Ethanol Metabolism by HeLa Cells Transduced With Human Alcohol Dehydrogenase Isoenzymes: Control of the Pathway by Acetaldehyde Concentration

Alcoholism: Clinical and Experimental Research

Volumn 35, Issue 1, 2011, Pages 28-38

  Matsumoto, Michinaga ^a   Cyganek, Izabela ^a   Sanghani, Paresh C ^a   Cho, Won Kyoo ^a   Liangpunsakul, Suthat ^a   Crabb, David W ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Acetaldehyde; Enzyme Kinetics; Ethanol Elimination; Product Inhibition; Protein Half Life

Indexed keywords

ACETALDEHYDE; ALCOHOL; ALCOHOL DEHYDROGENASE; ALCOHOL DEHYDROGENASE BETA1; ALCOHOL DEHYDROGENASE BETA2; ALDEHYDE DEHYDROGENASE; ISOENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG;

ALCOHOL METABOLISM; ALCOHOL OXIDATION; ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; HALF LIFE TIME; HELA CELL; HUMAN; LIVER CELL; MALE; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; RAT;

ACETALDEHYDE; ALCOHOL DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; ALLELES; ANIMALS; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; CENTRAL NERVOUS SYSTEM DEPRESSANTS; ETHANOL; HELA CELLS; HEPATOCYTES; HUMANS; ISOENZYMES; LIVER; MALE; NAD; OXIDATION-REDUCTION; RATS; RATS, SPRAGUE-DAWLEY;

RATTUS;

EID: 78650329574     PISSN: 01456008     EISSN: 15300277     Source Type: Journal    
DOI: 10.1111/j.1530-0277.2010.01319.x     Document Type: Article

References (43)

1. Baraona E, Zeballos GA, Shoichet L, Mak KM, Lieber CS (2002) Ethanol consumption increases nitric oxide production in rats, and its peroxynitrite-mediated toxicity is attenuated by polyenylphosphatidylcholine. Alcohol Clin Exp Res 26:883-889.
2. Boren J, Graham L, Wettesten M, Scott J, White A, Olofsson SO (1992) The assembly and secretion of ApoB 100-containing lipoproteins in Hep G2 cells. ApoB 100 is cotranslationally integrated into lipoproteins. J Biol Chem 267:9858-9867.
3. Bosron WF, Crabb DW, Housinger TA, Li TK (1984) Effect of fasting on the activity and turnover of rat liver alcohol dehydrogenase. Alcohol Clin Exp Res 8:196-200.
4. Bosron WF, Li TK (1986) Genetic polymorphism of human liver alcohol and aldehyde dehydrogenases, and their relationship to alcohol metabolism and alcoholism. Hepatology 6:502-510.
5. Bosron WF, Li TK (1987) Catalytic properties of human liver alcohol dehydrogenase isoenzymes. Enzyme 37:19-28.
6. Bucher T, Brauser B, Conze A, Klein F, Langguth O, Sies H (1972) State of oxidation-reduction and state of binding in the cytosolic NADH-system as disclosed by equilibration with extracellular lactate-pyruvate in hemoglobin-free perfused rat liver. Eur J Biochem 27:301-317.
7. Crabb DW, Bosron WF, Li TK (1983) Steady-state kinetic properties of purified rat liver alcohol dehydrogenase: application to predicting alcohol elimination rates in vivo. Arch Biochem Biophys 224:299-309.
8. Crabb DW, Matsumoto M, Chang D, You M (2004) Overview of the role of alcohol dehydrogenase and aldehyde dehydrogenase and their variants in the genesis of alcohol-related pathology. Proc Nutr Soc 63:49-63.
9. Crabb DW, Stewart MJ, Xiao Q (1995) Hormonal and chemical influences on the expression of class 2 aldehyde dehydrogenases in rat H4IIEC3 and human HuH7 hepatoma cells. Alcohol Clin Exp Res 19:1414-1419.
10. Ehrig T, Bosron WF, Li TK (1990) Alcohol and aldehyde dehydrogenase. Alcohol Alcohol 25:105-116.
11. Enomoto N, Takase S, Yasuhara M, Takada A (1991) Acetaldehyde metabolism in different aldehyde dehydrogenase-2 genotypes. Alcohol Clin Exp Res 15:141-144.
12. Galli A, Price D, Crabb D (1999) High-level expression of rat class I alcohol dehydrogenase is sufficient for ethanol-induced fat accumulation in transduced HeLa cells. Hepatology 29:1164-1170.
13. Gergel D, Cederbaum AI (1996) Inhibition of the catalytic activity of alcohol dehydrogenase by nitric oxide is associated with S nitrosylation and the release of zinc. Biochemistry 35:16186-16194.
14. Graham FL, van der Eb AJ (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467.
15. Gritz L, Davies J (1983) Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25:179-188.
16. Hasin D, Aharonovich E, Liu X, Mamman Z, Matseoane K, Carr LG, Li TK (2002) Alcohol dependence symptoms and alcohol dehydrogenase 2 polymorphism: Israeli Ashkenazis, Sephardics, and recent Russian immigrants. Alcohol Clin Exp Res 26:1315-1321.
17. Lamprecht W, Heinz F (1984) Pyruvate, in Methods of Enzymatic Analysis (Bergmeyer HU ed) 3rd edn, pp. 570-577. Verlag Chemie, Basel.
18. Luu SU, Wang MF, Lin DL, Kao MH, Chen ML, Chiang CH, Pai L, Yin SJ (1995) Ethanol and acetaldehyde metabolism in Chinese with different aldehyde dehydrogenase-2 genotypes. Proc Natl Sci Counc Repub China B 19:129-136.
19. Mauch TJ, Donohue TM Jr, Zetterman RK, Sorrell MF, Tuma DJ (1986) Covalent binding of acetaldehyde selectively inhibits the catalytic activity of lysine-dependent enzymes. Hepatology 6:263-269.
20. Miller AD, Rosman GJ (1989) Improved retroviral vectors for gene transfer and expression. BioTechniques 7:980-990.
21. Mizoi Y, Yamamoto K, Ueno Y, Fukunaga T, Harada S (1994) Involvement of genetic polymorphism of alcohol and aldehyde dehydrogenases in individual variation of alcohol metabolism. Alcohol Alcohol 29:707-710.
22. Moon KH, Hood BL, Kim BJ, Hardwich JP, Conrads TP, Veenstra TD, Song BJ (2006) Inactivation of oxidized and s-nitrosylated mitochondrial proteins in alcoholic fatty liver of rats. Hepatology 44:1218-1230.
23. Neumark YD, Friedlander Y, Durst R, Leitersdorf E, Jaffe D, Ramchandani VA, O'Connor S, Carr LG, Li TK (2004) Alcohol dehydrogenase polymorphisms influence alcohol-elimination rates in a male Jewish population. Alcohol Clin Exp Res 28:10-14.
24. Nishigaki R, Utsugi K, Maeda K, Inahara S, Enomoto K, Umemura K (1985) Effects of diethyldithiocarbamate, a metabolite of disulfiram, on the pharmacokinetics of alcohol and acetaldehyde in the rat. J Pharmacobiodyn 8:847-852.
25. Noll F (1984) Lactate, in Methods of Enzymatic Analysis (Bergmeyer HU ed) 3rd ed, pp. 582-587. Verlag Chemie, Basel.
26. Nuutinen HU, Salaspuro MP, Valle M, Lindros KO (1984) Blood acetaldehyde concentration gradient between hepatic and antecubital venous blood in ethanol-intoxicated alcoholics and controls. Eur J Clin Invest 14:306-311.
27. Rivera-Meza M, Quintanilla ME, Tampier L, Mura CV, Sapag A, Israel Y (2010) Mechanism of protection against alcoholism by an alcohol dehydrogenase polymorphism: development of an animal model. FASEB J 24:266-274.
28. Sachan DS, Cha YS (1994) Acetylcarnitine inhibits alcohol dehydrogenase. Biochem Biophys Res Commun 203:1496-1501.
29. Shimada J, Miyahara T, Otsubo S, Yoshimatsu N, Oguma T, Matsubara T (1987) Effects of alcohol-metabolizing enzyme inhibitors and beta-lactam antibiotics on ethanol elimination in rats. Jpn J Pharmacol 45:533-544.
30. Svensson S, Lundsjö A, Cronholm T, Höög JO (1996) Aldehyde dismutase activity of human liver alcohol dehydrogenase. FEBS Lett 394:217-220.
31. Tangerman A (1997) Highly sensitive gas chromatographic analysis of ethanol in whole blood, serum, urine, and fecal supernatants by the direct injection method. Clin Chem 43:1003-1009.
32. Thomasson HR, Beard JD, Li TK (1995) ADH2 gene polymorphisms are determinants of alcohol pharmacokinetics. Alcohol Clin Exp Res 19:1494-1499.
33. Tischler ME, Friedrichs D, Coll K, Williamson JR (1977a) Pyridine nucleotide distributions and enzyme mass action ratios in hepatocytes from fed and starved rats. Arch Biochem Biophys 184:222-236.
34. Tischler ME, Hecht P, Williamson JR (1977b) Determination of mitochondrial/cytosolic metabolite gradients in isolated rat liver cells by cell disruption. Arch Biochem Biophys 181:278-293.
35. Veech RL, Raijman L, Krebs HA (1970) Equilibrium relations between the cytoplasmic adenine nucleotide system and nicotinamide-adenine nucleotide system in rat liver. Biochem J 117:499-503.
36. Wall TL, Peterson CM, Peterson KP, Johnson ML, Thomasson HR, Cole M, Ehlers CL (1997) Alcohol metabolism in Asian-American men with genetic polymorphisms of aldehyde dehydrogenase. Ann Intern Med 127:376-379.
37. Waterlow JC, Garlick PJ, Millward DJ (1978) Protein Turnover in Mammalian Tissues and in the Whole Body. Elsevier/North-Holland, Amsterdam.
38. Williamson DH, Lund P, Krebs HA (1967) The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J 103:514-527.
39. Xiao Q, Weiner H, Crabb DW (1996) The mutation in the mitochondrial aldehyde dehydrogenase (ALDH2) gene responsible for alcohol-induced flushing increases turnover of the enzyme tetramers in a dominant fashion. J Clin Invest 98:2027-2032.
40. Xiao Q, Weiner H, Johnston T, Crabb DW (1995) The aldehyde dehydrogenase ALDH2*2 allele exhibits dominance over ALDH2*1 in transduced HeLa cells. J Clin Invest 96:2180-2186.
41. You M, Fischer M, Cho WK, Crabb D (2002) Transcriptional control of the human aldehyde dehydrogenase 2 promoter by hepatocyte nuclear factor 4: inhibition by cyclic AMP and COUP transcription factors. Arch Biochem Biophys 398:79-86.
42. Zorzano A, Ruiz del Arbol L, Herrera E (1989) Effect of liver disorders on ethanol elimination and alcohol and aldehyde dehydrogenase activities in liver and erythrocytes. Clin Sci (Lond) 76:51-57.
43. Zuurendonk PF, Tischler ME, Akerboom TP, Van Der Meer R, Williamson JR, Tager JM (1979) Rapid separation of particulate and soluble fractions from isolated cell preparations (digitonin and cell cavitation procedures). Methods Enzymol 56:207-223.