Dynamic 3D culture promotes spontaneous embryonic stem cell differentiation in vitro

Tissue Engineering - Part C: Methods

Volumn 16, Issue 1, 2010, Pages 115-121

  Gerlach, Jörg C ^a   Hout, Mariah ^a   Edsbagge, Josefina ^b   Björquist, Petter ^b   Lübberstedt, Marc ^c   Miki, Toshio ^a   Stachelscheid, Harald ^c   Schmelzer, Eva ^a   Schatten, Gerald ^d   Zeilinger, Katrin ^c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b Cellartis AB   (Sweden)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; CELL CULTURE; CELL GROWTH; THREE DIMENSIONAL;

3D CULTURE; BIOREACTOR CULTURES; CONTINUOUS MEDIUM; EMBRYONIC STEM CELLS; IN-VITRO; MOUSE EMBRYONIC STEM CELLS; PETRI DISH; THREE-DIMENSIONAL (3D); TISSUE DEVELOPMENT; TISSUE STRUCTURE;

STEM CELLS;

OXYGEN;

ANIMAL; ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; CHEMISTRY; COCULTURE; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; EMBRYONIC STEM CELL; FLUORESCENCE MICROSCOPY; IN VITRO STUDY; METHODOLOGY; MOUSE; PERFUSION; THREE DIMENSIONAL IMAGING; TIME; TISSUE ENGINEERING; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; COCULTURE TECHNIQUES; CULTURE MEDIA; EMBRYONIC STEM CELLS; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; OXYGEN; PERFUSION; TIME FACTORS; TISSUE ENGINEERING;

EID: 77952382729     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2008.0654     Document Type: Article

References (34)

1. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. Embryonic stem cell lines derived from human embryonic blastocysts. Science 282, 1145, 1998.
2. Wiles, M.V. Embryonic stem cell differentiation in vitro. Methods Enzymol 225, 900, 1993.
3. Cukierman, E., Pankov, R., and Yamada, K.M. Embryonic stem cell differentiation in vitro. Curr Opin Cell Biol 14, 633, 2002.
4. Abbott, A. Cell culture: biology's new dimension. Nature 424, 870, 2003.
5. Knazek, R.A., Gullino, P.M., Kohler, P.O., and Dedrick, R.L. Cell culture on artificial capillaries: an approach to tissue growth in vitro. Science 178, 65, 1972.
6. Gerlach, J.C., Mutig, K., Zeilinger, K., Sauer, I.M., Mieder, T., Naumann, G., Gruenwald, A., Pleß, G., Schrade, P., Efimova, E.,Goetz, M.,Mas,A.,Vienken, J., Bachmann, S., andNeuhaus, P. The use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors\a morphological study. Transplantation 76, 781, 2003.
7. Monga, P.S., Hout, M.S., Baun, M.J., Micsenyi, A., Muller, P., Tummalapalli, L., Ranade, A.R., Strom, S.C., and Gerlach, J.C. Mouse fetal liver cells in artificial capillary beds in three-dimensional four-compartment bioreactors. Am J Pathol 167, 1279, 2005.
8. Xu, A.S.L., Luntz, T.L., Macdonalds, J.M., Kubota, H., Hsu, E., London, R.E., and Reid, L.M. Lineage and biology and liver. In: Lanza, R.P., Langer, R., and Vacanti, J., eds. Principles of Tissue Engineering, Volume 41, 2nd edition. Academic Press, San Diego, CA, 1999, pp. 559-598.
9. Smith, A.G. Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 78, 435, 2001.
10. Williams, R.L., Hilton, D.J., Pease, S., Wilson, T.A., Stewart, C.L., Gearing, D.P., Wagner, E.F., Metcalf, D., Nicola, N.A., and Gough, N.M. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells Nature 336, 684, 1988.
11. Dahlstrand, J., Lardelli, M., and Lendahl, U. Nestin mRNA expression correlates with the central nervous system progenitor cell state in many, but not all, regions of developing central nervous system. Brain Res Dev Brain Res 84, 109, 1995.
12. Ang, S.L., Wierda, A., Wong, D., Stevens, K.A., Cascio, S., Rossant, J., and Zaret, K.S. The formation and maintenance of the definitive endoderm lineage in the mouse: involvement of HNF3=forkhead proteins. Development 119, 1301, 1993.
13. Clement, S., Stouffs, M., Bettiol, E., Kampf, S., Krause, K., Chaponnier, C., and Jaconi, M. Expression and function of a-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation. J Cell Sci 120, 229, 2007.
14. Katsetos, C.D., Herman, M.M., and Mork, S.J. Class III b-tubulin in human development and cancer. Cell Motil Cytoskeleton 55, 77, 2003.
15. Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Schöler, H., and Smith, A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95, 379, 1998.
16. Evans, M.J., and Kaufman, M.H. Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154, 1981.
17. Martin, G.R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by te-ratocarcinoma stem cells. Proc Natl Acad Sci USA 78, 7634, 1981.
18. Thomson,J.A.,Itskovitz-Eldor,J.,Shapiro,S.S.,Waknitz,M.A., Swiergiel, J.J., Marshall, V.S., and Jones, M. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145, 1998.
19. Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., Soreq, H., and Benvenisty, N. Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. Mol Med 6, 88, 2000.
20. Desbailltes, I., Ziegler, U., Groscurth, P., and Gassmann, M. Embryoid bodies: in an in vitro model of mouse embryo-genesis. Exp Physiol 85, 645, 2000.
21. Cameron, C.M., Harding, F., Hu, W., and Kaufman, D.S. Activation of hypoxic response in human embryonic stem cell-derived embryonic bodies. Exp Biol Med online June 5, 2005. doi:10.3181=0709-RM-263.
22. Vallier, L., and Pederson, R.A. An in vitro model to study mechanisms controlling pluripotency in early mammalian development. Hum Embryonic Stem Cells Stem Cell Rev 1, 119, 2005.
23. Kurosawa, H. Methods of inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. J Biosci Bioeng Rev 103, 389, 2007.
24. Come, J., Nissan, X., Aubry, L., Tournois, J., Girard, M., Perrier, A.L., Peschanski, M., and Cailleret, M. Improvement of culture conditions of human embryoid bodies using a controlled perfused and dialyzed bioreactor system. Tissue Eng C Methods 14, 289, 2008.
25. Hwang, Y.S., Cho, J., Tay, F., Heng, J.Y.Y., Ho, R., Kazarian, S.G., Williams, D.R., Boccaccini, A.R., Polak, J.M., and Mantalaris, A. The use of murine embryonic stem cells, al-ginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering. Biomaterials 30, 499, 2009.
26. Li, Y., Kniss, D.A., Lasky, L.C., and Yang, S.T. Culturing and differentiation of murine embryonic stem cells in a three-dimensional fibrous matrix. Cytotechnology 41, 23, 2003.
27. Ouyang, A., and Yang, S.T. A two-stage perfusion fibrous bed bioreactor system for mass production of embryonic stem cells. Expert Opin Biol Ther 8, 895, 2008.
28. Abranches, E., Bekman, E., Henrique, D., and Cabral, J.M.S. Expansion of mouse embryonic stem cells on microcarriers. Biotechnol Bioeng 96, 1211, 2007.
29. Fernandes, A.M., Fernandes, T.G., Diogo, M.M., da Silva, C.L., Henrique, D., and Cabral, J.M.S. Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system. J Biotechnol 132, 227, 2007.
30. zur Nieden, N.I., Cormier, J.T., Rancourt, D.E., and Kallos, M.S. Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactors. J Biotechnol 129, 421, 2007.
31. Phillips, B.W., Horne, R., Lay, T.S., Rust, W.L., Teck, T.T., Crook, J.M. Attachment and growth of human embryonic stem cells on microcarriers. J Biotechnol 138, 24, 2008.
32. Nie, Y., Bergendahl, V., Hei, D.L., Jones, M.J., and Palecek, S.P. Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Biotechnol Prog 25, 20, 2009.
33. Fong, W.J., Tan, H.L., Choo, A., and Oh, S.K. Perfusion cultures of human embryonic stem cells. Bioprocess Biosyst Eng 27, 381, 2005.
34. Ouyang, A., and Yang, S.T. A two-stage perfusion fibrous bed bioreactor system for mass production of embryonic stem cells. Expert Opin Biol Ther 8, 895, 2008.