Multiphoton time-domain fluorescence lifetime imaging microscopy: Practical application to protein-protein interactions using global analysis

Journal of the Royal Society Interface

Volumn 6, Issue SUPPL. 1, 2009, Pages

  Barber, P R ^a,c   Ameer Beg, S M ^a,b   Gilbey, J ^a   Carlin, L M ^b   Keppler, M ^b   Ng, T C ^b   Vojnovic, B ^a,c  

^a Gray Cancer Institute   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Fluorescence lifetime; F rster resonance energy transfer; Global fitting; Time correlated single photon counting; Time domain fluorescence lifetime imaging microscopy

Indexed keywords

ACTIVATION ANALYSIS; ENERGY TRANSFER; FLUORESCENCE; PARTICLE BEAMS; PHOTONS; RESONANCE;

ACQUISITION TIME; BATCH PROCESSING; BIOLOGICAL CELLS; EPIDERMAL GROWTH FACTORS; EXPONENTIAL FITTING; FITTING TECHNIQUES; FLUORESCENCE LIFETIME; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; GLOBAL ANALYSIS; GLOBAL FITTING; IMAGE MANIPULATION; INTERACTING MOLECULES; INTERVAL TIME; LEVENBERG-MARQUARDT; MICROSCOPE SYSTEMS; MULTIPHOTON; PHOTON COUNT; PROTEIN-PROTEIN INTERACTIONS; QUANTITATIVE RESULT; RESONANCE ENERGY TRANSFER; SPATIAL RESOLUTION; TIME DOMAIN; TIME-CORRELATED SINGLE-PHOTON COUNTING; TIME-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY; TIME-RESOLVED;

TIME DOMAIN ANALYSIS;

EPIDERMAL GROWTH FACTOR; PROTEIN CDC42;

ACCURACY; AUTOMATION; BATCH PROCESS; CONFERENCE PAPER; EXPERIMENT; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGING; MICROSCOPE; MULTIPHOTON MICROSCOPY; NOISE; PHOTON; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; SIMULATION; TECHNIQUE; TIME;

EID: 65149092563     PISSN: 17425689     EISSN: 17425662     Source Type: Journal    
DOI: 10.1098/rsif.2008.0451.focus     Document Type: Conference Paper

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