Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3′ conserved segment structure

Antimicrobial Agents and Chemotherapy

Volumn 55, Issue 2, 2011, Pages 943-945

  Lee, Mei Feng ^a,b   Peng, Chien Fang ^a   Hsu, Hui Jine ^b   Toh, Han Siong ^b  

^a KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

^b CHI MEI MEDICAL CENTER   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

EXTRACHROMOSOMAL DNA;

3' UNTRANSLATED REGION; ARTICLE; CONJUGATION; DNA SEQUENCE; ENTEROBACTER CLOACAE; GENE MUTATION; GENE STRUCTURE; GENETIC ASSOCIATION; INTEGRON; INVERSE POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA; SOUTHERN BLOTTING;

ANIMALS; BACTERIAL PROTEINS; BLOTTING, SOUTHERN; CONJUGATION, GENETIC; CONSERVED SEQUENCE; DRUG RESISTANCE, BACTERIAL; ENTEROBACTER CLOACAE; HUMANS; INTEGRONS; PLASMIDS; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA; SEQUENCE ANALYSIS, DNA; SULFONAMIDES;

EID: 78751689750     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00988-10     Document Type: Article

References (18)

1. Ahmed, A. M., and T. Shimamoto. 2004. A plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan. FEMS Microbiol. Lett. 235:243-248.
2. Antunes, P., J. Machado, and L. Peixe. 2007. Dissemination of sul3-containing elements linked to class 1 integrons with an unusual 3′ conserved sequence region among Salmonella isolates. Antimicrob. Agents Chemother. 51:1545-1548.
3. Bae, J. H., and J. H. Sohn. 2010. Template-blocking PCR: an advanced PCR technique for genome walking. Anal. Biochem. 398:112-116.
4. Bischoff, K. M., D. G. White, M. E. Hume, T. L. Poole, and D. J. Nisbet. 2005. The chloramphenicol resistance gene cmlA is disseminated on transferable plasmids that confer multiple-drug resistance in swine Escherichia coli. FEMS Microbiol. Lett. 243:285-291.
5. Cano, M. E., et al. 2009. Detection of plasmid-mediated quinolone resistance genes in clinical isolates of Enterobacter spp. in Spain. J. Clin. Microbiol. 47:2033-2039.
6. Collins, F. S., and S. M. Weissman. 1984. Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method. Proc. Natl. Acad. Sci. U. S. A. 81:6812-6816.
7. Fluit, A. C., and F. J. Schmitz. 2004. Resistance integrons and super-integrons. Clin. Microbiol. Infect. 10:272-288.
8. Garnier, F., N. Raked, A. Gassama, F. Denis, and M. C. Ploy. 2006. Genetic environment of quinolone resistance gene qnrB2 in a complex sul1-type integron in the newly described Salmonella enterica serovar Keurmassar. Antimicrob. Agents Chemother. 50:3200-3202.
9. Hall, R. M., and C. M. Collis. 1995. Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol. Microbiol. 15:593-600.
10. Kado, C. I., and S. T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145:1365-1373.
11. Kotik, M. 2009. Novel genes retrieved from environmental DNA by polymerase chain reaction: current genome-walking techniques for future metagenome applications. J. Biotechnol. 144:75-82.
12. Lee, M. F., Y. H. Chen, and C. F. Peng. 2009. Molecular characterisation of class 1 integrons in Salmonella enterica serovar Choleraesuis isolates from southern Taiwan. Int. J. Antimicrob. Agents 33:216-222.
13. Lee, M. F., C. F. Peng, H. J. Hsu, and Y. H. Chen. 2008. Molecular characterisation of the metallo-beta-lactamase genes in imipenem-resistant Gramnegative bacteria from a university hospital in southern Taiwan. Int. J. Antimicrob. Agents 32:475-480.
14. Lévesque, C., L. Piche, C. Larose, and P. H. Roy. 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 39:185-191.
15. Nandi, S., J. J. Maurer, C. Hofacre, and A. O. Summers. 2004. Gram-positive bacteria are a major reservoir of class 1 antibiotic resistance integrons in poultry litter. Proc. Natl. Acad. Sci. U. S. A. 101:7118-7122.
16. Ochman, H., A. S. Gerber, and D. L. Hartl. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623.
17. Post, V., G. D. Recchia, and R. M. Hall. 2007. Detection of gene cassettes in Tn402-like class 1 integrons. Antimicrob. Agents Chemother. 51:3467-3468.
18. Wu, J. J., W. C. Ko, S. H. Tsai, and J. J. Yan. 2007. Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital. Antimicrob. Agents Chemother. 51:1223-1227.