Structure of human complement C8, a precursor to membrane attack

Journal of Molecular Biology

Volumn 405, Issue 2, 2011, Pages 325-330

  Bubeck, Doryen ^a   Roversi, Pietro ^b   Donev, Rossen ^c   Morgan, B Paul ^c   Llorca, Oscar ^d   Lea, Susan M ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c CARDIFF UNIVERSITY   (United Kingdom)

^d CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

3D electron microscopy; C8; complement; membrane attack complex (MAC); terminal pathway

Indexed keywords

COMPLEMENT COMPONENT C8; COMPLEMENT MEMBRANE ATTACK COMPLEX; PERFORIN;

ARTICLE; CRYSTAL STRUCTURE; ELECTRON MICROSCOPY; HUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE;

EID: 78650896733     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.10.031     Document Type: Article

References (21)

1. Morgan B.P. Physiology and pathophysiology of complement: progress and trends Crit. Rev. Clin. Lab. Sci. 32 1995 265 298
2. Rus H., Cudrici C., and Niculescu F. C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection Ann. Med. 37 2005 97 104
3. Hadders M.A., Beringer D.X., and Gros P. Structure of C8α-MACPF reveals mechanism of membrane attack in complement immune defense Science 317 2007 1552 1554
4. Slade D.J., Lovelace L.L., Chruszcz M., Minor W., Lebioda L., and Sodetz J.M. Crystal structure of the MACPF domain of human complement protein C8α in complex with the C8γ subunit J. Mol. Biol. 379 2008 331 342
5. Lovelace L.L., Chiswell B., Slade D.J., Sodetz J.M., and Lebioda L. Crystal structure of complement protein C8γ in complex with a peptide containing the C8γ binding site on C8α: implications for C8γ ligand binding Mol. Immunol. 45 2008 750 756
6. Slade D.J., Chiswell B., and Sodetz J.M. Functional studies of the MACPF domain of human complement protein C8α reveal sites for simultaneous binding of C8β, C8γ, and C9 Biochemistry 45 2006 5290 5296
7. Brannen C.L., and Sodetz J.M. Incorporation of human complement C8 into the membrane attack complex is mediated by a binding site located within the C8β MACPF domain Mol. Immunol. 44 2007 960 965
8. Meri S., Morgan B.P., Davies A., Daniels R.H., Olavesen M.G., Waldmann H., and Lachmann P.J. Human protectin (CD59), an 18000-20000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers Immunology 71 1990 1 9
9. Lockert D.H., Kaufman K.M., Chang C.P., Husler T., Sodetz J.M., and Sims P.J. Identity of the segment of human complement C8 recognized by complement regulatory protein CD59 J. Biol. Chem. 270 1995 19723 19728
10. Abraha A., Morgan B.P., and Luzio J.P. The preparation and characterization of monoclonal antibodies to human complement component C8 and their use in purification of C8 and C8 subunits Biochem. J. 251 1988 285 292
11. Ohi M., Li Y., Cheng Y., and Walz T. Negative staining and image classification-powerful tools in modern electron microscopy Biol. Proced. Online 6 2004 23 34
12. Ludtke S.J., Baldwin P.R., and Chiu W. EMAN: semiautomated software for high- resolution single-particle reconstructions J. Struct. Biol. 128 1 1999 82 97
13. Radermacher M., Wagenknecht T., Verschoor A., and Frank J. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli J. Microsc. 146 1987 113 136
14. Pettersen E.F., Goddard T.D., Huang C.C., Couch G.S., Greenblatt D.M., Meng E.C., and Ferrin T.E. UCSF Chimera-a visualization system for exploratory research and analysis J. Comput. Chem. 25 2004 1605 1612
15. Rosado C.J. A common fold mediates vertebrate defense and bacterial attack Science 317 2007 1548 1551
16. Eswar N., Webb B., Marti-Renom M.A., Madhusudhan M.S., Eramian D., and Shen M.Y. Comparative protein structure modeling using MODELLER Curr. Protoc. Protein Sci. 2007 2007 Nov; Chapter 2: Unit 2.9
17. Adams P.D., Afonine P.V., Bunkoczi G., Chen V.B., Davis I.W., and Echols N. Acta Crystallogr. Sect. D: Biol. Crystallogr. 66 2010 213 221
18. DeLano W.L. The PyMol Molecular Graphics System 2002 DeLano Scientific San Carlos
19. Ng S.C., Rao A.G., Howard O.M., and Sodetz J.M. The eighth component of human complement: evidence that it is an oligomeric serum protein assembled from products of three different genes Biochemistry 26 1987 5229 5233
20. Musingarimi P., Plumb M.E., and Sodetz J.M. Interaction between the C8 α-γ and C8 β subunits of human complement C8: role of the C8 β N-terminal thrombospondin type 1 module and membrane attack complex/perforin domain Biochemistry 41 2002 11255 11260
21. Rossjohn J., Polekhina G., Feil S.C., Morton C.J., Tweten R.K., and Parker M.W Structures of perfringolysin O suggest a pathway for activation of cholesterol-dependent cytolysins J. Mol. Biol. 367 2007 1227 1236