Template-blocking PCR: An advanced PCR technique for genome walking

Analytical Biochemistry

Volumn 398, Issue 1, 2010, Pages 112-116

  Bae, Jung Hoon ^a   Sohn, Jung Hoon ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Cassette ligation mediated PCR; Genome walking; Template blocking PCR

Indexed keywords

CLONE CELLS; DNA; GENES;

CONTROL EXPERIMENTS; GENE-SPECIFIC PRIMERS; GENOME-WALKING; GENOMIC FRAGMENTS; LIGATION-MEDIATED PCR; POLYMERASE CHAIN REACTION TECHNIQUES; RESTRICTION ENZYMES; TEMPLATE-BLOCKING PCR;

POLYMERASE CHAIN REACTION;

2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; CELLULASE; GENOMIC DNA; PHOSPHOGLYCERATE KINASE; PROTEIN PGK1; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA FLANKING REGION; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; GENE CASSETTE; GENE ISOLATION; GENE TARGETING; GENOME ANALYSIS; GENOMIC FRAGMENT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PENICILLIUM; PICHIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION;

PENICILLIUM SP.; PICHIA CIFERRII;

EID: 73549111095     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.11.003     Document Type: Article

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