Porous alginate/poly(ε-caprolactone) scaffolds: Preparation, characterization and in vitro biological activity

International Journal of Molecular Medicine

Volumn 27, Issue 3, 2011, Pages 455-467

  Grandi, Claudio ^a   Di Liddo, Rosa ^a   Paganin, Piergiorgio ^a   Lora, Silvano ^a   Dalzoppo, Daniele ^a   Feltrin, Giampietro ^a   Giraudo, Chiara ^a   Tommasini, Mara ^a   Conconi, Maria Teresa ^a   Parnigotto, Pier Paolo ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

Bone regeneration; Calcium alginate threads; Poly( caprolactone); Scaffold pore size; Scaffold porosity

Indexed keywords

ALGINIC ACID; ALKALINE PHOSPHATASE; BIOMATERIAL; CALCIUM ALGINATE; FRUCTOSE; MACROGOL; POLYCAPROLACTONE; SUCROSE;

ANIMAL CELL; ARTICLE; BIOCOMPATIBILITY; BONE TISSUE; CELL ADHESION; CELL DIFFERENTIATION; CELL FUNCTION; CELL GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; DIFFUSION; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; IN VITRO STUDY; MOUSE; NONHUMAN; POROSITY; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SEPARATION TECHNIQUE; TISSUE ENGINEERING;

ALGINATES; ANIMALS; BONE AND BONES; CALCIFICATION, PHYSIOLOGIC; CELL LINE, TUMOR; GLUCURONIC ACID; HEXURONIC ACIDS; MICE; POLYESTERS; POROSITY; TISSUE SCAFFOLDS; X-RAY MICROTOMOGRAPHY;

EID: 79551590207     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2010.593     Document Type: Article

References (76)

1. Ciapetti G, Ambrosio L, Savarino L, Granchi D, Cenni E, Baldini N, Pagani S, Guizzardi S, Causa F and Giunti A: Osteoblast growth and function in porous poly epsilon-caprolactone matrices for bone repair: a preliminary study. Biomaterials 24: 3815-3824, 2003.
2. Lin AS, Barrows TH, Cartmell SH and Guldberg RE: Microarchitectural and mechanical characterization of oriented porous polymer scaffolds. Biomaterials 24: 481-489, 2003.
3. Kneser U, Schaefer DJ, Polykandriotis E and Horch RE: Tissue engineering of bone: the reconstructive surgeon's point of view. J Cell Mol Med 10: 7-19, 2006.
4. Banwart JC, Asher MA and Hassanein RS: Iliac crest bone graft harvest donor site morbidity: a statistical evaluation. Spine 20: 1055-1060, 1995.
5. Goulet JA, Senunas LE, DeSilva GL and Greenfield ML: Autogenous iliac crest bone graft: complications and functional assessment. Clin Orthop Relat Res 339: 76-81, 1997.
6. Parikh SN: Bone graft substitutes: past, present, future. J Postgrad Med 48: 142-148, 2002.
7. Datta N, Pham QP, Sharma U, Sikavitsas VI, Jansen JA and Mikos AG: In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation. Proc Natl Acad Sci USA 103: 2488-2493, 2006.
8. Lanza RP and Chick WL: Transplantation of encapsulated cells and tissue. Surgery 121: 1-9, 1997.
9. Patrick CW, Mikos AG and McIntire LV: Prospectus of tissue engineering. In: Frontiers in Tissue Engineering. Patrick CW, Mikos AG and Mclntire LV (eds). Elsevier Ltd., Oxford, pp3-11, 1998.
10. Burg KJL, Porter S and Kellam J: Biomaterial developments for bone tissue engineering. Biomaterials 21: 2347-2359, 2000.
11. Hutmacher DW: Scaffolds in tissue engineering bone and cartilage. Biomaterials 21: 2529-2543, 2000.
12. Salgado AJ, Coutinho OP and Reis RL: Bone tissue engineering: state of the art and future trends. Macromol Biosci 4: 743-765, 2004.
13. Stylios G, Wan T and Giannoudis P: Present status and future potential of enhancing bone healing using nanotechnology. Injury 38 (Suppl1): S63-S74, 2007.
14. Spector M: Biomaterials-based tissue engineering and regenerative medicine solutions to musculoskeletal problems. Swiss Med Wkly 136: 293-301, 2006.
15. Kikuchi M, Suetsugu Y and Tanaka J: Preparation and mechanical properties of calcium phosphate/copoly-L-lactide composites. J Mater Sci Mater Med 8: 361-364, 1997.
16. Marra KG, Campbell PG, DiMilla PA, Kumta PN, Mooney MP, Szem JW and Weiss LE: Novel three-dimensional bio-degradable scaffolds for bone tissue engineering. In: Biomedical Materials: Drug Delivery, Implants, and Tissue Engineering. Proceedings of the Materials Research Society Symposium. Nov 5-9, 1999, Vol. 550. Neenan T, Marcolongo M and Valentini RF (eds.). Material Research Society, Boston, pp155-160, 1999.
17. Böstman OM: Absorbable implants for the fixation of fractures. J Bone Surg 73: 148-153, 1991.
18. Bergsma EJ, Bruijn W, Rozema FR, Bos RM and Boering G: Late tissue response to poly(L-lactide) bone plates and screws. Biomaterials 16: 25-31, 1995.
19. Agrawal CM and Athanasiou KA: Techniques to control pH in the vicinity of biodegrading PLA-PGA implants. J Biomed Mater Res 38: 105-114, 1997.
20. Pitt CG: Poly-ε-caprolactone and its copolymers. In: Biodegradable Polymers as Drug Delivery Systems. Chasin M and Langer R (eds.). Marcel Dekker, New York, pp71-119, 1990.
21. Bezwada RS, Jamiolkowski DD, Lee I, Vishvaroop A, Persivale J, Treka-Benthin S, Erneta M, Suryadevara J, Yang A and Liu S: Monocryl suture, a new ultra-pliable absorbable monofilament suture. Biomaterials 16: 1141-1148, 1995.
22. Benoit MA, Baras B and Gillard J: Preaparation and character- ization of protein-loaded poly (ε-caprolactone) microparticles for oral vccine delivery. Int J Pharm 184: 105-115, 1999.
23. Rohner D, Hutmacher DW, Cheng TK, Oberholzer M and Hammer B: In vivo efficacy of bone-marrow-coated polycaprolactone scaffolds for the reconstruction of orbital defects in the pig. J Biomed Mater Res B Appl Biomater 66: 574-580, 2003.
24. Oh SE, Park IK, Kim JM and Lee JH: In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method. Biomaterials 28: 1664-1671, 2007.
25. Ma G, Song C, Sun H, Yang J and Leng X: A biodegradable levonogestrel-releasing implant made of PCL/F68 compound as tested in rats and dogs. Contraception 74: 141-147, 2006.
26. Sung HJ, Carson M, Johnson C and Galis ZS: The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. Biomaterials 25: 5735-5742, 2004.
27. Petrie Aronin CE, Cooper JA, Sefcik LS, Tholpady SS, Ogle RC and Botchwey EA: Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(epsilon-caprolactone) fabricated via coextrusion and gas foaming. Acta Biomater 4: 1187-1197, 2008.
28. Coombes AGA, Rizzi SC, Williamson M, Barralet JE, Downes S and Wallace WA: Precipitation casting of polycaporlactone for applications in tissue engineering and drug delivery. Biomaterials 25: 315-325, 2003.
29. Ciardelli G, Chiono V, Vozzi G, Pracella M, Ahluwalia A, Barbani N, Cristallini C and Giusti P: Blends of poly (ε-caprolactone) and polysaccharides in tissue engineering applications. Biomacromolecules 6: 1961-1976, 2005.
30. Kweon HY, Yoo MK, Park IK, Kim TH, Lee HC, Lee HS, Oh JS, Akaike T and Cho CS: A novel degradable polycaprolactone networks for tissue engineering. Biomaterials 24: 801-808, 2003.
31. Causa F, Netti PA, Ambrosio L, Ciapetti G, Baldini N, Pagani S, Martini D and Giunti A: Poly-ε-caprolactone/hydroxyapatite composites for bone regeneration: in vitro characterization and human osteoblast response. J Biomed Mater Res A 76: 151-162, 2005.
32. Lam CX, Hutmacher DW, Schantz JT, Woodruff MA and Teoh SH: Evaluation of polycaprolactone scaffold degradation for 6 month in vitro and in vivo. J Biomed Mater Res 90: 906-919, 2009.
33. Zein I, Hutmacher DW, Tan KC and Teoh SH: Fused deposition modeling of novel scaffolds architectures for tissue engineering applications. Biomaterials 23: 1169-1185, 2002.
34. Kuboki Y, Takita H, Kobayashi D, Tsuruga E, Inoue M, Murata M, Nagai N, Dohi Y and Ohgushi H: BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: topology of osteogenesis. J Biomed Mater Res 39: 190-199, 1998.
35. Groeneveld EH, van den Bergh JP, Holzmann P, ten Bruggenkate CM, Tuinzing DB and Burger EH: Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations. J Biomed Mater Res 48: 393-402, 1999.
36. Karageorgiou V and Kaplan D: Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26: 5474-5491, 2005.
37. Story BJ, Wagner WR, Gaisser DM, Cook SD and Rust-Dawicki AM: In vivo performance of a modified CSTi dental implant coating. Int J Oral Maxillofac Implants 13: 749-757, 1998.
38. Hulbert SF, Young FA, Mathews RS, Klawitter JJ, Talbert CD and Steeling FH: Potential of ceramic materials as permanently implantable skeletal prostheses. J Biomed Mater Res 4: 433-456, 1970.
39. Freed LE, Vunjak-Novakovic G, Biron RJ, Eagles DB, Lesnoj DC, Barlow SK and Langer R: Biodegradable polymer scaffolds for tissue engineering. Biotechnology 12: 689-693, 1994.
40. Mikos AG, Bao Y, Cima LG, Ingber DE, Vacanti JP and Langer R: Preparation of Poly(glycolicacid) bonded fiber structures for cell attachment and transplantation. J Biomed Mater Res 27: 183-189, 1993.
41. Mikos AG, Sarakinos G, Leite SM, Vacanti JP and Langer R: Laminated three dimensional biodegradable foams for use in tissue engineering. Biomaterials 14: 323-330, 1993.
42. Mikos AG, Thorsen AJ, Czerwonka LA, Bao Y, Langer R, Winslow DN and Vacanti JP: Preparation and characterization of poly(L-lactic acid) foams. Polymer 35: 1068-1077, 1994.
43. Mooney DJ, Baldwin DF, Suh NP, Vacanti JP and Langer R: Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials 17: 1417-1422, 1996.
44. Nam YS, Yoon JJ and Park TG: A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as a porogen additive. J Biomed Mater Res 53: 1-7, 2000.
45. Lo H, Ponticiello MS and Leong KW: Fabrication of controlled release biodegradable foams by phase separation. Tissue Eng 1: 15-28, 1995.
46. Lo H, Kadiyala S, Guggino SE and Leong KW: Poly(L-lactic acid) foams with cell seeding and controlled-release capacity. J Biomed Mater Res 30: 475-484, 1996.
47. Schugens C, Maquet V, Grandfils C, Jerome R and Teyssie P: Polylactide macroporous biodegradable implants for cell transplantation II. Preparation of polylactide foams for liquid-liquid phase separation. J Biomed Mater Res 30: 449-461, 1996.
48. Nam YS and Park TG: Biodegradable polymeric microcellular foams by modified thermally induced phase separation method. Biomaterials 20: 1783-1790, 1999.
49. Nam YS and Park TG: Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation. J Biomed Mater Res 47: 8-17, 1999.
50. Yoshimoto H, Shin YM, Terai H and Vacanti JP: A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials 24: 2077-2082, 2003.
51. Jang JH, Castani O and Kim HW: Electrospun materials as potential platforms for bone tissue engineering. Adv Drug Deliv Rev 61: 1065-1083, 2009.
52. Mikos AG and Temenoff JA: Formation of highly porous biodegradable scaffolds for tissue engineering. EJB Electronic J Biotech 3: 1-6, 2000.
53. Shang M, Matsuyama H, Maki T, Teremoto M and Lloyd DR: Effect of crystallization and liquid-liquid phase separation on phase separation kinetics in poly(ethylene-co-vinyl alcohol)/glycerol solution. J Polym Sci B Polym Phys 41: 194-201, 2002.
54. Hua FJ, Kim GE, Lee JD, Son YK and Lee DS: Macroporous poly(L-lactide) scaffold 1. Preparation of macroporous scaffold by liquid-liquid phase separation of PLLA-dioxane-water system. J. Biomed Mater Res 63: 161-167, 2002.
55. Shin KC, Kim BS, Kim JH, Park TG, Nam JD and Lee DS: A facile preparation of highly interconnected macroporous PLGA scaffolds by liquid-liquid phase saparation II. Polymer 46: 3801-3808, 2005.
56. Wei G and Ma PX: Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres. J Biomed Mater Res A 78: 306-315, 2006.
57. Smidsrød O and Skjåk-Bræk G: Alginate as immobilization matrix for cells. Trends Biotechnol 8: 71-78, 1990.
58. Oh SH, Kang SG, Kim ES, Cho SH and Lee JH: Fabrication and characterization of hydrophilic poly(lactic-co-glycolic acid)/poly(vinyl alcohol) blend cell scaffolds by melt-molding particulate-leaching method. Biomaterials 24: 4011-4021, 2003.
59. Lewandrowski KU, Hile DD, Thompson BM, Wise DL, Tomford WW and Trantolo DJ: Quantitative measures of osteoinductivity of a porous poly(propylene fumarate) bone graft extender. Tissue Eng 9: 85-93, 2003.
60. Kim HW, Knowles JC and Kim HE: Hydroxyapatite/poly(epsilon caprolactone) composite coatings on hydroxy-apatite porous bone scaffold for drug delivery. Biomaterials 25: 1279-1287, 2004.
61. Leon y Leon CA: New perspectives in mercury porosimetry. Adv Colloid Interface Sci 76-77: 341-372, 1998.
62. Cartmell S, Huynh K, Lin A, Nagaraja S and Guldberg R: Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. J Biomed Mater Res A 69: 97-104, 2004.
63. Hildebrand T, Laib A, Müller R, Dequeker J and Rüegsegger P: Direct three-dimensional morphometric analysis of human cancellous bone: Microstructural data from spine, femur, iliac crest, and calcaneus. J Bone Miner Res 14: 1167-1174, 1999.
64. Ma PX: Scaffolds for tissue fabrication. Materials Today 7: 30-40, 2004.
65. Jowsey J: Studies of haversian systems in man and some animals. J Anat 100: 857-864, 1966.
66. Lanza RP, Langer R and Vacanti J: Principles of Tissue Engineering. Academic Press, San Diego, 2000.
67. Lebourg M, Sabater Serra R, Mas Estelles J, Hernandez Sanches F, Gomez Ribelles JL and Suay Anton J: Biodegradable polycaprolactone scaffold with controlled porosity obtainedby modified particle-leaching tecnique. J Mater Sci Mater Med 19: 2047-2053, 2008.
68. Kuboki Y, Jin Q and Takita H: Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. J Bone Joint Surg Am 83-A (Suppl. 1): S105-S115, 2001.
69. Klawitter JJ and Hulbert SF: Application of porous ceramics for attachment of load bearing internal orthopedic applications. J Biomed Mater Res 5: 161-229, 1971.
70. Cerroni L, Filocamo R, Fabbri M, Piconi C, Caropeso S and Condò SG: Growth of osteoblast-like cells on porous hydroxy-apatite ceramics: an in vitro study. Biomol Eng 19: 119-124, 2002.
71. Domb AJ, Kost J and Wiseman DM: Handbook of Biodegradable Polymers. Harwood Academic Publishers, Amsterdam, 1997.
72. Yang S, Leong KF, Du Z and Chua CK: The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng 7: 679-689, 2001.
73. Currey JD: Mechanical properties of vertebrate hard tissues. Proc. Inst Mech Eng H 212: 399-411, 1998.
74. Yu H, Matthew HW, Wooley PH and Yang SY: Effect of porosity and pore size on microstructures and mechanical properties of poly-ε-caprolactone- hydroxyapatite composites. J Biomed Mater Res B Appl Biomater 86: 541-547, 2008.
75. Choi JY, Lee BH, Song KB, Park RW, Kim IS, Sohn KY, Jo JS and Ryoo HM: Expression patterns of bone-related proteins during osteoblastic differentiation in MC3T3-E1 cells. J Cell Biochem 61: 609-618, 1996.
76. Wang H, Li Y, Zuo Y, Li J, Ma S and Cheng L: Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials 28: 3338-3348, 2007.