Short-term corneal response to cross-linking in rabbit eyes assessed by in vivo confocal laser scanning microscopy and histology

Cornea

Volumn 30, Issue 2, 2011, Pages 196-203

  Hovakimyan, Marine ^a   Guthoff, Rudolf ^a   Knappe, Steffi ^a   Zhivov, Andrey ^a   Wree, Andreas ^a   Krüger, Alexander ^b   Heisterkamp, Alexander ^b   Stachs, Oliver ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b LASER ZENTRUM HANNOVER E V   (Germany)

Author keywords

activated keratocytes; confocal microscopy; wound healing

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; CELL DENSITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CORNEA CELL; CORNEA CROSS LINKING; CORNEA EPITHELIUM; CORNEA THICKNESS; CROSS LINKING; EDEMA; ENDOTHELIUM CELL; HISTOLOGY; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; KERATOCONUS; MALE; NONHUMAN; PRIORITY JOURNAL; RABBIT;

ANIMALS; COLLAGEN; CORNEA; CORNEAL STROMA; ENDOTHELIUM, CORNEAL; EPITHELIUM, CORNEAL; KERATOCONUS; MALE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PHOTOCHEMOTHERAPY; PHOTOSENSITIZING AGENTS; RABBITS; RIBOFLAVIN; TIME FACTORS; ULTRAVIOLET RAYS;

EID: 78651376182     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/ICO.0b013e3181e16d93     Document Type: Article

References (32)

1. Rabinowitz YS. Keratoconus. Surv Ophthalmol. 1998;42:297-319.
2. Spoerl E, Seiler T. Techniques for stiffening the cornea. J Refract Surg. 1999;15:711-713.
3. Spoerl E, Wollensak G, Seiler T. Increased resistance of crosslinked cornea against enzymatic digestion. Curr Eye Res. 2004;29:35-40.
4. Wollensak G, Spoerl E, Seiler T. Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking. J Cataract Refract Surg. 2003;29:1780-1785.
5. Wollensak G, Spoerl E, Seiler T. Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus. Am J Ophthalmol. 2003; 135:620-627.
6. Guthoff RF, Stave J. In vivo micromorphology of the cornea: Confocal microscopy principles and clinical applications. In: Reinhard T, Larkin F, eds. Essentials in Ophthalmology: Cornea and External Eye Disease. Berlin, Germany: Springer; 2006:173-208.
7. Mazzotta C, Traversi C, Baiocchi S, et al. Conservative treatment of keratoconus by riboflavin-uva-induced cross-linking of corneal collagen: Qualitative investigation. Eur J Ophthalmol. 2006;16:530-535.
8. Caporossi A, Baiocchi S, Mazzotta C, et al. Parasurgical therapy for keratoconus by riboflavin-ultraviolet type A rays induced cross-linking of corneal collagen: Preliminary refractive results in an Italian study. J Cataract Refract Surg. 2006;32:837-845.
9. Mazzotta C, Balestrazzi A, Traversi C, et al. Treatment of progressive keratoconus by riboflavin-UVA-induced cross-linking of corneal collagen: Ultrastructural analysis by Heidelberg Retinal Tomograph II in vivo confocal microscopy in humans. Cornea. 2007;26:390-397.
10. Mazzotta C, Traversi C, Baiocchi S, et al. Corneal healing after riboflavin ultraviolet-A collagen cross-linking determined by confocal laser scanning microscopy in vivo: Early and late modifications. Am J Ophthalmol. 2008;146:527-533.
11. Kymionis GD, Diakonis VF, Kalyvianaki M, et al. One-year follow-up of corneal confocal microscopy after corneal cross-linking in patients with post laser in situ keratomileusis ectasia and keratoconus. Am J Ophthalmol. 2009;147:774-778.
12. Guerriero E, Chen J, Sado Y, et al. Loss of alpha3(IV) collagen expression associated with corneal keratocyte activation. Invest Ophthalmol Vis Sci. 2007;48:627-635.
13. Karamichos D, Lakshman N, Petroll WM. Regulation of corneal fibroblast morphology and collagen reorganization by extracellular matrix mechanical properties. Invest Ophthalmol Vis Sci. 2007;48: 5030-5037.
14. Erie JC. Corneal wound healing after photorefractive keratectomy: A 3- year confocal microscopy study. Trans Am Ophthalmol Soc. 2003;101: 293-333.
15. Ohno K, Mitooka K, Nelson LR, et al. Keratocyte activation and apoptosis in transplanted human corneas in a xenograft model. Invest Ophthalmol Vis Sci. 2002;43:1025-1031. (Pubitemid 34258270)
16. Ivarsen A, Møller-Pedersen T. LASIK induces minimal regrowth and no haze development in rabbit corneas. Curr Eye Res. 2005;30:363-373.
17. Wollensak G, Spoerl E, Wilsch M, et al. Endothelial cell damage after riboflavin-ultraviolet-A treatment in the rabbit. J Cataract Refract Surg. 2003;29:1786-1790.
18. Wollensak G, Spoerl E,Wilsch M, et al. Keratocyte apoptosis after corneal collagen cross-linking using riboflavin/UVA treatment. Cornea. 2004;23: 43-49.
19. Wollensak G, Iomdina E, Dittert DD, et al. Wound healing in the rabbit cornea after corneal collagen cross-linking with riboflavin and UVA. Cornea. 2007;26:600-605.
20. Denoyer A, Ossant F, Arbeille B, et al. Very-high-frequency ultrasound corneal imaging as a new tool for early diagnosis of ocular surface toxicity in rabbits treated with a preserved glaucoma drug. Ophthalmic Res. 2008; 40:298-308.
21. Hosseini K, Kholodnykh AI, Petrova IY, et al. Monitoring of rabbit cornea response to dehydration stress by optical coherence tomography. Invest Ophthalmol Vis Sci. 2004;45:2555-2562.
22. Petroll WM, Boettcher K, Barry P, et al. Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea. Cornea. 1995;14:3-9.
23. Møller-Pedersen T, Li HF, Petroll WM, et al. Confocal microscopic characterization of wound repair after photorefractive keratectomy. Invest Ophthalmol Vis Sci. 1998;39:487-501.
24. Labbé A, Liang H, Martin C, et al. Comparative anatomy of laboratory animal corneas with a new-generation high-resolution in vivo confocal microscope. Curr Eye Res. 2006;31:501-509.
25. Doughty MJ. The cornea and corneal endothelium in the aged rabbit. Optom Vis Sci. 1994;71:809-818.
26. Khodadoust AA, Green K. Physiological function of regenerating endothelium. Invest Ophthalmol. 1976;15:96-101.
27. Mimura T, Yamagami S, Yokoo S, et al. Comparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea. Invest Ophthalmol Vis Sci. 2005;46:3645-3648.
28. Meltendorf C, Burbach GJ, Bühren J, et al. Corneal femtosecond laser keratotomy results in isolated stromal injury and favorable wound-healing response. Invest Ophthalmol Vis Sci. 2007;48:2068-2075.
29. Ichijima H, Petroll WM, Barry PA, et al. Actin filament organization during endothelial wound healing in the rabbit cornea: Comparison between transcorneal freeze and mechanical scrape injuries. Invest Ophthalmol Vis Sci. 1993;34:2803-2812.
30. Jester JV, Barry PA, Lind GJ, et al. Corneal keratocytes: In situ and in vitro organization of cytoskeletal contractile proteins. Invest Ophthalmol Vis Sci. 1994;35:730-743.
31. West-Mays JA, Dwivedi DJ. The keratocyte: Corneal stromal cell with variable repair phenotypes. Int J Biochem Cell Biol. 2006;38:1625-1631.
32. Nagy ZZ, Tóth J, Nagymihály A, et al. The role of ultraviolet-B in corneal healing following excimer laser in situ keratomileusis. Pathol Oncol Res. 2002;8:41-46.