Development of a highly sensitive real-time RT-PCR protocol for the detection of Classical swine fever virus independent of the 5' untranslated region

Journal of Virological Methods

Volumn 171, Issue 1, 2011, Pages 314-317

  Leifer, Immanuel ^a   Blome, Sandra ^a   Beer, Martin ^a   Hoffmann, Bernd ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Classical swine fever virus; Confirmatory assay; NS5A genome region; Real time RT PCR

Indexed keywords

BETA ACTIN; VIRUS RNA;

5' UNTRANSLATED REGION; AMPLICON; ARTICLE; DIAGNOSTIC VALUE; DILUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE RESULT; GENOME ANALYSIS; GENOTYPE; IN VITRO STUDY; NONHUMAN; PESTIVIRUS; PESTIVIRUS INFECTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS GENOME; VIRUS ISOLATION;

5' UNTRANSLATED REGIONS; ANIMALS; CLASSICAL SWINE FEVER; CLASSICAL SWINE FEVER VIRUS; DNA PRIMERS; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE; VIRAL NONSTRUCTURAL PROTEINS; VIROLOGY;

CLASSICAL SWINE FEVER VIRUS; PESTIVIRUS; SUIDAE;

EID: 78650533188     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.11.014     Document Type: Article

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