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Volumn 94, Issue , 1999, Pages 223-234

Purification of Baculovirus-Expressed Human DNA Topoisomerase I

Author keywords

Potassium Phosphate; Potassium Phosphate Buffer; Recombinant Baculoviruses; Spinner Flask; Virus Stock

Indexed keywords

DNA TOPOISOMERASE; RECOMBINANT PROTEIN;

EID: 4444325656     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-259-7:223     Document Type: Chapter
Times cited : (10)

References (14)
  • 1
    • 0001498255 scopus 로고
    • Eukaryotic DNA topoisomerases: Two forms of type I DNA topoisomerases from HeLa cell nuclei
    • Liu, L. F. and Miller, K. G. (1981) Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei. Proc. Natl. Acad. Sci. USA 78, 3487–3491.
    • (1981) Proc. Natl. Acad. Sci. USA , vol.78 , pp. 3487-3491
    • Liu, L.F.1    Miller, K.G.2
  • 2
    • 0029986467 scopus 로고    scopus 로고
    • Biochemical and biophysical analyses of recombinant forms of human topoisomerase I
    • Stewart, L., Ireton, G. C., Parker, L. H., Madden, K. R., and Champoux, J. J. (1996) Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271, 7593–7601.
    • (1996) J. Biol. Chem. , vol.271 , pp. 7593-7601
    • Stewart, L.1    Ireton, G.C.2    Parker, L.H.3    Madden, K.R.4    Champoux, J.J.5
  • 3
    • 0026503077 scopus 로고
    • Overexpression of human topoisomerase I in baby hamster kidney cells: Hypersensitivity of clonal isolates to camptothecin
    • Madden, K. R. and Champoux, J. J. (1992) Overexpression of human topoisomerase I in baby hamster kidney cells: hypersensitivity of clonal isolates to camptothecin. Cancer Res. 52, 525–532.
    • (1992) Cancer Res , vol.52 , pp. 525-532
    • Madden, K.R.1    Champoux, J.J.2
  • 4
    • 0024305936 scopus 로고
    • Expression of human DNA topoisomerase I in yeast cells lacking yeast DNA topoisomerase I: Restoration of sensitivity of the cells to the antitumor drug camptothecin
    • Bjornsti, M. A., Benedetti, P., Viglianti, G. A., and Wang, J. C. (1989) Expression of human DNA topoisomerase I in yeast cells lacking yeast DNA topoisomerase I: restoration of sensitivity of the cells to the antitumor drug camptothecin. Cancer Res. 49, 6318–6323.
    • (1989) Cancer Res , vol.49 , pp. 6318-6323
    • Bjornsti, M.A.1    Benedetti, P.2    Viglianti, G.A.3    Wang, J.C.4
  • 6
    • 0001842917 scopus 로고
    • Expression of human type I and II DNA topoisomerases and their derivatives in Escerichia coli
    • Andoh, T., Ikeda, H., and Oguro, M., eds.), CRC, Boca Raton, FL, pp
    • Kikuchi, A. and Miyaike, M. (1993) Expression of human type I and II DNA topoisomerases and their derivatives in Escerichia coli, in Molecular Biology of DNA Topoisomerases and Its Application to Chemotherapy (Andoh, T., Ikeda, H., and Oguro, M., eds.), CRC, Boca Raton, FL, pp. 121–130.
    • (1993) Molecular Biology of DNA Topoisomerases and Its Application to Chemotherapy , pp. 121-130
    • Kikuchi, A.1    Miyaike, M.2
  • 7
    • 0027378859 scopus 로고
    • Baculovirus systems for the expression of human gene products
    • Luckow, V. A. (1993) Baculovirus systems for the expression of human gene products. Curr. Opin. Biotechnol. 4, 564–572.
    • (1993) Curr. Opin. Biotechnol. , vol.4 , pp. 564-572
    • Luckow, V.A.1
  • 8
    • 0029869828 scopus 로고    scopus 로고
    • The domain organization of human topoisomerase I
    • Stewart, L., Ireton, G. C., and Champoux, J. J. (1996) The domain organization of human topoisomerase I. J. Biol. Chem. 271, 7602–7608.
    • (1996) J. Biol. Chem. , vol.271 , pp. 7602-7608
    • Stewart, L.1    Ireton, G.C.2    Champoux, J.J.3
  • 9
    • 85129158076 scopus 로고    scopus 로고
    • Reconstitution of human DNA topoisomerase I activity by fragment complementation
    • Submitted
    • Stewart, L., Ireton, G. C., and Champoux, J. J. (1996) Reconstitution of human DNA topoisomerase I activity by fragment complementation. EMBO J. (Submitted.)
    • (1996) EMBO J
    • Stewart, L.1    Ireton, G.C.2    Champoux, J.J.3
  • 10
    • 0028485758 scopus 로고
    • Overexpression of human DNA topoisomerase I in insect cells using a baculovirus vector
    • Zhelkovsky, A. M. and Moore, C. L. (1994) Overexpression of human DNA topoisomerase I in insect cells using a baculovirus vector. Protein Expr. Purif. 5, 364–370.
    • (1994) Protein Expr. Purif. , vol.5 , pp. 364-370
    • Zhelkovsky, A.M.1    Moore, C.L.2
  • 11
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 14
    • 85129217173 scopus 로고    scopus 로고
    • Alsner, J., Svejstrup, J. Q., Kjeldsen, E., Sorensen, B. S., and Westergaard, O. (1992) Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function. J. Biol. Chem. 267, 12,408–12,411.
    • Alsner, J., Svejstrup, J. Q., Kjeldsen, E., Sorensen, B. S., and Westergaard, O. (1992) Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function. J. Biol. Chem. 267, 12,408–12,411.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.