GC-clamp primer batches yield 16S rRNA gene amplicon pools with variable GC clamps, affecting denaturing gradient gel electrophoresis profiles

FEMS Microbiology Letters

Volumn 312, Issue 1, 2010, Pages 55-62

  Rettedal, Elizabeth A ^a   Clay, Sharon ^a   Brözel, Volker S ^a  

^a SOUTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

Bacterial diversity; DGGE; GC clamp; PCR; RRNA

Indexed keywords

CYTOSINE; GENOMIC DNA; GUANOSINE; RIBOSOME RNA; RNA 16S;

AMPLICON; BACTERIAL STRAIN; DENATURING GRADIENT GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE SEQUENCE; LETTER; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SOIL MICROFLORA;

BACTERIA; BASE SEQUENCE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA PRIMERS; MOLECULAR SEQUENCE DATA; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 78651485275     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02097.x     Document Type: Letter

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