based real-time RT-PCR

Methods

Volumn 39, Issue 4, 2006, Pages 356-362

  Wang, Yaming ^a   Zhu, Wei ^a   Levy, David E ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cell fractionation; Detergent lysis; Geometric normalization; mRNA export; Quantitative real time PCR

Indexed keywords

MESSENGER RNA;

ACCURACY; ARTICLE; CELL FRACTIONATION; DNA BINDING; GENE AMPLIFICATION; PRIORITY JOURNAL; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; STEADY STATE; TISSUE CULTURE;

CELL NUCLEUS; CYTOPLASM; FLUORESCENT DYES; ORGANIC CHEMICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 33748457358     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2006.06.010     Document Type: Article

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