High-level expression of rat class I alcohol dehydrogenase is sufficient for ethanol-induced fat accumulation in transduced HeLa cells

Hepatology

Volumn 29, Issue 4, 1999, Pages 1164-1170

  Galli, Andrea ^a   Price, Donna ^b   Crabb, David ^b,c  

^a UNIVERSITY OF FLORENCE   (Italy)

^b INDIANA UNIVERSITY   (United States)

^c Emerson Hall 312 ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; ALPHA TOCOPHEROL; FATTY ACID; LACTIC ACID; LINOLEIC ACID; METHYLENE BLUE; PYRUVIC ACID; TRIACYLGLYCEROL;

ALCOHOL METABOLISM; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FATTY ACID OXIDATION; FATTY ACID TRANSPORT; FATTY LIVER; GENETIC TRANSDUCTION; HELA CELL; LIPID PEROXIDATION; LIPID STORAGE; LIPOGENESIS; LIVER METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

EID: 0032897276     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1002/hep.510290420     Document Type: Article

References (16)

1. Grunnet N, Kondrup J. The effect of ethanol on the beta oxidation of fatty acids. Alcohol Clin Exp Res 1986;10:64S-68S.
2. Lieber CS, Lefevere A, Spritz N, Feinman L, DeCarli LM. Difference in hepatic metabolism of long- and medium-chain fatty acids: the role of fatty acid chain length in the production of the alcoholic fatty liver. J Clin Invest 1967;46:1451-1460.
3. Grunnet N, Kondrup J, Dich J. Ethanol-induced accumulation of triacylglycerol in cultured hepatocytes: dependency on ethanol metabolism. Alcohol Alcohol 1987;Suppl 1:257-261.
4. DiLuzio NR. Prevention of the acute ethanol induced fatty liver by anti-oxidants. Physiologist 1963;6:169-180.
5. DiLuzio NR, Hartman AD. Role of lipid peroxidation in the pathogenesis of the ethanol induced fatty liver. Fed Proc 1969;26:1436-1442.
6. Morgan JC, DiLuzio NR. Inhibition of the acute ethanol induced fatty liver by pyrazole. Proc Soc Exp Biol Med 1970;134:462-466.
7. Muller A, Sies H. Role of alcohol dehydrogenase activity and the acetaldehyde in ethanol-induced ethane and pentane production by isolated perfused rat liver. Biochem J 1982;206:153-156.
8. Crabb DW, Bosron WF, Li T-K. Steady-state kinetic properties of purified rat liver alcohol dehydrogenase: application to predicting alcohol elimination rates in vivo. Arch Biochem Biophys 1983;224:299-309.
9. Kurose I, Higuchi H, Kato S, Miura S, Watanabe N, Kamegaya Y, Tomita K, et al. Oxidative stress on mitochondria and cell membrane of cultured hepatocytes and perfused liver exposed 10 ethanol. Gastroenterology 1997;112:1331-1343.
10. Bailey SM, Cunningham CC. Acute and chronic ethanol increases reactive oxygen species generation and decreases viability in fresh, isolated ral hepatocytes. HEPATOLOGY 1998;28:1318-1326.
11. Cederbaum AI. Microsomal generation of hydroxyl radicals: its role in microsomal ethanol oxidizing system (MEOS) activity and requirement for iron. Ann N Y Acad Sci 1987;492:35-49.
12. Wu DC, Cederbaum AI. Ethanol cytotoxicity to a transfected HepG2 cell line expressing human cytochrome P4502E1. J Biol Chem 1996;271:23914-23919.
13. Bradford BU, Forman DT, Thurman RG. 4-Methyl pyrazole inhibits fatty acyl coenzyme A synthetase and diminishes catalase-dependent alcohol metabolism: has the contribution of alcohol dehydrogenase to alcohol metabolism been previously overestimated? Mol Pharmacol 1993;43:115-119.
14. Dai Y, Cederbaum AI. Cytotoxicity of acetaminophen in human cytochrome P4502E1-transfected HepG2 cells. J Pharm Exp Ther 1995;273:1497-1505.
15. Clemens DL, Halgard CM, Miles RR, Sorrell MF, Tuma DJ. Establishment of a recombinant hepatic cell line stably expressing alcohol dehydrogenase. Arch Biochem Biophys 1995;321:311-318.
16. Mapoles JE, Iwahashi M, Lucas D, Zimmerman BT, Simon FR. Acetaldehyde exposure causes growth inhibition in a Chinese hamster ovary cell line that expresses alcohol dehydrogenase. Alcohol Clin Exp Res 1994;18:632-636.