Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation

Journal of Molecular Catalysis B: Enzymatic

Volumn 68, Issue 3-4, 2011, Pages 286-291

  Gao, Bei ^a   Xu, Tao ^a   Lin, Jinping ^a   Zhang, Lujia ^a   Su, Erzheng ^a   Jiang, Zhengbing ^b   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HUBEI UNIVERSITY   (China)

Author keywords

1 Phenylethanol; Computer simulation; Lid; LipK107; Site directed mutagenesis

Indexed keywords

1-PHENYLETHANOL; ACYL DONORS; CATALYTIC ACTIVITY; CATALYTIC REACTIONS; COMPUTER MODELS; ENANTIODISCRIMINATION; HIGH ACTIVITY; KINETIC RESOLUTION; LID; LIPASE ACTIVITY; LIPK107; METHYL PALMITATE; MODEL REACTIONS; ORGANIC MEDIUM; SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; CATALYSIS; CATALYST ACTIVITY; COMPUTATIONAL METHODS; ENANTIOSELECTIVITY; ETHANOL; LIPASES; MODEL STRUCTURES; MUTAGENESIS; WATER CONTENT;

COMPUTER SIMULATION;

1 PHENYLETHANOL; MUTANT PROTEIN; PALMITIC ACID METHYL ESTER; TRIACYLGLYCEROL LIPASE; WATER;

ARTICLE; CATALYSIS; CATALYST; CHEMICAL MODIFICATION; COMPUTER MODEL; COMPUTER SIMULATION; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME STRUCTURE; HYDROPHOBICITY; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEUS; RACEMIC MIXTURE; SITE DIRECTED MUTAGENESIS; WATER CONTENT;

PROTEUS SP.;

EID: 78651393065     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2010.12.001     Document Type: Article

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