Multiplex PCR assay for the detection and quantification of Campylobacter spp., Escherichia coliO157:H7, and Salmonella serotypes in water samples

FEMS Microbiology Letters

Volumn 316, Issue 1, 2011, Pages 7-15

  Park, Si Hong ^a   Hanning, Irene ^a   Jarquin, Robin ^a   Moore, Philip ^b   Donoghue, Dan J ^a   Donoghue, Ann M ^a,b   Ricke, Steven C ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

^b U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

Campylobacter spp.; E. coli O157:H7; Multiplex PCR; Real time PCR; Salmonella spp.; Watershed

Indexed keywords

BACTERIUM CULTURE; BACTERIUM DETECTION; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; ESCHERICHIA COLI O157; INTERMETHOD COMPARISON; LETTER; MELTING POINT; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; SEROTYPE; WATER SAMPLING; WATERSHED;

BACTERIAL LOAD; CAMPYLOBACTER JEJUNI; DNA, BACTERIAL; ESCHERICHIA COLI O157; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM; STAINING AND LABELING; TRANSITION TEMPERATURE; UNITED STATES; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); CAMPYLOBACTER; CAMPYLOBACTER JEJUNI; ESCHERICHIA; ESCHERICHIA COLI; SALMONELLA; SALMONELLA TYPHIMURIUM;

EID: 79551505135     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02188.x     Document Type: Letter

References (32)

1. Bopp DJ, Sauders BD, Waring AL et al. (2003) Detection, isolation, and molecular subtyping of Escherichia coli O157:H7 and Campylobacter jejuni associated with a large waterborne outbreak. J Clin Microbiol 41: 174-180.
2. Botteldoorn N, Coillie EV, Piessens V, Rasschaert G, Debruyne L, Heyndrickx M, Herman L & Messens W (2008) Quantification of Campylobacter spp. in chicken carcass rinse by real-time PCR. J Appl Microbiol 105: 1909-1918.
3. Centers for Disease Control and Prevention (CDC) (2008) Campylobacter general information. Available at (Accessed May 2010).
4. Chan KF, Tran HL, Kanenaka RY & Kathariou S (2001) Survival of clinical and poultry-derived isolates of Campylobacterjejuni at a low temperature (4°C). Appl Environ Microb 67: 4186-4191.
5. Cheng CM, Lin W, Van KT, Phan L, Tran NN & Farmer D (2008) Rapid detection of Salmonella in foods using real-time PCR. J Food Protect 71: 2436-2441.
6. Clark CG, Price L, Ahmed R, Woodward DL, Melito PL, Rodgers FG, Jamieson F, Ciebin B, Li A & Ellis A (2003) Characterization of waterborne outbreak-associated Campylobacter jejuni, Walerton, Ontario. Emerg Infect Dis 9: 1232-1241.
7. Demeke T & Jenkins GR (2010) Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal Bioanal Chem 396: 1977-1990.
8. D'Souza DH, Critzer FJ & Golden DA (2009) Real-time reverse-transcriptase polymerase chain reaction for the rapid detection of Salmonella using invA primers. Foodborne Pathog Dis 6: 1097-1106.
9. Faúndez G, Troncoso M, Navarrete P & Figueroa G (2004) Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni. BMC Microbiol 4: 19.
10. Fey A, Eichler S, Flavier S, Christen R, Höfle MG & Guzmán CA (2004) Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism. Appl Environ Microb 70: 3618-3623.
11. Gracias KS & Mckillip JL (2004) A review of conventional detection and enumeration methods for pathogenic bacteria in food. Can J Microbiol 50: 883-890.
12. Guy RA, Kapoor A, Holicka J, Shepherd D & Horgen PA (2006) A rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses. J Food Protect 69: 1265-1272.
13. Juen A & Traugott M (2006) Amplification facilitators and multiplex PCR: tools to overcome PCR-inhibition in DNA-gut-content analysis of soil-living invertebrates. Soil Biol Biochem 38: 1872-1879.
14. Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microb 62: 1102-1106.
15. Kussi M, Klemets P, Miettinen I, Laaksonen I, Sarkkinen H, Hanninen ML, Rautelin H, Kela E & Nutri JP (2004) An outbreak of gastroenteritis from a non-chlorinated community water supply. J Epidemiol Commun H 58: 273-277.
16. Leskinen SD & Lim DV (2008) Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of Florida recreational water. Appl Environ Microb 74: 4792-4798.
17. Loge FJ, Thompson DE & Call DR (2002) PCR detection of specific pathogens in water: a risk-based analysis. Environ Sci Technol 36: 2754-2759.
18. Matches JR & Liston J (1968) Low temperature growth of Salmonella. J Food Sci 33: 641-645.
19. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM & Tauxe RV (1999) Food-related illness and death in the United States. Emerg Infect Dis 5: 607-625.
20. Metcalf JH, Reyes-Herrera I, Blore P, Moore PA Jr, Donoghue AM, Hanning I, Ricke SC & Donoghue DJ (2009) Development and validation of methods for the recovery of Salmonella and Campylobacter from watershed runoff after poultry litter application. Poultry Science Association (PSA) Annual Meeting Abstract (# P277), pp. 86-87.
21. 2-degrading compounds. Arch Microbiol 172: 63-67.
22. O'Leary J, Corcoran D & Lucey B (2009) Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens. J Clin Microbiol 47: 3449-3453.
23. O'Reilly CE, Bowen AB, Perez NE et al. (2007) A waterborne outbreak of gastroenteritis with multiple etiologies among resort island visitors and residents: Ohio, 2004. Clin Infect Dis 44: 506-512.
24. Pasmans F, Blahak S, Martel A & Pantchev N (2008) Introducing reptiles into a captive collection: the role of the veterinarian. Vet J 175: 53-68.
25. Pedley S & Howard G (1997) The public health implications of microbiological contamination of groundwater. Q J Eng Geol 30: 179-188.
26. Pickering LK, Marano N, Bocchini JA & Angulo FJ (2008) Exposure to nontraditional pets at home and to animals in public settings: risks to children. Pediatrics 122: 876-886.
27. Rabsch W, Tschäpe H & Bäumler AJ (2001) Non-typhoidal salmonellosis: emerging problems. Microbes Infect 3: 237-247.
28. Rangel JM, Sparling PH, Crowe C, Griffin PM & Swerdlow DL (2005) Epidemiology of Escherchia coli O157:H7 outbreaks, United States, 1982-2002. Emerg Infect Dis 11: 603-609.
29. Sharma VK, Dean-Nvstrom EA & Casey TA (1999) Semi-automated fluorogenic PCR assays (Taqman) for rapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli. Mol Cell Probes 13: 291-302.
30. Shelton DR, Karns JS, Higgins JA, Kessel JASV, Perdue ML, Belt KT, Russell-Anelli J & DebRoy C (2006) Impact of microbial diversity on rapid detection of enterohemorrhagic Escherichia coli in surface waters. FEMS Microbiol Lett 261: 95-101.
31. Topp E, Scott A, Laper DR, Lyautey E & Duriez P (2009) Livestock waste treatment systems for reducing environmental exposure to hazardous enteric pathogens: some considerations. Bioresource Technol 100: 5395-5398.
32. Zhao C, Ge B, Villena JD, Sudler R, Yeh E, Zhao S, White DG, Wagner D & Meng J (2001) Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the greater Washington, D.C., area. Appl Environ Microb 67: 5431-5436.