Effects of site-directed mutagenesis of the loop residue of the N-terminal domain Gly117 of thermolysin on its catalytic activity

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 12, 2010, Pages 2457-2462

  Menach, Evans ^a   Yasukawa, Kiyoshi ^a   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Activity; Glycine; Metalloproteinase; Sitedirected mutagenesis; Thermolysin

Indexed keywords

ACTIVITY; GLYCINE; METALLOPROTEINASES; SITE DIRECTED MUTAGENESIS; THERMOLYSIN;

AMIDES; AMINO ACIDS; ENZYME ACTIVITY; ESCHERICHIA COLI; ESTERS; HYDROLYSIS; MUTAGENESIS; POLYPEPTIDES;

CATALYST ACTIVITY;

ACRYLIC ACID DERIVATIVE; DIPEPTIDE; GLYCINE; N (3 (2 FURYL)ACRYLOYL)GLYCYL LEUCINAMIDE; N BENZYLOXYCARBONYL ASPARTYLPHENYLALANINE METHYL ESTER; N-(3-(2-FURYL)ACRYLOYL)GLYCYL-LEUCINAMIDE; N-BENZYLOXYCARBONYL-ASPARTYLPHENYLALANINE METHYL ESTER; THERMOLYSIN;

ANIMAL; ARTICLE; BIOCATALYSIS; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME STABILITY; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

ACRYLATES; ANIMALS; BIOCATALYSIS; CATALYTIC DOMAIN; CATTLE; DIPEPTIDES; ENZYME STABILITY; GLYCINE; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; TEMPERATURE; THERMOLYSIN;

ESCHERICHIA COLI;

EID: 78650665157     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.100536     Document Type: Article

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