Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity

Journal of Biochemistry

Volumn 141, Issue 6, 2007, Pages 835-842

  Tatsumi, Chika ^a   Hashida, Yasuhiko ^a   Yasukawa, Kiyoshi ^a   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Expression; Metalloproteinase; Site directed mutagenesis; Surface residue; Thermolysin

Indexed keywords

AMIDE; ESTER DERIVATIVE; METALLOPROTEINASE; N [3 (2 FURYL)ACRYLOYL)]GLYCYLLEUCINE AMIDE; N CARBOBENZOXY ASPARTYLPHENYLALANINE METHYL ESTER; THERMOLYSIN; UNCLASSIFIED DRUG; GLUTAMINE; ZINC;

ARTICLE; CATALYSIS; CONTROLLED STUDY; GENE MUTATION; HYDROLYSIS; HYDROPHOBICITY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY; THERMODYNAMICS; THERMOSTABILITY; BACILLUS; BINDING SITE; CHEMISTRY; CONFORMATION; GENETICS; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PH; STEREOISOMERISM; SURFACE PROPERTY; TEMPERATURE;

BACILLUS; BINDING SITES; CATALYSIS; DNA MUTATIONAL ANALYSIS; GLUTAMINE; HYDROGEN-ION CONCENTRATION; KINETICS; METALLOPROTEASES; MOLECULAR CONFORMATION; MUTAGENESIS, SITE-DIRECTED; STEREOISOMERISM; SURFACE PROPERTIES; TEMPERATURE; THERMOLYSIN; ZINC;

EID: 34748868292     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvm087     Document Type: Article

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