Structural and phylogenetic analysis of a novel ζ-carotene desaturase from Dunaliella bardawil, a unicellular alga that accumulates large amounts of β-carotene

Limnology and Oceanography

Volumn 56, Issue 1, 2011, Pages 133-138

  Ye, Zhi Wei ^a   Liu, Guan Nan ^a   Jianga, Jian Guo ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CAROTENOID; CHLOROPLAST; ENZYME ACTIVITY; GREEN ALGA; PHYLOGENETICS; POLYMERASE CHAIN REACTION; PROTEIN;

ALGAE; CHLOROPHYTA; DUNALIELLA; DUNALIELLA BARDAWIL; EMBRYOPHYTA;

EID: 78651410962     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.2011.56.1.0133     Document Type: Article

References (31)

1. ALBRECHT, V., A. KLEIN, P. HUGUENEY, G. SANDMANN, AND M. KUNTZ. 1995. Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating ζ-carotene desaturation. FEBS Lett. 372: 199-202, doi:10.1016/0014-5793(95)00978-I
2. BARTLEY, G. E., AND P. A. SCOLNIK. 1994. Molecular biology of carotenoid biosynthesis in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 287-301, doi:10.1146/annurev. pp.45.060194.001443
3. BATES, P. A., L. A. KELLEY, AND R. M. MACCALLUM. 2001. Enhancement of protein modeling by human intervention in applying the automatic programs 3D-JIGSAW and 3DPSSM. Proteins 5: 39-46, doi:10.1002/prot.1168
4. BEN-AMOTZ, A., A. KATZ, AND M. AVRON. 1982. Accumulation of β-carotene in halotolerant algae: Purification and characterization of β-carotene-rich globules from Dunaliella bardawil (Chlorophyceae). J. Phycol. 18: 529-537, doi:10.1111/j. 1529-8817.1982.tb03219.x
5. BENDTSEN, J. D., H. NIELSEN, G. VON HEIJNE, AND S. BRUNAK. 2004. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340: 783-795, doi:10.1016/j.jmb.2004.05.028
6. BREITENBACH, J., B. FERNÁNDEZ-GONZÁLEZ, A. VIOQUE, AND G. SANDMANN. 1998. A higher-plant type zeta-carotene desaturase in the cyanobacterium Synechocystis PCC6803. Plant Mol. Biol. 36: 725-732, doi:10.1023/A:1005997405431
7. CAMACHO, C., G. COULOURIS, V. AVAGYAN, N. MA, J. PAPADOPOULOS, K. BEALER, AND T. L. MADDEN. 2009. BLAST+: Architecture and applications. BMC Bioinformatics 10: 421, doi:10.1186/1471-2105-10-421
8. DONG, H., AND OTHERS. 2007. The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling. Cell Res. 17: 458-470.
9. GASTEIGER, E., A. GATTIKER, C. HOOGLAND, I. IVANYI, R. D. APPEL, AND A. BAIROCH. 2003. ExPASy: The proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 31: 3784-3788, doi:10.1093/nar/gkg563
10. GASTEIGER, E., A. GATTIKER, C. HOOGLAND, I. IVANYI, R. D. APPEL, AND A. BAIROCH. C. HOOGLAND, A. GATTIKER, S. DUVAUD, M. R. WILKINS, R. D. APPEL, AND A. BAIROCH. 2005. Protein identification and analysis tools on the ExPASy server, p. 571-607. In M. W. John [ed.], The proteomics protocols handbook. Humana Press.
11. HORTON, P., K. J. PARK, T. OBAYASHI, N. FUJITA, H. HARADA, C. J. ADAMS-COLLIER, AND K. NAKAI. 2007. WoLF PSORT: Protein localization predictor. Nucleic Acids Res. 35: W585-W587, doi:10.1093/nar/gkm259
12. JUST, B. J., C. A. SANTOS, M. E. FONSECA, L. S. BOITEUX, B. B. OLOIZIA, AND P. W. SIMON. 2007. Carotenoid biosynthesis structural genes in carrot (Daucus carota): Isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping. Theor. Appl. Genet. 114: 693-704, doi:10.1007/s00122-006-0469-x
13. KYTE, J., AND R. F. DOOLITTLE. 1982. Simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105-132, doi:10.1016/0022-2836(82)90515-0
14. MARCHLER-BAUER, A., J. B. ANDERSON, F. CHITSAZ, M. K. DERBYSHIRE, C. DEWEESE-SCOTT, AND J. H. FONG. 2009. CDD: Specific functional annotation with the conserved domain database. Nucleic Acids Res. 37: D205-D210, doi:10.1093/nar/gkn845
15. MARCHLER-BAUER, A., J. B. ANDERSON, F. CHITSAZ, M. K. DERBYSHIRE, C. DEWEESE-SCOTT, AND J. H. FONG. AND S. H. BRYANT. 2004. CD-Search: Protein domain annotations on the fly. Nucleic Acids Res. 32: W327-W331, doi:10.1093/nar/ gkh454
16. MATTHEWS, P. D., R. LUO, AND E. T. WURTZEL. 2003. Maize phytoene desaturase and zeta-carotene desaturase catalyse a poly-Z desaturation pathway: Implications for genetic engineering of carotenoid content among cereal crops. J. Exp. Bot. 54: 2215-2230, doi:10.1093/jxb/erg235
17. MERCHANT, S. S., AND OTHERS. 2007. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318: 245-250, doi:10.1126/science.1143609
18. MÖLLER, S., M. D. CRONING, AND R. APWEILER. 2001. Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 17: 646-653, doi:10.1093/bioinformatics/17.7.646
19. NISHINO, H., M. MURAKOSHI, H. TOKUDA, AND Y. SATOMI. 2009. Cancer prevention by carotenoids. Arch. Biochem. Biophys. 483: 165-168, doi:10.1016/j.abb.2008.09.011
20. OFOSU, I. W., E. APPIAH-NKANSAH, L. OWUSU, F. B. APEA-BAH, I. ODURO, AND W. O. ELLIS. 2010. Formulation of annatto feed concentrate for layers and the evaluation of egg yolk color preference of consumers. J. Food Biochem. 34: 66-77, doi:10.1111/j.1745-4514.2009.00264.x
21. RAJA, R., S. HEMAISWARYA, AND R. RENGASAMY. 2007. Exploitation of Dunaliella for β-carotene production. Appl. Microbiol. Biotechnol. 74: 517-523, doi:10.1007/s00253-006-0777-8
22. SANDMANN, G. 2009. Evolution of carotene desaturation: The complication of a simple pathway. Arch. Biochem. Biophys. 483: 169-174, doi:10.1016/j.abb. 2008.10.004
23. SAYLE, R. A., AND E. J. MILNER-WHITE. 1995. RASMOL: Biomolecular graphics for all. Trends Biochem. Sci. 20: 374-376, doi:10.1016/S0968-0004(00)89080-5
24. SHAISH, A., A. HARARI, L. HANANSHVILI, H. COHEN, R. BITZUR, AND T. LUVISH. 2006. 9-cis β-carotene-rich powder of the alga Dunaliella bardawil increases plasma HDL-cholesterol in fibrate-treated patients. Atherosclerosis 189: 215-221, doi:10.1016/j.atherosclerosis.2005.12.004
25. SHEFFER, M., AND M. AVRONA. 1986. Isolation of the plasmamembrane of the halotolerant alga Dunaliella salina using sulforhodamine B as a probe. Biochim. Biophys. Acta 857: 155-164, doi:10.1016/0005-2736(86)90343-3
26. TAMURA, K., J. DUDLEY, M. NEI, AND S. KUMAR. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599, doi:10.1093/ molbev/msm092
27. YAN, Y., Y. H. ZHU, J. G. JIANG, AND D. L. SONG. 2005. Cloning and sequence analysis of the phytoene synthase gene from a unicellular chlorophyte, Dunaliella salina. J. Agric. Food Chem. 53: 1466-1469, doi:10.1021/jf048358s
28. YE, Z. W., J. G. JIANG, AND G. H. WU. 2008. Biosynthesis and regulation of carotenoids in Dunaliella: progresses and prospects. Biotechnol. Adv. 26: 352-360, doi:10.1016/j. biotechadv.2008.03.004
29. ZHU, Y. H., J. G. JIANG, AND Q. CHEN. 2008. Characterization of cDNA of lycopene beta-cyclase responsible for a high level of beta-carotene accumulation in Dunaliella salina. Biochem. Cell Biol. 86: 285-292, doi:10.1139/O08-012
30. ZHU, Y. H., J. G. JIANG, AND Q. CHEN, J. G. JIANG, AND Z. X. CHEN. 2007. cDNA for phytoene desaturase in Dunaliella salina and its expressed protein as indicators of phylogenetic position of the β-carotene biosynthetic pathway. J. Sci. Food Agric. 87: 1772-1777, doi:10.1002/jsfa.2922
31. ZHU, Y. H., J. G. JIANG, AND Q. CHEN, J. G. JIANG, AND Z. X. CHEN, Y. YAN, AND X. W. CHEN. 2005. Isolation and characterization of phytoene desaturase cDNA involved in the beta-carotene biosynthetic pathway in Dunaliella salina. J. Agric. Food Chem. 53: 5593-5597, doi:10.1021/jf0506838