Development of an insect-cell-based assay for detection of kinase inhibition using NF-κB-inducing kinase as a paradigm

Biochemical Journal

Volumn 419, Issue 1, 2009, Pages 65-73

  Hassan, Namir J ^a   Gul, Sheraz ^a   Flett, Fiona ^a   Hollingsworth, Edward ^a   Dunne, Angela A ^a   Emmons, Amanda J ^a   Hutchinson, Jonathan P ^a   Hibbs, Martin J ^a   Dyos, Susan ^a   Kitson, Jeremy D ^a   Hiley, Emma ^a   Rudiger, Martin ^a   Tew, David G ^a   Powell, David J ^a   Morse, Mary A ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Baculovirus expression system; Cell based assay; Inhibitor of nuclear factor B kinase 1 (IKK1); Nuclear factor B (NF B); Nuclear factor B inducing kinase (NIK)

Indexed keywords

BACULOVIRUS EXPRESSION SYSTEM; BACULOVIRUS EXPRESSION SYSTEMS; BACULOVIRUS SYSTEM; CELL-BASED; CELL-BASED ASSAY; CELL-BASED ASSAYS; EASE OF USE; HIGH-THROUGHPUT SCREENING; INSECT CELLS; KINASE INHIBITION; NUCLEAR FACTORS; PROTEIN EXPRESSION LEVELS; RECOMBINANT PROTEIN; SCALE-UP; SMALL-MOLECULE INHIBITORS; WHOLE CELL;

CELLS; PROTEINS;

ASSAYS;

I KAPPA B KINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LYMPHOTOXIN; PROTEIN KINASE; PROTEIN P52; NF KAPPA B KINASE; NF-KAPPA B KINASE; PROTEIN SERINE THREONINE KINASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL ASSAY; CELL NUCLEUS; CONTROLLED STUDY; DOWNSTREAM PROCESSING; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INDUCTION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SCALE UP; SIGNAL TRANSDUCTION; VIRUS INFECTION; ANIMAL; ARMYWORM; BIOASSAY; BIOLOGICAL MODEL; CELL LINE; HUMAN; METABOLISM; METHODOLOGY; PHOSPHORYLATION; WESTERN BLOTTING;

HEXAPODA;

ANIMALS; BIOLOGICAL ASSAY; BLOTTING, WESTERN; CELL LINE; HUMANS; I-KAPPA B KINASE; MODELS, BIOLOGICAL; NF-KAPPA B; NF-KAPPA B P52 SUBUNIT; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SPODOPTERA;

EID: 62749154597     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20081646     Document Type: Article

References (26)

1. Dancey, J. and Sausville, E. A. (2003) Issues and progress with protein kinase inhibitors for cancer treatment. Nat. Rev. Drug. Discov. 2, 296-313
2. Druker, B. J. (2002) Inhibition of the Bcr-Abl tyrosine kinase as a therapeutic strategy for CML. Oncogene 21, 8541-8546
3. Rubin, B. P., Schuetze, S. M., Eary, J. F., Norwood, T. H., Mirza, S., Conrad, E. U. and Bruckner, J. D. (2002) Molecular targeting of platelet-derived growth factor B by imatinib mesylate in a patient with metastatic dermatofibrosarcoma protuberans. J. Clin. Oncol. 20, 3586-3591
4. Cohen, P. (2002) Protein kinases: the major drugs targets of the twenty-first century? Nat. Rev. Drug Discov. 1, 309-315
5. Barnett, S. P., Defeo-Jones, D., Fu, S., Hancock, P. J., Haskell, K. M., Jones, R. E., Kahana, J. A., Kral, A. M., Leander, K., Lee, L. L. et al. (2005) Identification and characterization of pleckstrin-homology-domain-dependent and isoenzyme-specific Akt inhibitors. Biochem. J. 385, 399-408
6. Fox, S., Farr-Jones, S., Sopchak, L., Boggs, A., Nicely, H. W., Khoury, R. and Biros, M. (2006) High-throughput screening: update on practices and success. J. Biomol. Screen. 11, 864-869
7. Giepmans, B. N., Adams, S. R., Ellisman, M. H. and Tsien, R. Y. (2006) The fluorescent toolbox for assessing protein location and function. Science 312, 217-224
8. Bowen, W. P. and Wylie, P. G. (2006) Application of laser-scanning fluorescence microplate cytometry in high content screening. Assay Drug Dev. Technol. 4, 209-221
9. Condreay, J. P., Ames, R. S., Hassan, N. J., Kost, T. A., Merrihew, R. V., Mossakowska, D. E., Pountney, D. J. and Romanos, M. A. (2006) Baculoviruses and mammalian cell-based assays for drug screening. Adv. Virus Res. 68, 255-286
10. Kost, T. A., Condreay, J. P. and Jarvis, D. L. (2005) Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat. Biotechnol. 23, 567-575
11. Berger, I., Fitzgerald, D. J. and Richmond, T. J. (2004) Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22, 1583-1587
12. Knight, P. J. K., Pfeifer, T. A. and Grigliatti, T. A. (2003) A functional assay for G-protein-coupled receptors using stably transformed insect tissue culture cell lines. Anal. Biochem. 320, 88-103
13. Zhang, J. H., Chung, T. D. and Oldenburg, K. R. (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4, 67-73
14. Frazee, J. S., Hammond, M., Kano, K., Manns, S., Nakamura, H, Thompson, S. K. and Washburn, D. G. (2006) 1H-Pyrrolo[2,3-b]pyridines. Int. Pat. WO 2006/063167
15. Ling, L., Cao, Z. and Goeddel, D. V. (1998) NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. U.S.A. 95, 3792-3797
16. Régnier, C. H., Song, H. Y., Gao, X., Goeddel, D. V., Cao, Z. and Rothe, M. (1997) Identification and characterization of an IκB kinase. Cell 90, 373-383
17. Liao, G., Cao, Z. and Goeddel, D. V. (2004) Regulation of the NF-κB-inducing kinase by tumor necrosis factor receptor-associated factor 3-induced degradation. J. Biol. Chem. 279, 26243-26250
18. Eccleston, J. F., Hutchinson, J. P. and Jameson, D. M. (2005) Fluorescence-based assays. Prog. Med. Chem. 43, 19-48
19. Dejardin, E., Droin, N. M., Delhase, M., Haas, E., Cao, Y., Makris, C., Li, Z. W., Karin, M., Ware, C. F. and Green, D. R. (2002) The lymphotoxin-β receptor induces different patterns of gene expression via two NF-κB pathways. Immunity 17, 525-535
20. Malinin, N. L., Boldin, M. P., Kovalenko, A. V. and Wallach, D. (1997) MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 385, 540-544
21. Xiao, G., Harhaj, E. W. and Sun, S. C. (2001) NF-κB-inducing kinase regulates the processing of NF-κB2 p100. Mol. Cell. 7, 401-409
22. Claudio, E., Brown, K., Park, S., Wang, H. and Siebenlist, U. (2002) BAFF-induced NEMO-independent processing of NF-κB2 in maturing B cells. Nat. Immunol. 3, 958-965
23. Coope, H. J., Atkinson, P. G., Huhse, B., Belich, M., Janzen, J., Holman, M. J., Klaus, G. G., Johnston, L. H. and Ley, S. C. (2002) CD40 regulates the processing of NF-κB2 p100 to p52. EMBO J. 21, 5375-5385
24. Xiao, G., Fong, A. and Sun, S. C. (2004) Induction of p100 processing by NF-κB-inducing kinase involves docking IκB kinase a (IKKα) to p100 and IKKα-mediated phosphorylation. J. Biol. Chem. 279, 30099-30105
25. Lin, X., Mu, Y., Cunningham, Jr, E. T., Marcu, K. B., Geleziunas, R. and Greene, W. C. (1998) Molecular determinants of NF-κB-inducing kinase action. Mol. Cell. Biol. 18, 5899-5907
26. Noguchi, T., Takeda, K., Matsuzawa, A., Saegusa, K., Nakano, H., Gohda, J., Inoue, J. and Ichijo, H. (2005) Recruitment of tumor necrosis factor receptor-associated factor family proteins to apoptosis signal-regulating kinase 1 signalosome is essential for oxidative stress-induced cell death. J. Biol. Chem. 280, 37033-37040