Structure-cytotoxicity relationships in bovine seminal ribonuclease: New insights from heat and chemical denaturation studies on variants

FEBS Journal

Volumn 278, Issue 1, 2011, Pages 111-122

  Giancola, Concetta ^a   Ercole, Carmine ^a   Fotticchia, Iolanda ^a   Spadaccini, Roberta ^b   Pizzo, Elio ^a   D'Alessio, Giuseppe ^a   Picone, Delia ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITY OF SANNIO   (Italy)

Author keywords

calorimetric analysis; chemical denaturation; cytotoxic ribonucleases; domain swapping; structure activity relationships

Indexed keywords

BOVINE SEMINAL RIBONUCLEASE; DIMER; MONOMER; RIBONUCLEASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BIOLOGICAL ACTIVITY; CELL SURVIVAL; CONCENTRATION (PARAMETERS); CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; CYTOTOXICITY; DISSOCIATION; ENZYME ACTIVITY; ENZYME DENATURATION; HYDROGEN BOND; INCUBATION TEMPERATURE; MOLECULAR MODEL; MOLECULAR STABILITY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; PROTEIN VARIANT; STRUCTURE ACTIVITY RELATION; THERMODYNAMICS; TUMOR CELL CULTURE; WILD TYPE;

ANIMALS; BALB 3T3 CELLS; CALORIMETRY, DIFFERENTIAL SCANNING; CATTLE; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ENDORIBONUCLEASES; FIBROBLASTS; HOT TEMPERATURE; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MODELS, MOLECULAR; PROTEIN DENATURATION; STRUCTURE-ACTIVITY RELATIONSHIP; THERMODYNAMICS;

BOVINAE;

EID: 78650490730     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07937.x     Document Type: Article

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