Improved biohydrogen production with an expression of codon-optimized hemH and lba genes in the chloroplast of Chlamydomonas reinhardtii

Bioresource Technology

Volumn 102, Issue 3, 2011, Pages 2610-2616

  Wu, Shuangxiu ^a   Xu, Lili ^a   Huang, Rui ^a   Wang, Quanxi ^a  

^a SHANGHAI NORMAL UNIVERSITY   (China)

Author keywords

Biohydrogen production; Chlamydomonas reinhardtii; Chloroplast transformation; Codon optimization; Leghemoglobin

Indexed keywords

BIO-HYDROGEN PRODUCTION; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST TRANSFORMATION; CODON OPTIMIZATION; LEGHEMOGLOBIN;

ALGAE; AMINO ACIDS; CELL CULTURE; CHLOROPHYLL; GALLIUM ALLOYS; GENE EXPRESSION;

OPTIMIZATION;

FERROCHELATASE; HEMH PROTEIN; HYDROGEN; LBA PROTEIN; LEGHEMOGLOBIN; OXYGEN; UNCLASSIFIED DRUG;

BIOFUEL; CHLOROPLAST; GENE EXPRESSION; GREEN ALGA; HYDROGEN; OPTIMIZATION; PROTEIN; RESPIRATION;

ALGAL CELL CULTURE; ANAEROBIC CELL CULTURE; ARTICLE; BRADYRHIZOBIUM JAPONICUM; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; CHLOROPLAST GENOME; CODON; CONTROLLED STUDY; GENE EXPRESSION; GENE TRANSFER; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTOZOAL GENETICS; SOYBEAN; TRANSGENIC ORGANISM; UPREGULATION;

BIOFUELS; CHLAMYDOMONAS REINHARDTII; CHLOROPLASTS; CLONING, MOLECULAR; CODON; FERROCHELATASE; GENETIC ENHANCEMENT; HYDROGEN; LEGHEMOGLOBIN;

ALGAE; BRADYRHIZOBIUM JAPONICUM; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; GLYCINE MAX;

EID: 78650844008     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.09.123     Document Type: Article

References (34)

1. Beer L.L., Boyd E.S., Peters J.W., Posewitz M.C. Engineering algae for biohydrogen and biofuel production. Curr. Opin. Biotechnol. 2009, 20:264-271.
2. Boynton J.E., Gillham N.W., Harris E.H., Hosler J.P., Johnson A.M., Jones A.R., Randolph-Anderson B.L., Robertson D., Klein T.M., Shark K.B., Sanford J.C. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 1988, 240:1534-1538.
3. Choi S.P., Nguyen M.T., Sim S.T. Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. Bioresour. Technol. 2010, 101:5330-5336.
4. Dos R.M., Wernisch L., Savva R. Unexpected correlations between gene expression and codon usage bias from microarray data for the whole Escherichia coli K-12 genome. Nucleic Acids Res. 2003, 3l:6976-6985.
5. Downie J.A. Legume haemoglobins: symbiotic nitrogen fixation needs bloody nodules. Curr. Biol. 2005, 15:R196-8.
6. Duret L. TRNA gene number and codon usage in the C. elegans genome are co-adapted for optimal translation of highly expressed genes. Trends Genet. 2000, 16:287-289.
7. Florin L., Tsokoglou A., Happe T. A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain. J. Biol. Chem. 2001, 276:6125-6132.
8. Franklin S., Mayfield S. Prospects for molecular farming in the green alga Chlamydomonas reinhardtii. Curr. Opin. Plant Biol. 2004, 7:159-165.
9. Franklin S., Ngo B., Efurt E., Mayfield S.P. Development of GFP a reporter gene for Chlamydomonas reinhardtii chloroplast. Plant J. 2002, 30:733-744.
10. 2-production. Appl. Biochem. Biotechnol. 1997, 63:141-151.
11. 2. Trends Biotechnol. 2000, 18:506-511.
12. Goldschmidt-Clermont M. Transgenic expression of aminoglycoside adenyl transferase in the chloroplast: a selectable marker for site-directed transformation of Chlamydomonas. Nucleic Acids Res. 1991, 19:4083-4089.
13. Gustafsson C., Govindarajan S., Minshull J. Codon bias and heterologous protein expression. Trends Biotechno1. 2004, 22:346-353.
14. Harris E.H. The Chlarmydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use 1989, Academic Press, Inc., New York.
15. Hemschemeier A., Fouchard S., Cournac L., Peltier G., Happe T. Hydrogen production by Chlamydomonas reinhardtii: an elaborate interplay of electron sources and sinks. Planta 2008, 227:397-407.
16. Kanaya S., Yamada Y., Kinouchi M., Kudo Y., Ikemura T. Codon usage and tRNA genes in eukaryotes: correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage as assessed by multivariate analysis. J. Mol. Evol. 2001, 53:290-298.
17. Lavner Y., Kotlar D. Codon bias as a factor in regulating expression via translation rate in the human genome. Gene 2005, 345:127-138.
18. Liu Q., Dou S., Ji Z., Xue Q. Synonymous codon usage and gene function are strongly related in Oryza sativa. Biosystems 2005, 80:123-131.
19. Makarova V.V., Kosourov S., Krendeleva T.E., Semin B.K., Kukarskikh G.P., Rubin A.B., Sayre R.T., Ghirardi M.L., Seibert M. Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity. Photosynth. Res. 2007, 94:79-89.
20. Mayfield S., Schultz J. Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast. Plant J. 2003, 37:449-458.
21. Mayfield S., Flanklin S., Lerner R. Expression and assembly of a fully active antibody in algae. Proc. Natl. Acad. Sci. USA 2004, 100:438-442.
22. 2 metabolism in Chlamydomonas reinhardtii. Planta 2007, 226:1075-1086.
23. Melis A., Zhang L., Forestier M., Ghirardi M.L., Seibert M. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 2000, 122:127-136.
24. Morowvat M.H., Rasoul-Amini S., Ghasemi Y. Chlamydomonas as a "new" organism for biodiesel production. Bioresour. Technol. 2010, 101:2059-2062.
25. Nakamura Y., Gojobori T., Ikemura T. Codon usage tabulated from international DNA sequence databases. Nucleic Acids Res. 2000, 28:292.
26. Nguyen A.V., Thomas-Hall S.R., Malnoë A., Timmins M., Mussgnug J.H., Rupprecht J., Kruse O., Hankamer B., Schenk P.M. Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green alga Chlamydomonas reinhardtii. Eukaryotic Cell 2008, 7:1965-1979.
27. O'Brain M.R., Kirshbom P.M., Maier R.J. Bacterial heme synthesis is required for expression of the leghemoglobin holoprotein but not apoprotein in soybean root nodules. Proc. Natl. Acad. Sci. USA 1987, 84:8390-8393.
28. Rochaix J.D., Erickson J. Function and assembly of photosystem II: genetic and molecular analysis. Trends Biochem. Sci. 1988, 13:56-59.
29. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York.
30. Vaistij F.E., Goldschmidt-Clermont M., Wostrikoff K., Rochaix J. Stability determinants in the chloroplast psbB/T/H mRNAs in Chlamydomonas reinhardtii. Plant J. 2000, 21:469-482.
31. Woykoff D.D., Davies J.P., Melis A., Grossman A.R. The relationship of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 1998, 117:129-139.
32. Wu S.X., Hang R., Xu L.L., Yan G.Y., Wang Q.X. Improvement of hydrogen production with expression of hemH gene and lba gene in chloroplast of Chlamydomonas reinhardtii. J. Biotechnol. 2010, 146:120-125.
33. Wu, S.X., Yan, G.Y., Xu, L.L., Wang, Q.X., Liu, X.L., in press. Improvement of hydrogen production with expression of lba gene from soybean in chloroplast of Chlamydomonas reinhardtii. Int. J. Hydrogen Energy. doi:10.1016/j.ijhydene.2009.11.118.
34. Yan G.Y., Liu X.L., Wang Q.X., Wu S.X. Cloning and transformation of lba gene of Glycine max into chloroplast Chlamydomonas reinhardtii. China Biotechnol. 2009, 29:66-71.