Sialylation enhancement of CTLA4-Ig fusion protein in chinese hamster ovary cells by dexamethasone

Biotechnology and Bioengineering

Volumn 107, Issue 3, 2010, Pages 488-496

  Jing, Ying ^a   Qian, Yueming ^a   Li, Zheng Jian ^a  

^a BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

CHO; CTLA4 Ig fusion protein; Dexamethasone; Glucocorticoids; Glycosylation; Mammalian cell culture

Indexed keywords

CHO; DEXAMETHASONES; FUSION PROTEINS; GLUCOCORTICOIDS; MAMMALIAN CELL CULTURE;

ANIMAL CELL CULTURE; BIOREACTORS; CARBOXYLIC ACIDS; ENZYME ACTIVITY; ESTERIFICATION; GLYCOSYLATION; MAMMALS; PROTEINS; CELLS; CYTOLOGY; HORMONES;

CELL CULTURE; GLYCOSYLATION;

BETA N ACETYLGLUCOSAMINYLGLYCOPEPTIDE BETA 1,4 GALACTOSYLTRANSFERASE; CYTOTOXIC T LYMPHOCYTE ANTIGEN 4; DEXAMETHASONE; GLYCOSYLTRANSFERASE; HYBRID PROTEIN; IMMUNOGLOBULIN; MIFEPRISTONE; SIALIC ACID; SIALYLTRANSFERASE; ALPHA 2,3 SIALYLTRANSFERASE; BETA 1,4 GALACTOSYLTRANSFERASE; GALACTOSYLTRANSFERASE; GLUCOCORTICOID RECEPTOR; HYDROCORTISONE; PREDNISOLONE; SIALIDASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL DIVISION; CELL VIABILITY; CHINESE HAMSTER; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PROTEIN EXPRESSION; SIALYLATION; AMINO TERMINAL SEQUENCE; ANIMAL CELL CULTURE; CULTURE MEDIUM; ENZYME REPRESSION; FED BATCH REACTOR; FEMALE; HORMONE RECEPTOR INTERACTION; PROTEIN CLEAVAGE; PROTEIN CONTENT; PROTEIN SYNTHESIS;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DEXAMETHASONE; GENE EXPRESSION; IMMUNOCONJUGATES; MIFEPRISTONE; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; RECEPTORS, GLUCOCORTICOID; SIALYLTRANSFERASES;

CRICETULUS GRISEUS; MAMMALIA;

EID: 78651509902     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22827     Document Type: Article

References (33)

1. Barnes PJ. 1998. Anti-inflammatory actions of glucocorticoids: Molecular mechanisms. Clin Sci (Lond) 94(6):557-572.
2. Basa LJ, Spellman MW. 1990. Analysis of glycoprotein-derived oligosaccharides by high-pH anion-exchange chromatography. J Chromatogr 499:205-220.
3. Coughlan CM, Breen KC. 1998. Glucocorticoid induction of the alpha2,6 sialyltransferase enzyme in a mouse neural cell line. J Neurosci Res 51(5):619-626.
4. Crowell CK, Grampp GE, Rogers GN, Miller J, Scheinman RI. 2007. Amino acid and manganese supplementation modulates the glycosylation state of erythropoietin in a CHO culture system. Biotechnol Bioeng 96(3):538-549.
5. Debray H, Decout D, Strecker G, Spik G, Montreuil J. 1981. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to Nglycosylproteins. Eur J Biochem 117(1):41-55.
6. Eisenstein A. 1973. Effects of adrenal cortical hormones on carbohydrate, protein, and fat metabolism. Am J Clin Nutr 26(1):113-120.
7. Fukuta K, Yokomatsu T, Abe R, Asanagi M, Makino T. 2000. Genetic engineering of CHO cells producing human interferon-gamma by transfection of sialyltransferases. Glycoconj J 17(12):895-904.
8. Gramer MJ. 2000. Detecting and minimizing glycosidase activities that can hydrolyze sugars from cell culture-produced glycoproteins. Mol Biotechnol 15(1):69-75.
9. Gramer MJ, Goochee CF. 1993. Glycosidase activities in Chinese hamster ovary cell lysate and cell culture supernatant. Biotechnol Prog 9(4):366-373.
10. Gramer MJ, Goochee CF. 1994. Glycosidase activities of the 293 and NS0 cell lines, and of an antibody-producing hybridoma cell line. Biotechnol Bioeng 43(5):423-428.
11. Gramer MJ, Schaffer DV, Sliwkowski MB, Goochee CF. 1994. Purification and characterization of alpha-L-fucosidase from Chinese hamster ovary cell culture supernatant. Glycobiology 4(5):611-616.
12. Gramer MJ, Goochee CF, Chock VY, Brousseau DT, Sliwkowski MB. 1995. Removal of sialic acid from a glycoprotein in CHO cell culture supernatant by action of an extracellular CHO cell sialidase. Biotechnology (NY) 13(7):692-698.
13. Gu X, Harmon BJ, Wang DI. 1997. Site- and branch-specific sialylation of recombinant human interferon-gamma in Chinese hamster ovary cell culture. Biotechnol Bioeng 55(2):390-398.
14. Hamr A, Vlasakova V, Kolinska J. 1993. Alpha 2,6-sialyltransferase predominates in cultured jejunum of suckling rats: It is up-regulated by dexamethasone and secreted during cultivation. Biochim Biophys Acta 1157(3):285-289.
15. Hossler P, Khattak SF, Li ZJ. 2009. Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology 19(9):936-949.
16. Jefferis R. 2005. Glycosylation of recombinant antibody therapeutics. Biotechnol Prog 21(1):11-16.
17. Jefferis R. 2009. Glycosylation as a strategy to improve antibody-based therapeutics. Nat Rev Drug Discov 8(3):226-234.
18. Jenkins N, Curling EMA. 1994. Glycosylation of recombinant proteins: Problems and prospects. Enzyme Microb Technol 16:354-364.
19. Jeong YT, Choi O, Lim HR, Son YD, Kim HJ, Kim JH. 2008. Enhanced sialylation of recombinant erythropoietin in CHO cells by human glycosyltransferase expression. J Microbiol Biotechnol 18(12):1945-1952.
20. Jeong YT, Choi O, Son YD, Park SY, Kim JH. 2009. Enhanced sialylation of recombinant erythropoietin in genetically engineered Chinese-hamster ovary cells. Biotechnol Appl Biochem 52(Pt 4):283-291.
21. Jing Y, Qian Y, Li Z. 2009. Mammalian cell culture processes for protein production. US provisional patent appl. No. 61/278343.
22. Kolinska J, Zakostelecka M, Hamr A, Baudysova M. 1996. Coordinate expression of beta-galactoside alpha 2,6-sialyltransferase mRNA and enzyme activity in suckling rat jejunum cultured in different media: Transcriptional induction by dexamethasone. J Steroid Biochem Mol Biol 58(3):289-297.
23. Lee EU, Roth J, Paulson JC. 1989. Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of beta-galactoside alpha 2,6-sialyltransferase. J Biol Chem 264(23):13848-13855.
24. Ngantung FA, Miller PG, Brushett FR, Tang GL, Wang DI. 2006. RNA interference of sialidase improves glycoprotein sialic acid content consistency. Biotechnol Bioeng 95(1):106-119.
25. Raux-Demay MC, Pierret T, Bouvier d'Yvoire M, Bertagna X, Girard F. 1990. Transient inhibition of RU 486 antiglucocorticoid action by dexamethasone. J Clin Endocrinol Metab 70(1):230-233.
26. Townsend RR, Hardy MR. 1991. Analysis of glycoprotein oligosaccharides using high-pH anion exchange chromatography. Glycobiology 1(2): 139-147.
27. Vandamme V, Pierce A, Verbert A, Delannoy P. 1993. Transcriptional induction of beta-galactoside alpha-2,6-sialyltransferase in rat fibroblast by dexamethasone. Eur J Biochem 211(1-2):135-140.
28. Wang XC, O'Hanlon TP, Lau JT. 1989. Regulation of beta-galactoside alpha 2,6-sialyltransferase gene expression by dexamethasone. J Biol Chem 264(3):1854-1859.
29. Wang XC, Smith TJ, Lau JT. 1990. Transcriptional regulation of the liver beta-galactoside alpha 2,6-sialyltransferase by glucocorticoids. J Biol Chem 265(29):17849-17853.
30. Warner TG, Sliwkowski MB. 1998. Sialidase-deficient cells EP0700443.
31. Weikert S, Papac D, Briggs J, Cowfer D, Tom S, Gawlitzek M, Lofgren J, Mehta S, Chisholm V, Modi N, Eppler S, Carroll K, Chamow S, Peers D, Berman D, Krunmen. 1999. Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins. Nat Biotechnol 17(11): 1116-1121.
32. Weiss P, Ashwell G. 1989. The asialoglycoprotein receptor: Properties and modulation by ligand. Prog Clin Biol Res 300:169-184.
33. Zhang X, Lok SH, Kon OL. 1998. Stable expression of human alpha-2,6-sialyltransferase in Chinese hamster ovary cells: Functional consequences for human erythropoietin expression and bioactivity. Biochim Biophys Acta 1425(3):441-452.