High-yield production and characterization of biologically active GST-tagged human topoisomerase IIα protein in insect cells for the development of a high-throughput assay

Protein Expression and Purification

Volumn 76, Issue 2, 2011, Pages 165-172

  Singh, Praveen K ^a   Chan, Pan F ^b   Hibbs, Martin J ^a   Vazquez, Maria Jesus ^c   Segura, Delfina C ^c   Thomas, Daniel A ^a   Theobald, Andrew J ^a   Gallagher, Kathleen T ^b   Hassan, Namir J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b GLAXOSMITHKLINE   (United States)

^c GLAXOSMITHKLINE   (Spain)

Author keywords

ATPase; DNA; GST; Topoisomerase; Yeast

Indexed keywords

ADENOSINE DIPHOSPHATE; CIRCULAR DNA; DNA BINDING PROTEIN; DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE II ALPHA; ENZYME INHIBITOR; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; TUMOR ANTIGEN;

ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CHEMISTRY; DRUG ANTAGONISM; GENETICS; HIGH THROUGHPUT SCREENING; HUMAN; IC 50; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; SPECTROFLUOROMETRY;

ADENOSINE DIPHOSPHATE; ANIMALS; ANTIGENS, NEOPLASM; BACULOVIRIDAE; CLONING, MOLECULAR; DNA TOPOISOMERASES, TYPE II; DNA, CIRCULAR; DNA-BINDING PROTEINS; ENZYME INHIBITORS; GLUTATHIONE TRANSFERASE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INHIBITORY CONCENTRATION 50; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; SPODOPTERA;

BACTERIA (MICROORGANISMS); HEXAPODA;

EID: 79151483848     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.001     Document Type: Article

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