Dimeric SecA couples the preprotein translocation in an asymmetric manner

PLoS ONE

Volumn 6, Issue 1, 2011, Pages

  Tang, Ying ^a   Pan, Xijiang ^a   Chen, Yong ^a,c   Tai, Phang C ^b   Sui, Sen Fang ^a  

^a TSINGHUA UNIVERSITY   (China)

^b GEORGIA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DIMER; OLIGOMER; PROTEIN PRECURSOR; PROTEIN PROOMPA; PROTEIN SECA; PROTEIN SECB; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CARRIER PROTEIN; OMPA OUTER MEMBRANE PROTEINS; OUTER MEMBRANE PROTEIN; SECA PROTEIN, BACTERIA; SECB PROTEIN, BACTERIA;

ARTICLE; BACTERIAL MEMBRANE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; ESCHERICHIA COLI; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TARGETING; PROTEIN TRANSPORT; CHEMISTRY; ELECTRON MICROSCOPY; METABOLISM; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; SOLUTION AND SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ADENOSINE TRIPHOSPHATASES; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; ESCHERICHIA COLI; MEMBRANE TRANSPORT PROTEINS; MICROSCOPY, ELECTRON; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN TRANSPORT; SOLUTIONS;

EID: 79551533114     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0016498     Document Type: Article

References (26)

1. Rapoport TA (2007) Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes. Nature 450: 663-669.
2. Randall LL, Topping TB, Hardy SJ, Pavlov MY, Freistroffer DV, et al. (1997) Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins. Proc Natl Acad Sci U S A 94: 802-807.
3. Economou A, Wickner W (1994) SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78: 835-843.
4. Schiebel E, Driessen AJ, Hartl FU, Wickner W (1991) Delta mu H+ and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell 64: 927-939.
5. Triplett TL, Sgrignoli AR, Gao FB, Yang YB, Tai PC, et al. (2001) Functional signal peptides bind a soluble N-terminal fragment of SecA and inhibit its ATPase activity. J Biol Chem 276: 19648-19655.
6. Baud C, Karamanou S, Sianidis G, Vrontou E, Politou AS, et al. (2002) Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain. J Biol Chem 277: 13724-13731.
7. Woodbury RL, Hardy SJ, Randall LL (2002) Complex behavior in solution of homodimeric SecA. Protein Sci 11: 875-882.
8. Economou A (2002) Bacterial secretome: the assembly manual and operating instructions (Review). Mol Membr Biol 19: 159-169.
9. Zhou J, Xu Z (2005) The structural view of bacterial translocation-specific chaperone SecB: implications for function. Mol Microbiol 58: 349-357.
10. Randall LL, Hardy SJ (2002) SecB, one small chaperone in the complex milieu of the cell. Cell Mol Life Sci 59: 1617-1623.
11. Hoffschulte HK, Drees B, Muller M (1994) Identification of a soluble SecA/ SecB complex by means of a subfractionated cell-free export system. J Biol Chem 269: 12833-12839.
12. Osborne AR, Clemons Jr. WM, Rapoport TA (2004) A large conformational change of the translocation ATPase SecA. Proc Natl Acad Sci U S A 101: 10937-10942.
13. Gelis I, Bonvin AM, Keramisanou D, Koukaki M, Gouridis G, et al. (2007) Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR. Cell 131: 756-769.
14. Dekker C, de Kruijff B, Gros P (2003) Crystal structure of SecB from Escherichia coli. J Struct Biol 144: 313-319.
15. Wriggers W, Chacon P (2001) Modeling tricks and fitting techniques for multiresolution structures. Structure 9: 779-788.
16. Chen Y, Pan X, Tang Y, Quan S, Tai PC, et al. (2008) Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryoelectron microscopy. J Biol Chem 283: 28783-28787.
17. Papanikolau Y, Papadovasilaki M, Ravelli RB, McCarthy AA, Cusack S, et al. (2007) Structure of dimeric SecA, the Escherichia coli preprotein translocase motor. J Mol Biol 366: 1545-1557.
18. Lecker S, Lill R, Ziegelhoffer T, Georgopoulos C, Bassford Jr. PJ, et al. (1989) Three pure chaperone proteins of Escherichia coli-SecB, trigger factor and GroEL-form soluble complexes with precursor proteins in vitro. Embo J 8: 2703-2709.
19. Randall LL, Crane JM, Lilly AA, Liu G, Mao C, et al. (2005) Asymmetric binding between SecA and SecB two symmetric proteins: implications for function in export. J Mol Biol 348: 479-489.
20. Papanikou E, Karamanou S, Economou A (2007) Bacterial protein secretion through the translocase nanomachine. Nat Rev Microbiol 5: 839-851.
21. Or E, Navon A, Rapoport T (2002) Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane. Embo J 21: 4470-4479.
22. Wang HW, Chen Y, Yang H, Chen X, Duan MX, et al. (2003) Ring-like pore structures of SecA: implication for bacterial protein-conducting channels. Proc Natl Acad Sci U S A 100: 4221-4226.
23. Muller JP, Ozegowski J, Vettermann S, Swaving J, Van Wely KH, et al. (2000) Interaction of Bacillus subtilis CsaA with SecA and precursor proteins. Biochem J 348(Pt 2): 367-373.
24. Nishiyama K, Suzuki T, Tokuda H (1996) Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85: 71-81.
25. Ludtke SJ, Baldwin PR, Chiu W (1999) EMAN: semiautomated software for high-resolution single-particle reconstructions. J Struct Biol 128: 82-97.
26. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, et al. (2004) UCSF Chimera-a visualization system for exploratory research and analysis. J Comput Chem 25: 1605-1612.