Primers with 5′ flaps improve real-time PCR

BioTechniques

Volumn 43, Issue 6, 2007, Pages 770-774

  Afonina, Irina ^a,b   Ankoudinova, Irina ^a   Mills, Alan ^a   Lokhov, Sergey ^a   Huynh, Phan ^a   Mahoney, Walt ^a  

^a Nanogen   (United States)

^b Nanogen Inc Bothell   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING SITES; BIOTECHNOLOGY; DNA; FLUORESCENCE; REAL TIME SYSTEMS;

FLUORESCENT SIGNALS; PRIMERS; RESTRICTION SITES;

ENZYMES;

BISULFITE; VIRUS DNA; FLUORESCENT DYE; HYDROGEN SULFITE; PRIMER DNA; SULFITE;

5' UNTRANSLATED REGION; ARTICLE; AT RICH SEQUENCE; BINDING SITE; CONTROLLED STUDY; DNA SEQUENCE; DNA TEMPLATE; ENTEROVIRUS; FLUORESCENCE; GENE AMPLIFICATION; GENE PROBE; GENE SEQUENCE; GENE TARGETING; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL PROCESSING; VARICELLA ZOSTER VIRUS; DNA FLANKING REGION; GENETICS; HUMAN; METHODOLOGY; REPRODUCIBILITY; RESTRICTION MAPPING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD;

5' FLANKING REGION; DNA PRIMERS; DNA, VIRAL; FLUORESCENT DYES; HUMANS; REPRODUCIBILITY OF RESULTS; RESTRICTION MAPPING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SULFITES;

EID: 37349023983     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112631     Document Type: Article

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