Human adrenal cells that express both 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production

Journal of Steroid Biochemistry and Molecular Biology

Volumn 123, Issue 3-5, 2011, Pages 122-126

  Nakamura, Yasuhiro ^a   Xing, Yewei ^b   Hui, Xiao Gang ^b   Kurotaki, Yumi ^a   Ono, Katsuhiko ^a   Cohen, Tony ^b   Sasano, Hironobu ^a   Rainey, William E ^b  

^a TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Adrenal gland; Androstenedione; CYB5A; HSD3B2

Indexed keywords

3BETA HYDROXYSTEROID DEHYDROGENASE 2; ANDROSTENEDIONE; CYTOCHROME B5; HYDROXYSTEROID DEHYDROGENASE; UNCLASSIFIED DRUG;

ADRENAL CORTEX; ADRENAL GLAND; ARTICLE; HUMAN; HUMAN CELL; HUMAN TISSUE; NORMAL HUMAN; PROTEIN EXPRESSION; ZONA FASCICULATA; ZONA GLOMERULOSA;

ADRENAL GLANDS; ANDROSTENEDIONE; CYTOCHROMES B5; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; PROGESTERONE REDUCTASE; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 79151469458     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2010.12.001     Document Type: Article

References (34)

1. W.D. Odell, and L.N. Parker Control of adrenal androgen production Endocr. Res. 10 1984-1985 617 630
2. C. Labrie, J. Simard, H.F. Zhao, A. Bélanger, G. Pelletier, and G.F. Labrie Stimulation of androgen-dependent gene expression by the adrenal precursors dehydroepiandrosterone and androstenedione in the rat ventral prostate Endocrinology 124 1989 2745 2754
3. W. de Ronde, A. Hofman, H.A. Pols, and F.H. de Jong A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women Eur. J. Endocrinol. 152 2005 261 268
4. F. Bidlingmaier, H.G. Dörr, W. Eisenmenger, U. Kuhnle, and D. Knorr Contribution of the adrenal gland to the production of androstenedione and testosterone during the first two years of life J. Clin. Endocrinol. Metab. 62 1986 331 335
5. C.E. Flück, W.L. Miller, and R.J. Auchus The 17,20-lyase activity of cytochrome p450c17 from human fetal testis favors the delta5 steroidogenic pathway J. Clin. Endocrinol. Metab. 88 2003 3762 3766
6. M.K. Akhtar, S.L. Kelly, and M.A. Kaderbhai Cytochrome b(5) modulation of 17{alpha} hydroxylase and 17-20 lyase (CYP17) activities in steroidogenesis J. Endocrinol. 187 2005 267 274
7. W.E. Rainey, and Y. Nakamura Regulation of the adrenal androgen biosynthesis J. Steroid. Biochem. Mol. Biol. 108 2008 281 286
8. J. Simard, Y. de Launoit, and F. Labrie Characterization of the structure-activity relationships of rat types I and II 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase by site-directed mutagenesis and expression in HeLa cells J. Biol. Chem. 266 1991 14842 14845
9. F. Labrie, V. Luu-The, A. Belanger, S.X. Lin, J. Simard, G. Pelletier, and C. Labrie Is dehydroepiandrosterone a hormone? J. Endocrinol. 187 2005 169 196
10. S. Dharia, A. Slane, M. Jian, M. Conner, A.J. Conley, and C.R. Parker Jr. Colocalization of P450c17 and cytochrome b5 in androgen-synthesizing tissues of the human Biol. Reprod. 71 2004 83 88
11. J. Simard, M.L. Ricketts, S. Gingras, P. Soucy, F.A. Feltus, and M.H. Melner Molecular biology of the 3beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase gene family Endocr. Rev. 26 2005 525 582
12. Y. Nakamura, Y. Xing, H. Sasano, and W.E. Rainey The mediator complex subunit 1 enhances transcription of genes needed for adrenal androgen production Endocrinology 150 2009 4145 4153
13. Y. Nakamura, S. Aoki, Y. Xing, H. Sasano, and W.E. Rainey Metastin stimulates aldosterone synthesis in human adrenal cells Reprod. Sci. 14 2007 836 845
14. A.F. Gazdar, H.K. Oie, C.H. Shackleton, T.R. Chen, T.J. Triche, C.E. Myers, G.P. Chrousos, M.F. Brennan, C.A. Stein, and R.V. La Rocca Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis Cancer Res. 50 1990 5488 5496
15. E. Samandari, P. Kempná, J.M. Nuoffer, G. Hofer, P.E. Mullis, and C.E. Flück Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities J. Endocrinol. 195 2007 459 472
16. N. Huang, A. Dardis, and W.L. Miller Regulation of cytochrome b5 gene transcription by Sp3, GATA-6, and steroidogenic factor 1 in human adrenal NCI-H295A cells Mol. Endocrinol. 19 2005 2020 2034
17. E.I. Georgiadis, C. Matzoros, C. Aliferis, and M. Batrinos Are adrenal and testicular androgen levels correlated? Horm. Metab. Res. 24 1992 488 491
18. H.G. Burger Androgen production in women Fertil. Steril. 77 2002 S3 S5
19. T. Piltonen, R. Koivunen, L. Morin-Papunen, A. Ruokonen, I.T. Huhtaniemi, and J.S. Tapanainen Ovarian and adrenal steroid production: regulatory role of LH/HCG Hum. Reprod. 17 2002 620 624
20. B. Couzinet, G. Meduri, M.G. Lecce, J. Young, S. Brailly, H. Loosfelt, E. Milgrom, and G. Schaison The postmenopausal ovary is not a major androgen-producing gland J. Clin. Endocrinol. Metab. 86 2001 5060 5066
21. C. Sawetawan, L. Milewich, R.A. Word, B.R. Carr, and W.E. Rainey Compartmentalization of type I 17 beta-hydroxysteroid oxidoreductase in the human ovary Mol. Cell. Endocrinol. 99 1994 161 168
22. T. Suzuki, H. Sasano, M. Tamura, H. Aoki, T. Fukaya, A. Yajima, H. Nagura, and J.I. Mason Temporal and spatial localization of steroidogenic enzymes in premenopausal human ovaries: in situ hybridization and immunohistochemical study Mol. Cell. Endocrinol. 97 1993 135 143
23. T. Suzuki, H. Sasano, J. Takeyama, C. Kaneko, W.A. Freije, B.R. Carr, and W.E. Rainey Developmental changes in steroidogenic enzymes in human postnatal adrenal cortex: immunohistochemical studies Clin. Endocrinol. (Oxf.) 53 2000 739 747
24. J.S. Gell, B.R. Carr, H. Sasano, B. Atkins, L. Margraf, J.I. Mason, and W.E. Rainey Adrenarche results from development of a 3beta-hydroxysteroid dehydrogenase-deficient adrenal reticularis J. Clin. Endocrinol. Metab. 83 1998 3695 3701
25. A. Dardis, N. Saraco, M.A. Rivarola, and A. Belgorosky Decrease in the expression of the 3beta-hydroxysteroid dehydrogenase gene in human adrenal tissue during prepuberty and early puberty: implications for the mechanism of adrenarche Pediatr. Res. 45 1999 384 388
26. W. Wang, L. Yang, T. Suwa, P.R. Casson, and P.J. Hornsby Differentially expressed genes in zona reticularis cells of the human adrenal cortex Mol. Cell. Endocrinol. 173 2001 127 134
27. W.E. Rainey, I.M. Bird, C. Sawetawan, N.A. Hanley, J.L. McCarthy, E.A. McGee, R. Wester, and J.I. Mason Regulation of human adrenal carcinoma cell (NCI-H295) production of C19 steroids J. Clin. Endocrinol. Metab. 77 1993 731 737
28. W.E. Rainey, B.R. Carr, H. Sasano, T. Suzuki, and J.I. Mason Dissecting human adrenal androgen production Trends Endocrinol. Metab. 13 2002 234 239
29. M. Katagiri, N. Kagawa, and M.R. Waterman The role of cytochrome b5 in the biosynthesis of androgens by human P450c17 Arch. Biochem. Biophys. 317 1995 343 347
30. 5: endocrinological and mechanistic implications Biochem. J. 308 1995 901 908
31. R.J. Auchus, T.C. Lee, and W.L. Miller Cytochrome b5 augments the 17,20 lyase activity of human P450c17 without direct electron transfer J. Biol. Chem. 273 1998 3158 3165
32. B.J. Brock, and M.R. Waterman Biochemical differences between rat and human cytochrome P450c17 support the different steroidogenic needs of these two species Biochemistry 38 1999 1598 1606
33. Y. Ideyama, M. Kudoh, K. Tanimoto, Y. Susaki, T. Nanya, T. Nakahara, H. Ishikawa, T. Fujikura, H. Akaza, and H. Shikama YM116, 2-(1H-imidazol-4- ylmethyl)-9H-carbazole, decreases adrenal androgen synthesis by inhibiting C17-20 lyase activity in NCI-H295 human adrenocortical carcinoma cells Jpn J. Pharmacol. 79 1999 213 220
34. A.J. Conley, and I.M. Bird The role of cytochrome P450 17 alpha-hydroxylase and 3 beta-hydroxysteroid dehydrogenase in the integration of gonadal and adrenal steroidogenesis via the delta 5 and delta 4 pathways of steroidogenesis in mammals Biol. Reprod. 56 1997 789 799