Application of somatic embryos to rapid and reliable analysis of soybean seed components by RNA interference-mediated gene silencing

Plant Biotechnology

Volumn 27, Issue 5, 2010, Pages 409-420

  Nishizawa, Keito ^a,b   Takagi, Kyoko ^a   Teraishi, Masayoshi ^c   Kita, Akiko ^a   Ishimoto, Masao ^a,d  

^a NATIONAL AGRICULTURAL RESEARCH CENTER FOR HOKKAIDO REGION   (Japan)

^b NATIONAL INSTITUTE OF CROP SCIENCE   (Japan)

^c KYOTO UNIVERSITY   (Japan)

^d NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

RNA interference; Somatic embryo; Soybean (Glycine max); Transformation; Conglycinin

Indexed keywords

GLYCINE MAX;

EID: 78650778293     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.10.0823a     Document Type: Article

References (73)

1. Baulcombe DC (1999) Fast forward genetics based on virus- induced gene silencing. Curr Opin Plant Biol 2: 109-113
2. Birt DF, Hendrich S, Alekel DL, Anthony M (2004) Soybean and the prevention of chronic human disease. In: Boerma RH, Specht JE (eds) Soybeans: Improvement, Production, and Uses, Ed 3. American Society of Agronomy, Madison, WI, pp 1047-1117
3. Boerma HR, Walker DR (2005) Discovery and utilization of QTLs for insect resistance in soybean. Genetica 123: 181-189
4. Cahoon EB, Carlson TJ, Ripp KG, Schweiger BJ, Cook GA, Hall SE, Kinney AJ (1999) Biosynthetic origin of conjugated double bonds: production of fatty acid components of high-value drying oils in transgenic soybean embryos. Proc Natl Acad Sci USA 96 12935-12940
5. Cahoon EB, Marillia EF, Stecca KL, Hall SE, Taylor DC, Kinney AJ (2000) Production of fatty acid components of meadowfoam oil in somatic soybean embryos. Plant Physiol 124: 243-251
6. Cahoon EB, Ripp KG, Hall SE, McGonigle B (2002) Transgenic production of epoxy fatty acids by expression of a cytochrome P450 enzyme from Euphorbia lagascae seed. Plant Physiol 128: 615-624
7. Chen R, Matsui K, Ogawa M, Oe M, Ochiai M, Kawashima H, Sakuradani E, Shimizu S, Ishimoto M, Hayashi M, et al. (2006) Expression of Δ6, Δ5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean [Glycine max (L.) Merrill] seeds. Plant Sci 170: 399-406
8. Dahmer ML, Collins GB, Hildebrand DF (1991) Lipid content and composition of soybean somatic embryos. Crop Sci 31: 741-746
9. Dahmer ML, Hildebrand DF, Collins GB (1992) Comparative protein accumulation patterns in soybean somatic and zygotic embryos. In Vitro Cell Dev Biol 28P: 106-114
10. Dai S, Zheng P, Marmey P, Zhang S, Tian W, Chen S, Beachy RN, Fauquet C (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol Breed 7: 25-33
11. Derbyshire E, Wright DB, Boulter D (1976) Legumin and vicilin, storage proteins of legume seeds. Phytochemistry 15: 3-24
12. Doyle JJ, Schuler MA, Godette WD, Zenger V, Beachy RN, Slightom JL (1986) The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. Structural homologies of genes and proteins. J Biol Chem 261: 9228-9238
13. El-Shemy HA, Teraishi M, Khalafalla MM, Katsube-Tanaka T, Utsumi S, Ishimoto M (2004) Isolation of soybean plants with stable transgene expression by visual selection based on green fluorescent protein. Mol Breed 14: 227-238
14. Finer JJ, Mcmullen MD (1991) Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. In Vitro Cell Dev Biol 27: 175-182
15. Finer JJ, Nagasawa A (1988) Development of an embryogenic suspension culture of soybean (Glycine max Merr) Plant Cell Tissue Organ Cult 15: 125-136
16. Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper DA, Nguyen HT, Zhang ZJ (2008) Silencing of GmFAD3 gene by siRNA leads to low a-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr)]. Transgenic Res 17: 839-850
17. Goto K, Kanazawa A, Kusaba M, Masuta C (2003) A simple and rapid method to detect plant siRNAs using nonradioactive probes. Plant Mol Biol Rep 21: 51-58
18. Graham TL, Graham MY, Subramanian S, Yu O (2007) RNAi silencing of genes for elicitation or biosynthesis of 5-deoxyisoflavonoids suppresses race-specific resistance and hypersensitive cell death in Phytophthora sojae infected tissues. Plant Physiol 144: 728-740
19. Hajika M, Takahashi M, Sakai S, Igita K (1996) A new genotype of 7S globulin (β-Conglycinin) detected in wild soybean (Glycine soja Sieb. et Zucc.). Breed Sci 46: 385-386
20. Hajika M, Takahashi M, Sakai S, Matsunaga R (1998) Dominant inheritance of a trait lacking β-Conglycinin detected in a wild soybean line. Breed Sci 48: 383-386
21. Harada JJ, Barker SJ, Goldberg RB (1989) Soybean β-Conglycinin genes are clustered in several DNA regions and are regulated by transcriptional and posttranscriptional processes. Plant Cell 1: 415-425
22. Hayashi M, Harada K, Fujiwara T, Kitamura K (1998) Characterization of a 7S globulin-deficient mutant of soybean (Glycine max (L.) Merr). Mol Gen Genet 258: 208-214
23. Herman EM, Helm RM, Jung R, Kinney AJ (2003) Genetic modification removes an immunodominant allergen from soybean. Plant Physiol 132: 36-43
24. Hirai S, Oka S, Adachi E, Kodama H (2007) The effects of spacer sequences on silencing effeciency of plant RNAi vectors. Plant Cell Rep 26: 651-659
25. Ishihara S, Yamamoto Y, Ifuku I, Sato F (2005) Functional analysis of four members of the psbP family in Photosystem II in Nicotiana tabacum using differential RNA Interference. Plant Cell Physiol 46: 1885-1893
26. Kachroo A, Fu D, Havens W, Navarre D, Kachroo P, Ghabrial SA (2008) An oleic acid-mediated pathway induces constitutive defense signaling and enhanced resistance to multiple pathogens in soybean. Mol Plant Microbe Interact 21: 564-575
27. Karimi M, Inze D, Depicker A (2002) GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci 7: 193-195
28. Khalafalla MM, Rhaman SM, El-Shemy HA, Nakamoto Y, Wakasa K, Ishimoto M (2005) Optimization of particle bombardment conditions by monitoring of transient sGFP(S65T) expression in transformed soybean. Breed Sci 55: 257-263
29. Kita Y, Nishizawa K, Takahashi M, Kitayama M, Ishimoto M (2007) Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines. Plant Cell Rep 26: 439-447
30. Kitagawa S, Ishimoto M, Kikuchi F, Kitamura K (1991) A characteristic lacking or remarkably decreasing 7S globulin subunits induced with gamma-ray irradiation in soybean seeds. Jpn J Breed 41 (Supp 2): 460-461
31. Kitamura Y, Arahira M, Itoh Y, Fukazawa C (1990) The complete nucleotide sequence of soybean glycinin A2B1a gene spanning to another glycinin gene A1aB1b. Nucleic Acids Res 18: 4245
32. Ko TS, Korban SS, Somers DA (2006) Soybean (Glycine max) transformation using immature cotyledon explants. Meth Mol Biol 343: 397-405
33. Kohli A, Gahakwa D, Vain P, Laurie DA, Christou P (1999) Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing. Planta 208: 88-97
34. Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95: 7203-7208
35. Lawrence RJ, Pikaard CS (2003) Transgene-induced RNAinterference: a strategy for overcoming gene redundancy in polyploids to generate loss-of-function mutations. Plant J 36: 114-121
36. Lazzeri PA, Hildebrand DF, Collins GB (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol Biol Rep 3: 160-167
37. Lee MY, Shin KH, Kim YK, Suh JY, Gu YY, Kim MR, Hur YS, Son O, Kim JS, Song E, et al. (2005) Induction of thioredoxin is required for nodule development to reduce reactive oxygen species levels in soybean roots. Plant Physiol 139: 1881-1889
38. Li J, Todd TC, Trick HN (2010) Rapid in planta evaluation of root expressed transgenes in chimeric soybean plants. Plant Cell Rep 29: 113-123
39. Liu B, Watanabe S, Uchiyama T, Kong F, Kanazawa A, Xia Z, Nagamatsu A, Arai M, Yamada T, Kitamura K, et al. (2010) Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1. Plant Physiol 153: 198-210
40. Miki D, Itoh R, Shimamoto K (2005) RNA silencing of single and multiple members in a gene family of rice. Plant Physiol 138: 1903-1913
41. Miki D, Shimamoto K (2004) Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant Cell Physiol 45: 490-495
42. Mori T, Maruyama N, Nishizawa K, Higasa T, Yagasaki K, Ishimoto M, Utsumi S (2004) The composition of newly synthesized proteins in the endoplasmic reticulum determines the transport pathways of soybean seed storage proteins. Plant J 40: 238-249
43. Nagamatsu A, Masuta C, Senda M, Matsuura H, Kasai A, Hong JS, Kitamura K, Abe J, Kanazawa A (2007) Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. Plant Biotechnol J 5: 778-790
44. Nishizawa K, Ishimoto M (2009) Maturation of somatic embryos as a model for soybean seed development. Plant Biotechnol 26: 543-550
45. Nishizawa K, Kita Y, Kitayama M, Ishimoto M (2006) A red fluorescent protein, DsRed2, as a visual reporter for transient expression and stable transformation in soybean. Plant Cell Rep 25: 1355-1361
46. Nishizawa K, Maruyama N, Satoh R, Fuchikami Y, Higasa T, Utsumi S (2003) A C-terminal sequence of soybean β-Conglycinin a subunit acts as a vacuolar sorting determinant in seed cells. Plant J 34: 647-659
47. Nunes AC, Vianna GR, Cuneo F, Amaya-Farfan J, de Capdeville G, Rech EL, Aragao FJ (2006) RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene(GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. Planta 224: 125-132
48. Ogawa T, Bando N, Tsuji H, Nishikawa K, Kitamura K (1995) a-subunit of β-Conglycinin, an allergenic protein recognized by IgE antibodies of soybean-sensitive patients with atopic dermatitis. Biosci Biotechnol Biochem 59: 831-833
49. Olhoft PM, Donovan CM, Somers DA (2006) Soybean (Glycine max) transformation using mature cotyledonary node explants. Met Mol Biol 343: 385-396
50. Olhoft PM, Flagel LE, Donovan CM, Somers DA (2003) Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. Planta 216: 723-735
51. Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) Optimization of somatic embryogenesis and embryo germination in soybean. In Vitro Cell Dev Biol 24: 817-820
52. Ranch JP, Oglesby L, Zielinski AC (1985) Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro Cell Dev Biol 21: 653-658
53. Samoylov VM, Tucker DM, Thibaud-Nissen F, Parrott WA (1998) A liquid-medium-based protocol for rapid regeneration from embryogenic soybean cultures Plant Cell Rep 18: 49-54
54. Sato F (2005) RNAi and functional genomics. Plant Biotechnol 22: 431-442
55. Sato H, Yamada T, Kita Y, Ishimoto M, Kitamura K (2007) Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka. Plant Biotechnol 24: 535-536
56. Sato S, Newell C, Kolacz K, Tredo L, Finer J, Hinchee M (1993) Stable transformation via particle bombardment in two different soybean regeneration systems. Plant Cell Rep 12: 408-413
57. Schmidt MA, Herman EM (2008) Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes. Molecular Plant 1: 910-924
58. Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, et al. (2010) Genome sequence of the palaeopolyploid soybean. Nature 463: 178-183
59. Sebastiani FL, Farrell LB, Schuler MA, Beachy RN (1990) Complete sequence of a cDNA of a subunit of soybean β-Conglycinin. Plant Mol Biol 15: 197-201
60. Senda M, Masuta C, Ohnishi S, Goto K, Kasai A, Sano T, Hong JS, MacFarlane S (2004) Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes. Plant Cell 16: 807-818
61. Shi J, Wang H, Schellin K, Li B, Faller M, Stoop JM, Meeley RB, Ertl DS, Ranch JP, Glassman K (2007) Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds. Nature Biotechnol 25: 930-937
62. Shoemaker RC, Hammond EG (1988) Fatty acid composition of soybean (Glycine max (L.) Merr.) somatic embryos. In Vitro Cell Dev Biol 24: 829-832
63. Shou H, Frame BR, Whitham SA, Wang K (2004) Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation. Mol Breed 13: 201-208
64. Stacey G, Vodkin L, Parrott WA, Shoemaker RC (2004) National Science Foundation-sponsored workshop report. Draft plan for soybean genomics. Plant Physiol 135: 59-70
65. Subramanian S, Graham MY, Yu O, Graham TL (2005) RNA interference of soybean isoflavone synthase genes leads to silencing in tissues distal to the transformation site and to enhanced susceptibility to Phytophthora sojae. Plant Physiol 137: 1345-1353
66. Teraishi M, Takahashi M, Hajika M, Matsunaga R, Uematsu Y, Ishimoto M (2001) Suppression of soybean β-Conglycinin genes by a dominant gene, Scg-1. Theor Appl Genet 103: 1266-1272
67. Travella S, Klimm TE, Keller B (2006) RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat. Plant Physiol 142: 6-20
68. Walker DR, Parrott WA (2001) Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion. Plant Cell Tissue Organ Cult 64: 55-62
69. Wang G, Xu Y (2008) Hypocotyl-based Agrobacterium-mediated transformation of soybean (Glycine max) and application for RNA interference. Plant Cell Rep 27: 1177-1184
70. Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, et al. (2001) Construct design for efficient, effective and highthroughput gene silencing in plants. Plant J 27: 581-590
71. Yamagishi N, Yoshikawa N (2009) Virus-induced gene silenicng in soybean seeds and the emergence stage of soybean plants with Apple latent spherical virus vector. Plant Mol Biol 71: 15-24
72. Zeng P, Vadnais DA, Zhang Z, Polacco JC (2004) Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 22: 478-482
73. Zhang C, Ghabrial SA (2006) Development of bean pod mottle virus-based vectors for stable protein expression and sequence- specific virus-induced gene silencing in soybean. Virology 344: 401-411.