Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv)

BMC Research Notes

Volumn 4, Issue , 2011, Pages

  Menezes, Márcio A ^a   Aires, Karina A ^a   Ozaki, Christiane Y ^a   Ruiz, Renato M ^a   Pereira, Milton C A ^a   Abreu, Patrícia A E ^a   Elias, Waldir P ^a   Ramos, Oscar H P ^b   Piazza, Roxane M F ^a  

^a INSTITUTO BUTANTAN   (Brazil)

^b SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MUS;

EID: 79251620982     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-4-30     Document Type: Article

References (49)

1. O'Brien AD, Newland JW, Miller SF, Holmes RK, Smith HW, Formal SB: Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 1984, 226:694-696. (Pubitemid 15202147)
2. Jerse AE, Yu J, Tall BD, Kaper JB: A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990, 87:7839-7843.
3. Frankel G, Candy DC, Everest P, Dougan G: Characterization of the Cterminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei. Infect Immun 1994, 62:1835-1842. (Pubitemid 24136984)
4. Adu-Bobie J, Trabulsi LR, Carneiro-Sampaio MMS, Dougan G, Frankel G: Identification of immunodominant regions within the C-terminal cell binding domain of intimin α an intimin β from enteropathogenic Escherichia coli. Infect Immun 1998, 66:5643-5649. (Pubitemid 28531817)
5. Lacher DW, Steinsland H, Whittam TS: Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. FEMS Microbiol Lett 2006, 261:80-87. (Pubitemid 43999705)
6. Agin TS, Wolf MK: Identification of a family of intimins common to Escherichia coli causing attaching-effacing lesions in rabbits, humans, and swine. Infect Immun 1997, 65:320-326. (Pubitemid 27010312)
7. China B, Jacquemin E, Devrin AC, Pirson V, Mainil J: Heterogeneity of the eae genes in attaching/effacing Escherichia coli from cattle: comparison with human strains. Res Microbiol 1999, 150:323-332.
8. Reid SD, Betting DJ, Whittam TS: Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR. J Clin Microbiol 1999, 37:2719-2722. (Pubitemid 29337308)
9. Oswald E, Schmidt H, Morabito S, Karch H, Marches O, Caprioli A: Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 2000, 68:64-71. (Pubitemid 30016678)
10. Tarr CL, Whittam TS: Molecular evolution of the intimin gene in O111 clones of pathogenic Escherichia coli. J Bacteriol 2002, 184:479-487. (Pubitemid 34027388)
11. Zhang WL, Köhler B, Oswald E, Beutin L, Karch H, Morabito S, Caprioli A, Suerbaum S, Schmidt H: Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J Clin Microbiol 2002, 40:4486-4492. (Pubitemid 35434935)
12. Ramachandran V, Brett K, Hornitzky MA, Dowton M, Bettelheim KA, Walker MJ, Djordjevic SP: Distribution of intimin subtypes among Escherichia coli isolates from ruminant and human sources. J Clin Microbiol 2003, 41:5022-5032. (Pubitemid 37429912)
13. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K: Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J Clin Microbiol 2004, 42:1099-1108. (Pubitemid 38332832)
14. Garrido P, Blanco M, Moreno-Paz M, Briones C, Dahbi G, Blanco J, Blanco J, Parro V: STEC-EPEC oligonucleotide microarray: a new tool for typing genetic variants of the LEE pathogenicity island of human and animal Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains. Clin Chem 2006, 52:192-201. (Pubitemid 43185479)
15. Blanco JE, Blanco M, Alonso MP, Mora A, Dahbi G, Coira MA, Blanco J: Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)- producing Escherichia coli isolates from human patients: prevalence in Lugo, Spain, from 1992 through 1999. J Clin Microbiol 2004, 42:311-319.
16. Blanco M, Schumacher S, Tasara T, Zweifel C, Blanco JE, Dahbi G, Blanco J, Stephan R: Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-eta2). BMC Microbiol 2005, 5:23.
17. Blanco M, Blanco JE, Dahbi G, Alonso MP, Mora A, Coira MA, Madrid C, Juárez A, Bernárdez MI, González EA, Blanco J: Identification of two new intimin types in atypical enteropathogenic Escherichia coli. Int Microbiol 2006, 9:103-110. (Pubitemid 44047822)
18. Batchelor M, Knutton S, Caprioli A, Huter V, Zanial M, Dougan G, Frankel G: Development of a universal intimin antiserum and PCR primers. J Clin Microbiol 1999, 37:3822-3827.
19. Koga PCM, Menezes CA, Lima FA, Nara JM, Magalhães CA, Cianciarullo AM, Ferreira-Júnior JMC, Trabulsi LR, Mendes-Ledesma MRB, Piazza RMF: Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC diagnosis. Braz J Microbiol 2003, 34:5-7.
20. Menezes MA, Rocha LB, Koga PC, Fernandes I, Nara JM, Magalhães CA, Abe CM, Ayala CO, Burgos YK, Elias WP, Castro AF, Piazza RM: Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin. J Appl Microbiol 2010, 108:878-887.
21. Svennerholm AM, Wikström M, Lindbald M, Holmgren J: Monoclonal antibodies against Escherichia coli heat-stabile toxin (STa) and their use in a diagnostic ST ganglioside GM1-Enzime-linked immunosorbent assay. J Clin Microbiol 1986, 24:585-590. (Pubitemid 16014555)
22. Weisser NE, Hall JC: Applications of single-chain variable fragment antibodies in therapeutics and diagnostics. Biotechnol Adv 2009, 27:502-520.
23. McCafferty J, Griffiths AD, Winter G, Chiswell DJ: Phage antibodies: filamentous phage displaying antibody variable domains. Nature 1990, 348:552-554. (Pubitemid 120015109)
24. Hanes J, Plückthun A: In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci USA 1997, 94:4937-4942.
25. He M, Taussig MJ: Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites. Nucleic Acids Res 1997, 25:5132-5134. (Pubitemid 28022282)
26. Francisco JA, Stathopoulos C, Warren RA, Kilburn DG, Georgiou G: Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Biotechnology (N Y) 1993, 11:491-495. (Pubitemid 23101632)
27. Wang SH, Zhang JB, Zhang ZP, Zhou YF, Yang RF, Chen J, Guo YC, You F, Zhang XE: Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis. Anal Chem 2006, 78:997-1004. (Pubitemid 43271828)
28. Kühne SA, Hawes WS, La Ragione RM, Woodward MJ, Whitelam GC, Gough KC: Isolation of recombinant antibodies against EspA and intimin of Escherichia coli O157:H7. J Clin Microbiol 2004, 42:2966-2976. (Pubitemid 38938044)
29. Bhaskaran S, Jay CM, Berghman LR, Wagner GG, Waghela SD: A singlechain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein. Vet Res Commun 2005, 29:463-476. (Pubitemid 41548470)
30. Chung WY, Sack M, Carter R, Spiegel H, Fischer R, Hirst TR, Williams NA, James RF: Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heatlabile enterotoxin B-subunit. J Immunol Methods 2008, 339:115-123.
31. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; 1989.
32. Ramos CR, Abreu PA, Nascimento AL, Ho PL: A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide. Braz J Med Biol Res 2004, 37:1103-1109. (Pubitemid 39172546)
33. Warburg O, Christian W: Isolierung und kristallisation des gärungsferments enolase. Biochem Z 1942, 310:384-421.
34. Hwang H, Pierce B, Mintseris J, Janin J, Weng Z: Protein-protein docking benchmark version 3.0. Proteins 2008, 73:705-709. (Pubitemid 352788651)
35. Chaudhury S, Sircar A, Sivasubramanian A, Berrondo M, Gray JJ: Incorporating biochemical information and backbone flexibility in RosettaDock for CAPRI rounds 6-12. Proteins 2007, 69:793-800. (Pubitemid 350210140)
36. Trabulsi LR, Keller R, Gomes TAT: Typical and Atypical Enteropathogenic Escherichia coli. Emerg Infect Dis 2002, 8:508-513. (Pubitemid 34533329)
37. Vilhena-Costa AB, Piazza RM, Nara JM, Trabulsi LR, Martinez MB: Slot blot immunoassay as a tool for plasmid-encoded toxin detection in enteroaggregative Escherichia coli culture supernatants. Diagn Microbiol Infect Dis 2006, 55:101-106. (Pubitemid 43775920)
38. Nara JM, Cianciarullo AM, Culler HF, Bueris V, Horton DS, Menezes MA, Franzolin MR, Elias WP, Piazza RM: Differentiation of typical and atypical enteropathogenic Escherichia coli using colony immunoblot for detection of bundle-forming pilus expression. J Appl Microbiol 2010, 109:35-43.
39. Menezes CA, Gonçalves DS, Amianti J, Fernandes I, Taddei CR, Koga PCM, Trabulsi LR, Martinez MB, Piazza RMF: Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates. Braz J Microbiol 2003, 34:11-13. (Pubitemid 40747432)
40. Menezes CA, Imamura SY, Trabulsi LR, Fernandes-Filho A, Martinez MB, Guth BE, Girão DM, Piazza RM: Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli. Mem Inst Oswaldo Cruz 2006, 101:875-880. (Pubitemid 46129488)
41. Mendes-Ledesma MR, Rocha LB, Bueris V, Krause G, Beutin L, Franzolin MR, Trabulsi LR, Elias WP, Piazza RM: Production and characterization of rabbit polyclonal sera against Shiga toxins Stx1 and Stx2 for detection of Shiga toxin-producing Escherichia coli. Microbiol Immunol 2008, 52:484-491.
42. Neves FO, Ho PL, Raw I, Pereira CA, Moreira C, Nascimento AL: Overexpression of a synthetic gene encoding human alpha interferon in Escherichia coli. Protein Expr Purif 2004, 35:353-359. (Pubitemid 38718098)
43. Reis CV, Andrade SA, Ramos OH, Ramos CR, Ho PL, Batista IF, Chudzinski-Tavassi AM: Lopap, a prothrombin activator from Lonomia obliqua belonging to the lipocalin family: recombinant production, biochemical characterization and structure-function insights. Biochem J 2006, 398:295-302. (Pubitemid 44338486)
44. Silva M, Cabrera-Crespo J, Sbrogio-Almeida ME, Miyaji EN, Ho PL, Leite LC, Lopes AP: Optimizing expression of Streptococcus pneumoniae surface protein a, PspA: serocross-reactivity within families of antisera induced against clades 1 and 3. Mol Biotechnol 2007, 37:146-154.
45. Vieira ML, D'Atri LP, Schattner M, Habarta AM, Barbosa AS, de Morais ZM, Vasconcellos SA, Abreu PA, Gómez RM, Nascimento AL: A novel leptospiral protein increases ICAM-1 and E-selectin expression in human umbilical vein endothelial cells. FEMS Microbiol Lett 2007, 276:172-180. (Pubitemid 47621783)
46. Longhi MT, Oliveira TR, Romero EC, Gonçales AP, de Morais ZM, Vasconcellos SA, Nascimento AL: A newly identified protein of Leptospira interrogans mediates binding to laminin. J Med Microbiol 2009, 58:1275-1282.
47. Atzingen MV, Gómez RM, Schattner M, Pretre G, Gonçales AP, de Morais ZM, Vasconcellos SA, Nascimento AL: Lp95, a novel leptospiral protein that binds extracellular matrix components and activates eselectin on endothelial cells. J Infect 2009, 59:264-276.
48. Vieira ML, de Morais ZM, Gonçales AP, Romero EC, Vasconcellos SA, Nascimento AL: Lsa63, a newly identified surface protein of Leptospira interrogans binds laminin and collagen IV. J Infect 2010, 60:52-64.
49. Guo JQ, You SY, Li L, Zhang YZ, Huang JN, Zhang CY: Construction and high-level expression of a single-chain Fv antibody fragment specific for acidic isoferritin in Escherichia coli. J Biotechnol 2003, 102:177-189. (Pubitemid 36412163)