Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1784, Issue 3, 2008, Pages 481-488

  Takita, Teisuke ^a   Aono, Takahiro ^a   Sakurama, Haruko ^a   Itoh, Takafumi ^a   Wada, Takumi ^a   Minoda, Masashi ^b   Yasukawa, Kiyoshi ^a   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Daiwa Kasei K K   (Japan)

Author keywords

Metalloproteinase; Molecular surface; Site directed mutagenesis; Thermal stability; Thermolysin

Indexed keywords

AMINO ACID DERIVATIVE; ASPARTIC ACID; CALCIUM; CALCIUM CHLORIDE; CASEIN; N [3 (2 FURYL)ACRYLOYL]GLYCYLLEUCINAMIDE; SERINE; SODIUM CHLORIDE; THERMOLYSIN;

AMINO ACID SUBSTITUTION; ARTICLE; CALCIUM BINDING; ENTHALPY; ENTROPY; ENZYME ACTIVATION; ENZYME ACTIVITY; INCUBATION TIME; INDUCED MUTATION; MOLECULAR DYNAMICS; MOLECULAR STABILITY; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WILD TYPE;

ACRYLATES; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BACILLUS; BACTERIAL PROTEINS; CALCIUM CHLORIDE; DIPEPTIDES; ENZYME STABILITY; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; SERINE; THERMOLYSIN;

EID: 43049141115     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2007.12.004     Document Type: Article

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