Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology

Cytotechnology

Volumn 62, Issue 6, 2010, Pages 557-571

  Knöspel, Fanny ^a   Schindler, Rudolf K ^b   Lübberstedt, Marc ^a   Petzolt, Stephanie ^b   Gerlach, Jörg C ^c   Zeilinger, Katrin ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Biochrom AG   (Germany)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Culture medium optimization; Design of experiments; Expansion; Growth factors; Mouse embryonic stem cells

Indexed keywords

CYSTEINE; INSULIN; LEUKEMIA INHIBITORY FACTOR; ZINC;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CULTURE MEDIUM; DESIGN OF EXPERIMENT; EMBRYO; EMBRYONIC STEM CELL; EXPERIMENTAL DESIGN; FACTORIAL ANALYSIS; HUMAN; IN VITRO STUDY; METHODOLOGY; MOUSE; MOUSE EMBRYO; NONHUMAN; SERUM; SERUM FREE CULTURE MEDIUM;

EID: 78651470151     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-010-9307-8     Document Type: Article

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