Effects of the mutational combinations on the activity and stability of thermolysin

Journal of Biotechnology

Volumn 147, Issue 1, 2010, Pages 7-16

  Kusano, Masayuki ^a   Yasukawa, Kiyoshi ^a   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Activity stability relationship; Mutational combination; Site directed mutagenesis; Thermal stability; Thermolysin

Indexed keywords

HYDROLYTIC ACTIVITIES; METHYL ESTERS; SINGLE MUTATION; SITE DIRECTED MUTAGENESIS; THERMAL INACTIVATION; THERMAL STABILITY; THERMOLYSIN; WILD TYPES;

AMIDES; ESTERS; MUTAGENESIS; RATE CONSTANTS; THERMOGRAVIMETRIC ANALYSIS;

THERMODYNAMIC STABILITY;

CASEIN; N [3 (2 FURYL)ACRYLOYL]GLYCYLLEUCINAMIDE; N CARBOBENZOXY ASPARTYLPHENYLALANINE METHYL ESTER; PEPTIDE DERIVATIVE; THERMOLYSIN; UNCLASSIFIED DRUG; ACRYLIC ACID DERIVATIVE; DIPEPTIDE; MUTANT PROTEIN; N-(3-(2-FURYL)ACRYLOYL)GLYCYL-LEUCINAMIDE; N-BENZYLOXYCARBONYL-ASPARTYLPHENYLALANINE METHYL ESTER; SODIUM CHLORIDE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; ESCHERICHIA COLI; HYDROLYSIS; MUTATION; MUTATIONAL ANALYSIS; MUTATIONAL COMBINATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SUPERNATANT; THERMOSTABILITY; CELL FRACTIONATION; CHEMISTRY; DRUG EFFECTS; ENZYME ACTIVATION; ENZYME SPECIFICITY; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MUTAGENESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ACRYLATES; CASEINS; DIPEPTIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MUTAGENESIS; MUTANT PROTEINS; MUTATION; SODIUM CHLORIDE; SUBCELLULAR FRACTIONS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMOLYSIN; TRANSFORMATION, GENETIC;

EID: 77951978449     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.02.024     Document Type: Article

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