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Volumn 1, Issue 6, 2001, Pages 281-286

Albumin-CdTe Nanoparticle Bioconjugates: Preparation, Structure, and Interunit Energy Transfer with Antenna Effect

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EID: 0346977251     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl015519n     Document Type: Article
Times cited : (440)

References (52)
  • 27
    • 0347796093 scopus 로고    scopus 로고
    • note
    • 2-:L-cysteine was 1:0.47:2.43. The solution was refluxed for ca. 1 h to promote growth of CdTe. The resulting NPs were 2 nm in diameter with band edge emission at 520 nm and quantum efficiency 15%. The final concentration of CdTe NP was ca. 0.135 mmol, assuming the agglomeration number equal to 65 for 2 nm crystallites.
  • 36
    • 0347796091 scopus 로고    scopus 로고
    • note
    • 3 was used because the excess of L-cysteine present in the mixture as a mild reducing agent converted the initially forming Schiff base into the secondary amine bond.
  • 38
    • 0347166070 scopus 로고    scopus 로고
    • note
    • The gel plates used both for native and for SDS electrophoresis were precast 4-20% linear gradient Tris-HCl gels (Bio-Rad); running times 1.5 h at 82 V, 25 mA. A 10 μL aliquot of the sample was mixed with 2 μL of the sample buffer, and the appropriate amount (2-10 μL) of the mixture was injected into the gel wells to obtain well-resolved spots of desirable dimensions. Sample buffer for SDS-PAGE contained 50% glycerol, 25% 2-mercaptoethanol, 10% SDS, 0.1 bromophenol blue, and Tris-HCl pH 6.8. SDS electrophoresis running buffer was made from 6.06 g of Tris base, 28.5 g of glycine, and 1.0 g of SDS dissolved in 1 L of water.
  • 39
    • 0347796092 scopus 로고    scopus 로고
    • note
    • The sample buffer for native state gel electrophoresis was prepared from 15.5 mL of 1 M Tris-HCl pH 6.8, 2.5 mL of a 1% solution of bromophenol blue, 7 mL of water, and 25 mL of glycerol. The electrophoresis running buffer was made by dissolving 3.0 g of Tris base and 14.4 g of glycine in water and adjusting the volume to 1 L at pH 8.3.
  • 42
    • 0347166071 scopus 로고    scopus 로고
    • note
    • A 0.5 x 0.5 cm piece was cut out from the gel plate after separation of the products of the conjugation reaction. The spot had noticeably yellow coloration indicative of CdTe NPs. The piece of gel was attached to a glass slide and placed in the sample compartment of a Fluorolog 3 instrument. The luminescence spectrum was obtained at the excitation wavelength of 450 nm.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.